Three-dimensional modeling of T-24 human bladder carcinoma cell line: A new simulated microgravity culture vessel
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We present a new device and method for culture of cell lines and primary tissues requiring high oxygen tensions. The High Aspect Rotating-Wall Vessel (HARV) described successfully propagated T-24, a human bladder transitional epithelial cell line, on Cytodex-3 microcarriers in three-dimensional cellular aggregates up to 0.5 cm in diameter. The HARV is a horizontally rotated tissue culture vessel with a large surface-area-to-volume ratio silicone membrane oxygenator. This design augments the principle of the rotating-wall vessel termed the Slow-Turning Lateral Vessel (STLV) by providing a low turbulence, low shear, cell growing environment with increased oxygen delivery capability. Comparisons of glucose metabolism, oxygen consumption, and morphology as a function of cell growth for a T-24 bladder carcinoma were performed in the HARV vs. the STLV. The HARV was superior in the culture of a variety of cell types including normal and neoplastic, anchorage-dependent and suspension cells.
Key wordsthree-dimensional cell culture microcarrier cell culture T-24 cells rotating-wall vessel oxygen delivery
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- 1.Dedolph, R. R.; Dipert, M. H. The physical basis of gravity nullification by clinostatic rotation. Plant Physiol. 47(6):756–764; 1971.Google Scholar
- 3.Ganong, W. F. Review of medical physiology. East Norwalk, CT: Appleton & Lange; 1989.Google Scholar
- 4.Goodwin, T. J.; Schroeder, W. F.; Wolf, D. A., et al. Rotating-wall vessel coculture of small intestine as a prelude to tissue modeling: Aspects of simulated microgravity. In press; 1993.Google Scholar
- 6.Goodwin, T. J.; Jessup, J. M.; Sams, C., et al. In vitro three-dimensional tissue modeling. JSC Annual Report. NASA Technical Memorandum 100473; 1988.Google Scholar
- 8.Pugh, G. G. The role of oxygen consumption rate in bioreactor process control. Biotechnology 6:524–526; 1988.Google Scholar
- 9.Shibayama, D.; Mashimo, T. U.S. Patent No. 3,676,074; 1972.Google Scholar
- 11.Schwarz, R. P.; Wolf, D. A.; Trinh, T. T. U.S. Patent No. 5,026,650; 1991.Google Scholar
- 12.Tsao, Y. D.; Goodwin, T. J.; Wolf, D. A., et al. Responses of gravity level variations on the NASA/JSC bioreactor system. Physiologist 35(1):49–50; 1992.Google Scholar
- 13.Wolf, D. A.; Schwarz, R. P.; Trinh, T. Controlled turbulence bioreactors. NASA Tech Briefs; October 1989:74.Google Scholar