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Effects of granulocyte colony-stimulating factor on bacterial translocation due to burn wound sepsis

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Abstract

The presence of certain defects in both cellular and humoral immunity after thermal injury has been established. Likewise, the translocation of enteric bacteria to the mesenteric lymph nodes and to distant organs has also been observed following serious thermal injury. The effects of granulocyte colony-stimulating factor (G-CSF) on bacterial translocation, the small bowel mucosa, and cecal bacterial content were investigated in a rat model of burn wound sepsis in which albino Wistar rats were scalded over 30% of their bodies, after which the lesions were infected by 1×108 colony-forming units (cfu)Pseudomonas aeruginosa. The control group was treated with 5% dextrose solution subcutaneously starting 2 days preburn, while the treatment group received 100μg/kg human G-CSF subcutaneously. On the 4th day post burn all animals were killed to examine the bowel and culture of the mesenteric lymph nodes (MLN), livers, and spleens. No significant differences were observed between the groups regarding the cecal bacterial content and small bowel; however, a difference was seen in the ratio of translocation in the MLN liver and spleen and quantitative MLN cultures. Based on these findings, G-CSF was thus found to be significantly effective in reducing bacterial translocation due to burn wound sepsis.

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Authors and Affiliations

  1. Department of Surgery, Istanbul Taksim Hospital, Straseluilere cab., 80060, Beyoǵh Istanbul, Turkey

    Orhan Yalçin, Gürsel Soybir, Ferda Köksoy & Hakki Köse

  2. Department of Plastic and Reconstructive Surgery, Istanbul Taksim Hospital, Straseluilere cad., 80060, Beyoǵh Istanbul, Turkey

    Baki Çokne§eli

  3. Department of Microbiology, Cerrahpasa Medical School, Istanbul University, Kocamustafa pa§a, Istanbul, Turkey

    Recep Öztürk

Authors
  1. Orhan Yalçin
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  2. Gürsel Soybir
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  3. Ferda Köksoy
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  4. Hakki Köse
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  5. Recep Öztürk
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  6. Baki Çokne§eli
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Yalçin, O., Soybir, G., Köksoy, F. et al. Effects of granulocyte colony-stimulating factor on bacterial translocation due to burn wound sepsis. Surg Today 27, 154–158 (1997). https://doi.org/10.1007/BF02385906

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  • DOI: https://doi.org/10.1007/BF02385906

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