Pediatric Radiology

, Volume 16, Issue 2, pp 144–149 | Cite as

Comparison of in vivo31P-MR spectra of the brain, liver, and kidney of adult and infant animals

  • H. C. Schmidt
  • C. A. Gooding
  • T. L. James
  • R. Gonzalez-Mendez
  • J. L. James


In vivo31P-magnetic resonance spectroscopy (MRS) was used to determine the phosphorus metabolite levels in the brain and kidney of infant rabbits and adult rats and in the liver of infant rabbits and adult and infant rats. For31P-MRS of the brain, a surface, radiofrequency coil was placed on the anterosuperior region of the head; for31P-MRS of the liver and kidney, a radiofrequency coil was chronically implanted either between the hepatic lobes or around the kidney.31P-MR spectra were found to show large variations in the levels of the phosphorus metabolites depending on the species, the organ, and the age of the animal. The phosphate monoester (MP)/adenosine triphosphate (ATP) ratio was significantly higher and the phosphocreatine (PCr)/ATP ratio was significantly lower in the brains of infant rabbits than in the brains of adult rats. Comparison of these data with data reported for humans and other animals suggests that these differences are due mainly to differences in age and not to differences among species. The phosphodiester (PD)/ATP ratio was found to be significantly higher in the livers of infant rabbits than in the livers of adult and infant rats — a difference more likely related to the species than to age. The kidneys of the infant rabbits showed a higher PCr/ATP radio than the kidneys of the adult rats, but this difference might be due to the influence of PCr in the surrounding muscle.


Phosphate Phosphorus Adenosine Triphosphate Resonance Spectroscopy 
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  1. 1.
    James TL, Gonzalez-Mendez R, Litt L (1984) Magnetic resonance spectroscopy: Principles and potential applications in medicine. In: Goldberg HI, Higgins CB, Ring EJ, (eds) Contemporary imaging. Radiology Research and Education Foundation, San Francisco, p 141Google Scholar
  2. 2.
    Seo Y, Murakami M, Watari H, Imai Y, Yoshizaki K, Nishikawa H, Morimoto T (1983) Intracellular pH determination by a31P-NMR technique. The second dissociation constant of phosphoric acid in a biological system. J Biochem 94: 729PubMedGoogle Scholar
  3. 3.
    Hope P, Reynolds E (1985) Investigation of cerebral energy metabolism in newborn infants by phosphorus nuclear magnetic resonance spectroscopy. Clin Perinatol 12: 261PubMedGoogle Scholar
  4. 4.
    Ackerman J, Grove T, Wong G, Gadian D, Radda G (1980) Mapping of metabolites in whole animals by31P NMR using surface coils. Nature 283: 167CrossRefPubMedGoogle Scholar
  5. 5.
    Younkin D, Delivoria-Papadopoulos M, Leonard J, Subramanian V, Eleff S, Leigh J, Chance B (1984) Unique aspects of human newborn cerebral metabolism evaluated with phosphorus nuclear magnetic resonance spectroscopy. Ann Neurol 16: 581CrossRefPubMedGoogle Scholar
  6. 6.
    Ross B, Radda G, Gadian D, Rocker G, Esiri M, Falconer-Smith J (1981) Examination of a case of suspected McArdle's syndrome by31P nuclear magnetic resonance. N Engl J Med 304: 1338PubMedGoogle Scholar
  7. 7.
    Chance B, Eleff S, Bank W, Leigh J, Warnell R (1982)31P NMR studies of control of mitochondrial function in phosphofructokinase-deficient human skeletal muscle. Proc Natl Acad Sci USA 79: 7714PubMedGoogle Scholar
  8. 8.
    Edwards R, Wilkie D, Dawson M, Gordon R (1982) Clinical use of nuclear magnetic resonance in the investigation of myopathy. Lancet 1: 725PubMedGoogle Scholar
  9. 9.
    Reynolds E, Hope P, de L Costello A, Cady E, Delpy D, Tofts P, Hamilton P, Chu A, Wilkie D (1984)31P NMR spectroscopy of the brain in newborn infants. Proceedings of the Third Annual Meeting of the Society of Magnetic Resonance in Medicine, New York, N.Y., Aug. 13–17. Research Medicine Group, Donnor Laboratory, Lawrence Berkeley Laboratory, University of California, Berkeley, p 617Google Scholar
  10. 10.
    Gonzalez-Mendez R, McNeill A, Gregory G, Wall S, Gooding C, Litt L, James T (1985) The effects of hypoxia on cerebral phosphate metabolites and pH in the anesthetized infant rabbit. J Cerebral Blood Flow Metab (in press)Google Scholar
  11. 11.
    Bottomly P, Hart H, Edelstein W, Schenck J, Smith L, Leue W, Mueller O, Redington R (1983) NMR imaging spectroscopy system to study both anatomy and metabolism. (Letter to the editor) Lancet II: 273Google Scholar
  12. 12.
    Bottomly P, Hart H, Edelstein W, Schenck J, Smith L, Leue W, Mueller O, Redington R (1984) Anatomy and metabolism of the normal human brain studied by magnetic resonance at 1.5 tesla. Radiology 150: 441Google Scholar
  13. 13.
    Radda G, Shulman R (1984) Nuclear magnetic resonance of in vivo metabolism: From normal to pathophysiology. In: James T, Margulis A (eds) Biomedical magnetic resonance. Radiology Research and Education Foundation, San Francisco, p 201Google Scholar
  14. 14.
    Koretsky A, Wang S, Murphy-Boesch J, Klein M, James T, Weiner M (1983)31P NMR spectroscopy of rat organs, in situ, using chronically implanted radiofrequency coils. Proc Natl Acad Sci USA 80: 7491PubMedGoogle Scholar
  15. 15.
    Schmidt H, Gooding C, James T (1985) In vivo31P-MR spectroscopy of the liver in the infant rabbit to study the effect of hypoxia on the phosphorus metabolites and intracellular pH. Invest Radiol (in press)Google Scholar
  16. 16.
    Hochachka PW, Somero GN (1984) Biochemical adaptation. Princeton University Press, Princeton, p 250Google Scholar
  17. 17.
    Murphy-Boesch J, Koretsky A (1983) An in vivo NMR probe circuit for improved sensitivity. J Magn Reson 54: 526Google Scholar
  18. 18.
    Gonzalez-Mendez R, Litt L, Koretsky A, von Colditz J, Weiner M, James T (1984) Comparison of31P NMR spectra of in vivo rat brain using convolution difference and saturation with a surface coil. Source of the broad component in the brain spectrum. J Magn Reson 57: 526Google Scholar
  19. 19.
    Ackerman J, Evelhoch J, Berkowitz B, Kichura G (1984) Selective suppression of the cranial bone resonance from31P NMR experiments with rat brain in vivo. J Magn Reson 56: 318Google Scholar
  20. 20.
    Hoult D, Busby S, Gadian D, Radda G, Richards R, Seeley P (1974) Observation of tissue metabolites using31P nuclear magnetic resonance. Nature 252: 285CrossRefPubMedGoogle Scholar
  21. 21.
    Quistorff B, Engkagul A, Chance B (1983)31P-NMR in the study of liver metabolism in vivo. Pharmacol Biochem Behav 18 (Suppl 1): 241PubMedGoogle Scholar
  22. 22.
    Cohen S (1983) Simultaneous13C and31P NMR studies of perfused rat liver. J Biol Chem 258: 14294PubMedGoogle Scholar
  23. 23.
    Cohen S (1983) Application of nuclear magnetic resonance to the study of liver physiology and disease. Hepatology 3: 738PubMedGoogle Scholar
  24. 24.
    Norwood W, Ingwall J, Norwood C, Fossel E (1983) Developmental changes of creatine kinase metabolism in rat brain. Am J Physiol 244: C205Google Scholar
  25. 25.
    Siesjo B (1978) Brain energy metabolism. John Wiley and Sons, New York, p 398Google Scholar
  26. 26.
    Litt L, Gonzalez-Mendez R, Weinstein PR, Hashimoto T, Severinghaus JW, Murphy-Boesch J, James TL (1984) Hypoxic hypoxia and global ischemia in the brain: Correlation of physiological parameters with in vivo31P NMR spectral changes. Anesthesiol 61: A318Google Scholar
  27. 27.
    Tofts P, Wray S (1984) The time course of phosphorus-metabolite changes in developing rat brain estimated by31P nuclear magnetic resonance spectroscopy (NMRS). (Proceedings of the Physiological Society) J Physiol 353: 123 PGoogle Scholar
  28. 28.
    Cady E, Dawson M, Hope P, Tofts P, de L Costello A, Delpy D, Reynolds E, Wilkie D (1983) Non-invasive investigation of cerebral metabolism in newborn infants by phosphorus nuclear magnetic resonance spectroscopy. Lancet I: 1059Google Scholar
  29. 29.
    Bolinger L, Gyulai L, Chance B, Leigh J (1984) Identification of a major component of the monoester region of the phosphorus NMR spectra of neonate and puppy brain. Proceedings of the Third Annual Meeting of the Society of Magnetic Resonance in Medicine, New York, N.Y., Aug. 13–17. Research Medicine Group, Donnor Laboratory, Lawrence Berkeley Laboratory, University of California, Berkeley, p 59Google Scholar
  30. 30.
    Chaikoff IL (1942) The application of labelling agent to the study of phospholipid metabolism. Physiol Rev 22: 291Google Scholar
  31. 31.
    Glonek T, Kopp S, Kot E, Pettegrew J, Harrison W, Cohen M (1982) P-31 nuclear magnetic resonance analysis of brain: The perchloric acid extract spectrum. J Neurochem 39: 1210PubMedGoogle Scholar
  32. 32.
    McLaughlin A, Takeda H, Chance B (1979) Rapid ATP assays in perfused mouse liver by31P NMR. Proc Natl Acad Sci USA 76: 5445PubMedGoogle Scholar
  33. 33.
    Hems D, Brosnan J (1970) Effects of ischaemia on content of metabolites in rat liver and kidney in vivo. Biochemistry 120: 105Google Scholar
  34. 34.
    Miller A, Shamban A (1977) A comparison of methods for stopping intermediary metabolism of developing rat brain. J Neurochem 28: 1327PubMedGoogle Scholar
  35. 35.
    Radda G, Ackerman J, Bore P, Sehr P, Wong G, Ross B, Green Y, Bartlett S, Lowry M (1980)31P NMR studies on kidney intracellular pH in acute renal acidosis. Int J Biochem 12: 277CrossRefPubMedGoogle Scholar
  36. 36.
    Ackerman J, Lowry M, Radda G, Ross B, Wong G (1981) The role of intrarenal pH in regulation of ammoniagenesis: [31P] NMR studies of the isolated perfused rat kidney. J Physiol 319: 65PubMedGoogle Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • H. C. Schmidt
    • 1
    • 2
  • C. A. Gooding
    • 1
  • T. L. James
    • 1
    • 3
  • R. Gonzalez-Mendez
    • 4
  • J. L. James
    • 5
  1. 1.Department of RadiologyUniversity of California School of MedicineSan FranciscoUSA
  2. 2.Klinikum GroßhadernMünchenFRG
  3. 3.Department of Pharmaceutical ChemistryUCSFUSA
  4. 4.Department of AnesthesiologyUCSFUSA
  5. 5.Department of PathologyUCSFUSA

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