Abstract
This paper describes our method for producing a rat model with an isolated bladder in situ in which the bladder makes no contact with urine. First, the right kidney was removed, then an external catheter was placed in the right ureter for bladder infusions, and next the left ureter was anatomosed to the proximal part of the descending colon. The animals were treated with antibiotics, and saline was infused daily into the bladder in order to prevent atrophy. This in situ model is considered to be useful in studies investigating the influence of specific compounds, such as carcinogens, on the bladder and its urothelium.
This is a preview of subscription content, log in via an institution to check access.
References
Cohen SM, Greenfield RE, Ellwein LB (1983) Multistage carcinogenesis in the urinary bladder. Environm Health Perspect 49:209–215
Airoldi L, Bonfati M, Magagnotti C, Fanelli R (1987) Development of an experimental model for studying bladder carcinogen metabolism using the isolated urinary bladder. Cancer Res 47:3697–3700
Messing EM, Hansson P, Ulrich P, Erturk E (1987) Epidermal growth factor-interactions with normal and malignant urothelium: in vivo and in situ studies. J Urol 138:1329–1335
Ravery V, Jouanneau J, Gil Diez S, Abbou CC, Caruelle JP, Barriatault D, Chopin DK (1992) Immunohistochemical detection of acidic fibroblast growth factor in bladder transitional cell carcinoma. Urol Res 20:211–214
Yi ES, Shabaik AS, Lacey DL, Bedoya AA, Yin S, Housley RM, Danilenko DM, Benson W, Cohen AM, Pierce GF, Thomason A, Ulrich TR (1995) Keratinocyte growth factor causes proliferation of urothelium in vivo. J Urol 154:1566–1570
Ørskov I, Ferenz A, Ørskov F (1980) Tamm-Horsfall protein or uromucoid is the normal urinary slime that traps type I fimbriatedEscherichia coli. Lancet i:887
Ertürk E, Price JM, Morris JE, Cohen S, Leith RS, Von Esch AM, Crovetti AJ (1967) The production of carcinoma of the urinary bladder in rats by feedingN-[4-(5-nitro-2-furyl)-2-thiazoly]formamide. Cancer Res 27:1998–2002
McMahon SB, Abel C (1987) A model for the study of visceral pain states: chronic inflammation of the chronic decerebrate rat urinary bladder by irritant chemicals. Pain 28:109–127
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Thulesen, J., Olsen, P.S., Grevstad, J.U. et al. A rat model with an isolated bladder in situ. Surg Today 27, 1089–1092 (1997). https://doi.org/10.1007/BF02385796
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02385796