Abstract
To demonstrate the cellular localization of prostaglandin E (PGE) in the normal and irritated rabbit iris, the indirect immunofluorescence method was employed. The amounts of PGE1 and PGF2α in the aqueous humor were determined with radioimmunoassay. Immunofluorescence of PGE was observed in the cytoplasm of mesenchymal cells of the iris. PGE-positive cells in the normal iris were scattered in the fibrous stroma, and the shape of these cells was oval to spindle-shaped. The numbers of these cells were remarkably increased in the iridectomized and inflamed iris. The amounts of PGE1 in the aqueous humor was 2.0±0.53 ng/ml in the normal eyes and 7.92±2.93 ng/ml in the irritated eyes. The mean level of PGF2α was 0.4±0.27 ng/ml in the normal eyes and 0.8±0.58 ng/ml in the irritated eyes.
Zusammenfassung
Um die celluläre Lokalisation von Prostaglandin E in normaler und entzündeter Iris darzustellen, wurde die Methode der indirekten Immunofluoreszenz angewandt. Außerdem stellte sich die Aufgabe, die Menge von PGE1 und PGF2α im Kammerwasser radioimmunologisch zu bestimmen. Die Immunofluoreszenz von PGE ließ sich im Cytoplasma der mesenchymalen Zellen der Iris beobachten. Die PGE-positiven Zellen lagen im Normalfall in die fibrillären Fasern des Sromas verstreut und zeigten eine ovale bis spindelartige Form. In der ausgeschnittenen und entzündeten Iris fand sich die Anzahl dieser Zellen merklich erhöht.-Die Menge der PGE1 im Kammerwasser betrug im normalen Auge 2,0±0,53 ng/ml und im gereizten Auge 7,92±2,93 ng/ml. Im normalen Auge lag der Durchschnittswert von PGF2α bei 0.4±0.27 ng/ml und im gereizten Auge bei 0.8±0.58 ng/ml.
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Sakata, H., Yoshida, H. Cellular localization of prostaglandin E in the rabbit iris by indirect immunofluorescence method. Albrecht v Graefes Arch. klin. exp. Ophthal. 211, 221–227 (1979). https://doi.org/10.1007/BF02387427
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DOI: https://doi.org/10.1007/BF02387427