Journal of tissue culture methods

, Volume 13, Issue 3, pp 179–184 | Cite as

Immunoselection and culture of cortical collecting duct cells

  • Anikó Náray-Fejes-Tóth
  • Géza Fejes-Tóth


A straightforward method is described for isolating and culturing cells from the renal cortical collecting duct. This method uses monoclonal antibodies and solid phase immunoadsorption to select for the targeted cell population. The large number of cells obtained with this method facilitates biochemical studies and initiation of cell cultures. Monolayers grown on permeable membranes can be used for studying mechanisms of ion transport and cell differentiation as well as their hormonal regulation.

Key words

immunoselection cell isolation monoclonal antibody cortical collecting duct primary cultures ion transport 


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V. References

  1. 1.
    Fejes-Tóth, G.; Náray-Fejes-Tóth, A. Differentiated transport functions in primary cultures of rabbit collecting ducts. Am. J. Physiol. 253:F1302-F1307; 1987.PubMedGoogle Scholar
  2. 2.
    Fejes-Tóth, G.; Náray-Fejes-Tóth, A. Isolated principal and intercalated cells: hormone responsiveness and Na/K-ATPase activity. Am. J. Physiol. 256:F742-F750; 1989.PubMedGoogle Scholar
  3. 3.
    Fejes-Tóth, G.; Náray-Fejes-Tóth, A. Fluorescence-activated cell sorting of collecting duct subtypes. J. Tissue Cult. Methods 13:173–178; 1991.Google Scholar
  4. 4.
    LeHir, M.; Kaissling, B.; Koeppen, B. M., et al. Binding of peanut lectin to specific epithelial cell types in kidney. Am. J. Physiol. 242:C117-C120; 1982.PubMedGoogle Scholar
  5. 5.
    Náray-Fejes-Tóth, A.; Carretero, O. A.; Fejes-Tóth, G. Effects of atrial natriuretic factor and vasopressin on cyclic nucleotides in cultured kidney cells. Hypertension 11:392–396; 1988.PubMedGoogle Scholar
  6. 6.
    Náray-Fejes-Tóth, A.; Fejes-Tóth, G. Glucocorticoid receptors mediate mineralocorticoid-like effects in cultured collecting duct cells. Am. J. Physiol. 259:F672-F678; 1990.PubMedGoogle Scholar
  7. 7.
    Schmiedlin-Ren, P.; Náray-Fejes-Tóth, A.; Fejes-Tóth, G. Regulation of K+ secretion in cultured collecting tubule cells: effects of AVP and luminal pH. Kidney Int. 33:422; 1988.Google Scholar
  8. 8.
    Smith, W. L.; Garcia-Perez, A. Immunodissection: use of monoclonal antibodies to isolate specific types of renal cells. Am. J. Physiol. 248:F1-F7; 1985.PubMedGoogle Scholar
  9. 9.
    Spielman, W. S.; Sonnenburg, W. K.; Allen, M. L., et al. Immunodissection and culture of rabbit cortical collecting tubule cells. Am. J. Physiol. 251:F348-F357; 1986.PubMedGoogle Scholar

Copyright information

© Tissue Culture Association 1991

Authors and Affiliations

  • Anikó Náray-Fejes-Tóth
    • 1
  • Géza Fejes-Tóth
    • 1
  1. 1.Department of PhysiologyDartmouth Medical SchoolHanover

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