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The Histochemical Journal

, Volume 25, Issue 11, pp 830–833 | Cite as

Immunofluorescent localization of platelet-activating factor (PAF) in the rat

  • Yutaka Hirashima
  • Ryoko Kato
  • Shunro Endo
  • Akira Takaku
  • Ken Karasawa
  • Shoshichi Nojima
Papers

Summary

An immunofluorescent staining method for detecting platelet-activating factor (PAF) is described. This method employs a polyclonal anti-PAF rabbit antibody. When rat brain, heart, lung, liver or kidney tissue was stained using this method, the heart, lung and kidney exhibited PAF-specific staining. Analysis of the amount of PAF in different organs, either by immunofluorescence or by bioassay, showed that kidney tissue contains the greatest amount of PAF.

Keywords

Immunofluorescent Staining Kidney Tissue Staining Method Rabbit Antibody Immunofluorescent Localization 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Chapman & Hall 1993

Authors and Affiliations

  • Yutaka Hirashima
    • 1
  • Ryoko Kato
    • 1
  • Shunro Endo
    • 1
  • Akira Takaku
    • 1
  • Ken Karasawa
    • 2
  • Shoshichi Nojima
    • 2
  1. 1.Department of NeurosurgeryToyama Medical and Pharmaceutical UniversityToyamaJapan
  2. 2.Faculty of Pharmaceutical SciencesTeikyo UniversityKanagawaJapan

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