Skip to main content
SpringerLink
Log in

Variability in the distribution of selenium in healthy heart tissue measured by neutron activation analysis

  • Applications of Radioanalytical Methods to Biological and Clinical Systems
  • Published:
Journal of Radioanalytical and Nuclear Chemistry Aims and scope Submit manuscript

Abstract

Chronic dietary deficiency of selenium has been shown to be associated with degenerative heart disease in production animals in the U.S. and in the human in parts of China. In the latter, subjects in the endemic areas suffer high rates of a cardiomyopathy known as Keshan's Disease which is normally fatal in early adulthood and can be prevented, or reversed in its early stages, via selenium supplementation. Selenium, as the active moiety in the enzyme glutathione peroxidase, protects against oxidative attack of cell membranes by peroxides formed during normal metabolism. In this study, we investigated the distribution of selenium in healthy porcine and bovine heart tissue freshly collected at slaughter. The whole heart was perfused with DI water and carefully de-fatted. Representative samples of left and right atria and ventricles and the interventricular septum were collected, lyophilized and homogenized prior to preparing replicate samples for analysis. Replicates were analyzed for selenium via an INAA scheme employing a 5, 15 and 25 second irradiation (φth = 8·1013 n·cm−2·s−1), decay and real-time count (77mSe,T 1/2=17.4 s), respectively, using high-resolution gamma-ray spectroscopy with Westphal pulse pile-up correction. Selenium distribution will be discussed relative to differentiated function and oxygenation of the specific tissues.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. K. Schwartz, C. M. Foltz, J. Amer. Chem. Soc., 79 (1957) 3292.

    Google Scholar 

  2. K. Schwartz, Proc. Soc. Exp. Biol. Med., 78 (1951) 852.

    Google Scholar 

  3. H. E. Ganther, D. G. Hafeman, R. A. Lawrence, R. E. Serfass, W. G. Hoekstra, in: Trace Elements in Human Health and Disease, a Review,A. S. Prasad, D. Oberleas (Eds), Academic Press, New York, 1976.

    Google Scholar 

  4. J. Spallholz, J. Martin, H. Ganther (Eds), Proc. 2nd Intern. Symp. on Selenium in Biology and Medicine, May, 1980, AVI Publishing Company, 1981.

  5. G. Combs Jr., O. Levander, J. Spallholz, J. Oldfield (Eds), Proc. 3nd Intern. Symp. on Selenium in Biology and Medicine, May, 1984, AVI Publishing Company, 1987.

  6. A. Wendel (Ed.), Proc. 4th Intern. Symp. on Selenium in Biology and Medicine, July, 1980, Springer-Verlag, 1989.

  7. G. C. Mills, J. Biol. Chem., 229 (1957) 189.

    CAS  Google Scholar 

  8. J. T. Rotruck, A. L. Pope, H. E. Ganther, A. B. Swanson, D. G. Hafeman, W. G. Hoekstra, Science, 179 (1973) 588.

    CAS  Google Scholar 

  9. S. H. Oh, H. E. Ganther, W. G. Hoekstra, Biochem., 13 (1974) 1825.

    CAS  Google Scholar 

  10. J. W. Forstrom, J. J. Zakowski, A. L. Tappel, Biochem., 17 (1978) 2639.

    CAS  Google Scholar 

  11. M. J. Berry, L. Banu, P. R. Larsen, Nature, 349 (1991) 438.

    Article  CAS  Google Scholar 

  12. M. A. Motsenbocker, A. L. Tappel, Biochim. Biophys. Acta, 719 (1982) 147.

    CAS  Google Scholar 

  13. J. G. Yang, J. Morrison-Plummer, R. F. Burk, J. Biol. Chem., 262 (1987) 13372.

    CAS  Google Scholar 

  14. J. T. Deagen, M. A. Beilstein, P. D. Whanger, J. Inorg. Biochem., 41 (1991) 261.

    Article  CAS  Google Scholar 

  15. I. Harrison, D. Littlejohn, G. S. Fell, Analyst, 121 (1996) 189.

    CAS  Google Scholar 

  16. G. A. Colditz, JAMA, 276 (1996) 1984.

    Article  CAS  Google Scholar 

  17. L. C. Clark, G. F. Combs, B. W. Turnbull, E. H. Slate, D. K. Chalker, J. Chow, L. S. Davis, R. A. Glover, G. F. Graham, E. G. Gross, A. Krongrad, J. L. Lesher Jr., H. K. Park, B. B. Sander Jr, C. L. Smith, J. R. Taylor, JAMA, 276 (1996) 1957.

    Article  CAS  Google Scholar 

  18. G. Yang, K. Ge, J. Chen, X. Chen, World Rev. Nutr. Diet., 55 (1988) 98.

    CAS  Google Scholar 

  19. J. T. Salonen, G. Alfthan, J. K. Huttunen, J. Pikkarainen, P. P. Puska, Lancet, 2 (1982) 175.

    CAS  Google Scholar 

  20. P. V. Luoma, E. A. Sotaniemi, H. Korpela, J. Kumpulainen, 46 (1984) 469.

    CAS  Google Scholar 

  21. G. Guidi, R. Schiavon, I. Sheiban, G. Perona, Scandinavian J. Clin. Lab. Invest., 46 (1986) 549.

    CAS  Google Scholar 

  22. O. Oster, M. Drexler, J. Schenk, T. Meinertz, W. Kasper, C. J. Schuster, W. Prellwitz, Ann. Clin. Res., 18 (1986) 36.

    CAS  Google Scholar 

  23. J. T. Salonen, J. K. Huttunen, Ann. Clin. Res., 18 (1986) 30.

    CAS  Google Scholar 

  24. J. T. Salonen, R. Salonen, M. Ihanainen, M. Parviainen, R. Seppanen, M. Kantola, K. Seppanen, R. Rauramaa, Amer. J. Clin. Nutr., 48 (1988): 1226.

    CAS  Google Scholar 

  25. H. Koehler, H. J. Peters, H. Pankau, H. J. Duck, Biol. Trace Elem. Res., 15 (1988) 157.

    CAS  Google Scholar 

  26. F. J. Kok, A. Hofman, J. C. M. Witteman, A. M. Debruijn, D. H. Kruyssen, JAMA, 262 (1989) 1161.

    Google Scholar 

  27. O. A. Levander, Ann. Rev. Nutr., 7 (1987) 226.

    Google Scholar 

  28. J. T. Salonen, Intern. J. Epidemiol., 16 (1987) 323.

    CAS  Google Scholar 

  29. J. Ringstad, B. K. Jacobsen, Y. Thomassen, J. Trace Elem. Electrolytes Health Disease, 1 (1989) 27.

    Google Scholar 

  30. B. Akesson, B. Steen, Comprehensive Gerontology. Sect. A, Clin. Lab. Sci., 1 (1987) 61.

    CAS  Google Scholar 

  31. P. Auzepy, M. Blondeau, C. Richard, D. Pradeau, P. Therond, T. Thuone, Acta Cardiol., 42 (1987) 161.

    CAS  Google Scholar 

  32. M. L. Jackson, Biol. Trace Elem. Res., 15 (1988) 13.

    CAS  Google Scholar 

  33. W. C. Reeves, S. P. Marcuard, S. E. Willis, A. Movahed, J. Par. Ent. Nutr., 13 (1989) 663.

    CAS  Google Scholar 

  34. R. Beaglehole, R. Jackson, J. Watkinson, R. Scragg, R. L. Yee, Int. J. Epidemiol., 19 (1990) 918.

    CAS  Google Scholar 

  35. S. G. F. Bukkens, N. De Vos, F. J. Kok, E. G. Schouten, A. M. De Brujin, A. Hofman, 9 (1990) 128.

    CAS  Google Scholar 

  36. O. Oster, W. Prellwitz, Biol. Trace Elem. Res., 24 (1990) 91.

    CAS  Google Scholar 

  37. P. Suadicani, H. O. Hein, F. Gyntelberg, Atherosclerosis, 96 (1992) 33.

    Article  CAS  Google Scholar 

  38. J. P. Thomas, P. C. Geiger, A. W. Girotti, J. Lipid Res., 34 (1993) 479.

    CAS  Google Scholar 

  39. S. Pucheu, C. Coudray, G. Vanzetto, A. Favier, J. Machecourt, J. Deleiris, Biol. Trace Elem. Res., 47 (1995) 171.

    CAS  Google Scholar 

  40. R. Thiele, M. Schuffenhauer, K. Winnefeld, H. Dawcynski, J. Pleissner, R. Pfeifer, Medizinische Klinik, 90 (Suppl. 1) (1995) 45.

    Google Scholar 

  41. J. F. Sullivan, A. J. Blotcky, M. M. Jetton, H. K. Hahn, R. E. Burch, J. Nutr., 109 (1997) 1432.

    Google Scholar 

  42. C. D. Thomson, M. F. Robinson, Am. J. Clin. Nutr., 33 (1980) 303.

    CAS  Google Scholar 

  43. T. A. Miettinen, G. Alfthan, J. K. Huttunen, J. Pikkarainen, V. Naukkarinen, S. Mattila T. Kumlin, 287 (6391) (1983) 517.

  44. B. Lloyd, R. S. Lloyd, B. E. Clayton, J. Clin. Path., 37 (2) (1984) 200.

    Google Scholar 

  45. J. Virtamo, E. Valkeila, G. Affthan, S. Punsar, J. K. Huttunen, M. J. Karvonen, Amer. J. Epidemiol., 122 (2) (1985) 276.

    CAS  Google Scholar 

  46. J. T. Salonen, Int. J. Epidemiol., 16(2) (1987) 323.

    CAS  Google Scholar 

  47. J. T. Salonen, R. Salonen, K. Seppanen, M. Kantola, M. Parviainen, G. Alfthan, P. H. Maenpaa, E. Taskinen, R. Rauramaa, Atherosclerosis, 70 (1–2) (1988) 155.

    CAS  Google Scholar 

  48. M. F. Robinson, Nutr. Rev., 47 (4) (1989) 99.

    CAS  Google Scholar 

  49. H. Ikram, I. G. Crozier, M. Webster, C. J. Low, New Zealand Med. J., 102(863) (1989) 100.

    CAS  Google Scholar 

  50. R. A. Riemersma, M. Oliver, R. A. Elton, G. Alfthan, E. Vartiainen, M. Salo, P. Rubba, M. Mancini, H. Georgi, J. P. Vuilleumier, European J. Clin. Nutr., 44 (1990) 143.

    CAS  Google Scholar 

  51. S. G. Bukkens, N. De Vos, F. J. Kok, E. G. Schouten, A. M. De Bruijn, A. Hofman, J. Amer. College of Nutr., 9 (1990) 128.

    CAS  Google Scholar 

  52. F. Jossa, M. Trevisan, V. Krogh, E. Farinaro, D. Giumetti, G. Fusco, R. Galasso, S. Panico, S. Frascatore, C. Mellone, Atherosclerosis, 87(2–3) (1991) 129.

    CAS  Google Scholar 

  53. W. J. Blot, R. Wu, U. De Faire, A. R. Lefvert, J. Swedenborg, Arterioscler. Thromb. Vasc. Biol., 15 (1995) 441.

    Google Scholar 

  54. S. Salvini, C. H. Hennekens, J. S. Morris, W. C. Willett, M. J. Stampfer, Amer. J. Card., 76 (1995) 1218.

    CAS  Google Scholar 

  55. J. Neve, J. Cardiovascular Risk, 3 (1996) 42.

    CAS  Google Scholar 

  56. National Research Council, 1980, Recommended Dietary Allowances, 9th ed., National Academy Press, Washington, D.C.

    Google Scholar 

  57. O. A. Levander, P. D. Wanger, J. Nutr., 126 (1996) 2427S.

    Google Scholar 

  58. Standing Nordic Committee on Food, 1989, Nordic Nutrition Recommendations, Nordic Council of Ministers, Copenhagen, Denmark.

    Google Scholar 

  59. Scientific Committee for Food, 1993, Nutrient and Energy Intakes for the European Community, Commission of the European Communities, Brussels, Belgium.

    Google Scholar 

  60. Department of Health, 1991, Dietary Reference Values for Food Energy and Nutrients for the United Kingdom (Report on Health and Social Subjects, No. 41), HMSO, London, UK.

    Google Scholar 

  61. Committee on Nutritional Requirements, 1991, Recommendations on Nutrient Intake, 5th rev. ed., German Society of Nutrition, Frankfurt, Germany.

    Google Scholar 

  62. National Research Council, 1989, Recommended Dietary Allowances, 10th ed., National Academy Press, Washington, D.C.

    Google Scholar 

  63. FDA, 1995, Food Labeling: Reference Daily Intakes. Fed. Reg. 60:67164.

    Google Scholar 

  64. G. Yang, R. Zhou, S. Yin, L. Gu, B. Yan, Y. Liu, X. Li, J. Trace Elem. Electrolytes Health Dis., 3 (1989) 7787.

    Google Scholar 

  65. G. Yang, R. Zhou, S. Yin, L. Gu, B. Yan, Y. Liu, X. Li, J. Trace Elem. Electrolytes Health Dis., 3 (1989) 123.

    CAS  Google Scholar 

  66. M. P. Longnecker, P. R. Taylor, O. A. Levander, S. M. Howe, C. Veillon, P. A. Mcadam, K. Y. Patterson, J. M. Holden, M. J. Stampfer, J. S. Morris, W. C. Willett, Am. T. Clin. Nutr., 53 (1991) 1288.

    CAS  Google Scholar 

  67. K. A. Poirier, in: Risk Assessment of Essential Elements,W. Mertz, andC. O. Abernathy (Eds), ILSI Press, Washington, D.C., 1994, p. 157.

    Google Scholar 

  68. G. F. Combs, Jr., in: Risk Assessment of Essential Elements,W. Mertz, andC. O. Abernathy (Eds), ILSI Press, Washington, D.C., 1994, p. 167.

    Google Scholar 

  69. T. W. Graham, Vet. Clin. of North Amer. — Food Animal Pract., 7 (1991) 153.

    CAS  Google Scholar 

  70. G. D. Cawley, R. Bradley, Vet. Rec., 103 (1978) 239.

    CAS  Google Scholar 

  71. S. Kennedy, D. A. Rice, W. B. Davidson, Res. in Vet. Sci., 43 (1987) 384.

    CAS  Google Scholar 

  72. J. F. Van Vleet, Proc. 3nd Intern. Symp.on Selenium in Biology and Medicine, May, 1984, pp. 715–732,G. Combs Jr., O. Levander, J. Spallholz, J. Oldfield (Eds) AVI Publishing Company, 1987.

  73. K. B. Pedersen, M. G. Simesen, Nordisk Veterinaermedicin, 29 (1977) 161.

    CAS  Google Scholar 

  74. P. T. Jensen, Nordisk Veterinaermedicin, 29 (1977) 166.

    CAS  Google Scholar 

  75. D. C. Moir, H. G. Masters, Australian Veterinary J., 55 (1979) 360.

    CAS  Google Scholar 

  76. D. A. Rice, S. Kennedy, Amer. J. of Vet. Res., 50 (1989) 2101.

    CAS  Google Scholar 

  77. T. K. Nielsen, C. Wolstrup, A. L. Schirmer, P. Y. Jensen, Vet. Rec., 124 (1989) 535.

    CAS  Google Scholar 

  78. H. Korpela, Intern. J. Vit. Nutr. Res., 60 (1990) 156.

    CAS  Google Scholar 

  79. L. G. Young, J. H. Lunsden, A. Lun, J. Claxton, D. E. Edmeades, Canadian J. Comp. Med., 40 (1976) 92.

    CAS  Google Scholar 

  80. T. B. Goehring, I. S. Palmer, O. E. Olson, G. W. Libal, J. Anim. Sci., 59 (1984) 725.

    CAS  Google Scholar 

  81. O. Oster, M. Dahm, H. Oelert, W. Prellwitx, Clin. Chem., 35 (1989) 851.

    CAS  Google Scholar 

  82. O. Oster, M. Dahm, H. Oelert, European Heart J., 14 (1993) 770.

    CAS  Google Scholar 

  83. L. D. Koller, J. H. Exon, Can. J. Vet. Res., 50 (1986) 297.

    CAS  Google Scholar 

  84. J. S. Morris, M. F. Smith, R. E. Morrow, E. D. Heimann, J. C. Hancock, T. Gall, J. Radioanal. Chem., 69 (1982) 473.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. University of Missouri Research Reactor Center, University of Missouri-Columbia, 65211, Columbia, MO, USA

    C. J. Tharp, J. K. Kelly, J. S. Morris, C. K. Baskett, V. L. Spate, M. M. Mason, T. P. Cheng & T. A. Nichols

Authors
  1. C. J. Tharp
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. K. Kelly
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. S. Morris
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C. K. Baskett
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. V. L. Spate
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. M. Mason
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. T. P. Cheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. T. A. Nichols
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tharp, C.J., Kelly, J.K., Morris, J.S. et al. Variability in the distribution of selenium in healthy heart tissue measured by neutron activation analysis. J Radioanal Nucl Chem 236, 57b–63 (1998). https://doi.org/10.1007/BF02386318

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02386318

Keywords

Search

Navigation