Journal of tissue culture methods

, Volume 13, Issue 3, pp 179–184 | Cite as

Immunoselection and culture of cortical collecting duct cells

  • Anikó Náray-Fejes-Tóth
  • Géza Fejes-Tóth
Article

Summary

A straightforward method is described for isolating and culturing cells from the renal cortical collecting duct. This method uses monoclonal antibodies and solid phase immunoadsorption to select for the targeted cell population. The large number of cells obtained with this method facilitates biochemical studies and initiation of cell cultures. Monolayers grown on permeable membranes can be used for studying mechanisms of ion transport and cell differentiation as well as their hormonal regulation.

Key words

immunoselection cell isolation monoclonal antibody cortical collecting duct primary cultures ion transport 

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V. References

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Copyright information

© Tissue Culture Association 1991

Authors and Affiliations

  • Anikó Náray-Fejes-Tóth
    • 1
  • Géza Fejes-Tóth
    • 1
  1. 1.Department of PhysiologyDartmouth Medical SchoolHanover

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