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The effect of oral sodium taurocholate on endotoxemia and intestinal anastomotic wound healing in rats with obstructive jaundice

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Abstract

The effect of sodium taurocholate (ST) on endotoxemia and intestinal anastomotic wound healing in obstructive jaundice was evaluated in a rat model. A total of 108 Wistar rats were divided into three main groups. Thus, 36 animals were given ileal anastomosis (IA) alone (IA group), 36 were given IA with bile duct ligation (BDL) (IA+BDL group), and 36 were given IA with BDL and oral sodium taurocholate (ST) (IA+BDL+ST group). These three main groups were then divided into three equal subgroups, A, B, and C, which were killed on postoperative days (POD), 3, 5, and 9, respectively. In the IA+BDL+ST group, ST was administrated perioperatively and ceased from POD 5 onwards. The anastomotic hydroxyproline level and bursting pressure were significantly lower in the IA+BDL animals compared with the others on POD 3, 5, and 9 (P<0.008). Endotoxemia was prominent in the IA+BDL group from POD 3 (P=0.011). After ST was stopped, 42% of the AI+BDL+ST animals developed endotoxemia by POD 9 (P=0.008). Anastomotic wound healing was better in the IA+BDL+ST group (P<0.01). These findings suggest that endotoxemia and its adverse effects on wound healing in obstructive jaundice can be prevented by the oral administration of ST.

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References

  1. Wait RB, Kahng KU (1989) Renal failure complicating obstructive jaundice. Am J Surg 157:256–263

    Article  CAS  PubMed  Google Scholar 

  2. Armstrong CP, Dixon JM, Taylor TV, Dutes GG (1984) Surgical experience of deeply jaundiced patient with bile duct obstruction. Br J Surg 71:234–238

    CAS  PubMed  Google Scholar 

  3. Irwin TT, Vassilakis JS, Chattopadhyay DK, Greaney MG (1978) Abdominal wound healing in jaundiced patients. Br J Surg 65:521–522

    Google Scholar 

  4. Arroud JP, Humber W, Eloy MR, Adloft M (1981) Effect of jaundice on wound healing: An experimental study in rats. Am J Surg 141:593–596

    Google Scholar 

  5. Scott CCE, Grogar JB (1994) The pathophysiology of biliary obstruction and its effect on phagocytic and immune functions. J Surg Res 57:316–336

    Google Scholar 

  6. Greeve JW, Gouma BJ, Burman WA (1992) Complications in obstructive jaundice: Role of endotoxins. Scand J Gastroenterol 27, Suppl 194:8–12

    Google Scholar 

  7. Saitoh H, Hiraoko T, Uchino R, Miyauchi Y (1995) Endotoxemia and intestinal mucosal dysfunction after the relief of obstructive jaundice by internal and external drainage in rats. Eur J Surg 27:11–18

    CAS  Google Scholar 

  8. Bailey ME (1976) Endotoxins, bile acids and renal function in obstructive jaundice. Br J Surg 63:774–778

    CAS  PubMed  Google Scholar 

  9. Askew AR, Bates GJ, Balderson G (1984) Jaundice and effect of sodium taurocholate taken orally upon abdominal wound healing. Surg Gynecol Obstet 159:207–209

    CAS  PubMed  Google Scholar 

  10. Lewin J (1978) TheLimulus test: A status report. Prog Clin Diol Res 29:235–244

    Google Scholar 

  11. Jonsson K, Jiborn H, Zederfelat B (1983) Breaking strength of small intestine anastomoses. Am J Surg 145:800

    Article  CAS  PubMed  Google Scholar 

  12. Greenhalgh DG, Sprugel KH, Murray MJ, Ross R (1990) PDGF and FGF stimulate healing in the genetically diabetic mouse. Am J Surg 136:1235

    CAS  Google Scholar 

  13. Trapp RG (1990) Comparing three or more means. In: Trapp RG (ed) Basic and clinical biostatistics. Appleton & Lange, Norwalk, pp 124–141

    Google Scholar 

  14. Clement WD, Rowlands BJ (1993) Effects of extrahepatic obstructive jaundice on Kupffer cell clearance capacity. Arch Surg 128:200–205

    Google Scholar 

  15. Allison MEM, Prentice CRM, Kennedy AC (1979) Renal function and other factors in obstructive jaundice. Br J Surg 66:392–397

    CAS  PubMed  Google Scholar 

  16. Uchino R, Saito N, Hiraoka T, Tashiro S, Miyavaki Y (1991) Endotoxin-induced lung hemorrhages in obstructive jaundiced rats. Jpn J Surg 4:38–42

    Google Scholar 

  17. Greeve SW, Gouma BJ, Burman WA (1989) Bile acids inhibit endotoxin-induced release of tumor necrosis factor by monocytes: An in-vitro study. Hepatology 10:454–458

    Google Scholar 

  18. Regan MC, Kirk SJ, Hurson M (1991) Tumor necrosis factor-alfa inhibits in-vivo collagen synthesis. Surgery 113:173–177

    Google Scholar 

  19. Pain JA, Cahill CJ, Biley ME (1985) Perioperative complications in obstructive jaundice: Therapeutic considerations. Br J Surg 72:942–945

    CAS  PubMed  Google Scholar 

  20. Cahill CJ (1983) Prevention of postoperative renal failure in patients with obstructive jaundice: The role of bile salts. Br J Surg 70:590–595

    CAS  PubMed  Google Scholar 

  21. Pain JA, Cahill CJ, Johnson CD, Trapnell JE, Bailey ME (1991) Prevention of postoperative renal dysfunction in patients with obstructive jaundice: A multicentre study of bile salts and lactulose. Br J Surg 78:467–469

    CAS  PubMed  Google Scholar 

  22. Shands JW, Chun PW (1980) The dispersion of gram-negative lipopolysaccharide by deoxycholate. J Biol Chem 255:1221–1226

    CAS  PubMed  Google Scholar 

  23. Cahill CJ, Pain JA, Bailey ME (1987) Bile salts, endotoxin and renal function in obstructive jaundice. Surg Gynecol Obstet 165:519–522

    CAS  PubMed  Google Scholar 

  24. Gouma DJ, Coeldo JCU, Fisher JD, Sehlegel JF, Li YF, Moody FG (1986) Endotoxemia after relief of biliary obstruction by internal and external drainage in rats. Am J Surg 151:476–479

    Article  CAS  PubMed  Google Scholar 

  25. Grande L, Garcia JC, Valdecasas JC, Fuster J, Visa J, Pera C (1990) Obstructive jaundice and wound healing. Br J Surg 77:440–442

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Surgery, University of Istanbul, Cerrahpasa Medical School, Aksaray, Istanbul, Turkey

    Metin Sayan, Nihat Yavuz & Muzaffer Sariyar

  2. Department of Pathology, University of Istanbul, Cerrahpasa Medical School, Aksaray, Istanbul, Turkey

    Suha Göksel

  3. Department of Surgery, University of Kocaeli, Kocaeli Medical School, Sopaliçiftli≈gi, Kocaeli, Turkey

    Ahmet Alponat & Engin Altinkaya

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  1. Metin Sayan
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  2. Ahmet Alponat
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  4. Engin Altinkaya
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Sayan, M., Alponat, A., Yavuz, N. et al. The effect of oral sodium taurocholate on endotoxemia and intestinal anastomotic wound healing in rats with obstructive jaundice. Surg Today 27, 953–957 (1997). https://doi.org/10.1007/BF02388145

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  • DOI: https://doi.org/10.1007/BF02388145

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