Skip to main content
SpringerLink
Log in

Enhancing effect of thoraco-laparotomy on liver metastasis and the role played by active oxygens in its mechanism

  • Original Articles
  • Published:
Surgery Today Aims and scope Submit manuscript

Abstract

The enhancing effect on liver metastasis produced by the excessive surgical stress of thoraco-laparotomy (TL), and its regulation with a radical scavenger, were studied in 10-week-old Donryu rats. The rats were divided into three groups: those given thoraco-laparotomy for 1h (the TL group); those given laparotomy alone for 1 h (the L group); those given a short laparotomy (the C group). The effects of treatment with 5 mg/kg of EPC-K1 was assessed in the TL group. A rat hepatocellular carcinoma cell line AH 60C (5× 105 cells) was administered into the portal vein under general anesthesia. The number of metastatic liver nodules was counted 3 weeks later, and the lipid peroxide (LPO) levels of the liver and serum were measured by the TBA method on postoperative days (PODs) 1,2, and 3. The number of metastatic liver nodules was 40.6±29.7, 15.0±15.8, and 13.7±9.4 in the TL, L, and C groups, respectively. When EPC-K1 was administered to the TL group, the LPO level on POD 1 decreased from 49.8±25.8 to 18.9±7.9nM/g, and the number of metastatic liver nodules decreased from 27.2±30.0 to 8.9±12.7 in parallel. The findings of this study suggested that the excessive surgical stress produced by thoraco-laparotomy enhanced liver metastasis in parallel with an increase in LPO levels; however, the radical scavenger EPC-K1 could aid in reversing this effect.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Hattori T, Hamai Y, Ikeda H, Harada T, Ikeda T (1978) Enhancing effect of thoracotomy on tumor growth in rats. Gann (Jap J Cancer Res) 69:401–406

    CAS  Google Scholar 

  2. Toge T, Hirai T, Takiyama W, Hattori T (1981) Effect of surgical stress on natural killer activity, proliferative response of spleen cells and cytostatic activity of lung macrophages in rats. Gann (Jap J Cancer Res) 72:790–794

    CAS  Google Scholar 

  3. Hirai T (1985) Experimental studies on tumor enhancing effect of operative stress with special reference to the participation of corticosterioid (in Japanese with English abstract). Med J Hiroshima Univ 33:871–882

    CAS  Google Scholar 

  4. Babior BM, Kipnes RS, Curnutte JT (1973) Biological defense mechanisms: The production by leukocytes of superoxide, a potential bactericidal agent. J Clin Invest 52:741–744

    CAS  PubMed  Google Scholar 

  5. Weissmann G, Smolen JE, Korchak HM (1980) Release of inflammatory mediators from stimulated neutrophils N Engl J Med 303:27–34

    CAS  PubMed  Google Scholar 

  6. Iida N (1993) The effects of interlcukin-8 on the oxygen metabolism of human polyphonuclear leukocytes (in Japanese with English abstract). Med J Hiroshima Univ 41:295–304

    CAS  Google Scholar 

  7. Orr FW, Adamson IYR, Warner D, Leroyer V, Werner L, Shaughnessey S, Young L (1988) The effect of oxygen radicalmediated pulmonary endothelial damage on cancer metastasis. Mol Cell Biochem 84:189–198

    Article  CAS  PubMed  Google Scholar 

  8. Orr FW, Warner DJA (1987) Effects of neutrophil-mediated pulmonary endothelial injury on the localization and metastasis of circulating Walker carcinosarcoma cells. Invas Metast 7:183–196

    CAS  Google Scholar 

  9. Saran M, Michel C, Bors W (1990) Reaction of NO with O2−. Implications for the action of endothelium-derived relaxing factor (EDRF). Free Rad Res Comms 10:221–226

    CAS  Google Scholar 

  10. Masugi F, Nakamura T (1977) Measurement of thiobarbituric acid value in liver homogenate solubilized with sodium dodecylsulphate and variation of the values affected by vitamin E and drugs (in Japanese with English abstract). Vitamins 51:21–29

    CAS  Google Scholar 

  11. Yagi K (1975) Micro-determination of lipoperoxide in blood plasma or serum. Vitamins 49:403–405

    CAS  Google Scholar 

  12. Fisher ER, Fisher B (1965) Experimental study of factors influencing development of hepatic metastasis from circulating tumor cells. Acta Cytol 9:146–159

    Google Scholar 

  13. Adamson IYR, Young L, Orr FW (1987) Tumor metastasis after hyperoxic injury and repair of the pulmonary endothelium. Lab Invest 57:71–77

    CAS  PubMed  Google Scholar 

  14. Shaughnessy SG, Buchanan MR, Tupple S, Richardson M, Orr FW (1989) Walker carcinosarcoma cells damage endothelial cells by generation of reactive oxygen species. Am J Pathol 134:787–796

    CAS  PubMed  Google Scholar 

  15. Weiss SJ, Young J, Lobuglio AF, Slivka A, Nimen N (1981) Role of hydrogen peroxide in neutrophil-mediated destruction of cultured endothelial cells. J Clin Invest 68:714–721

    CAS  PubMed  Google Scholar 

  16. Van Zee KJ, Deforge LE, Fisher E, Marano MA, Kenny JS, Remick DG, Lowry SF, Moldawer LL (1991) IL-8 in septic shock, endotoxemia, and after IL-1 administration. J Immunol 146:3478–3482

    PubMed  Google Scholar 

  17. Sakamoto K, Arakawa H, Mita S, Ishiko T, Ikei S, Egami H, Hisano S, Ogawa M. Elevation of circulating interleukin 6 after surgery: Factors influencing the serum level. Cytokine 6:181–186

  18. Bevilacqua MP, Pober JS, Wheeler ME, Cotran RS, Gimbrone MA Jr (1985) Interleukin 1 acts on cultured human vascular endothelium to increase the adhesion of polymorphonuclear leukocytes, monocytes, and related leukocyte cell lines. J Clin Invest 76:2003–2011

    CAS  PubMed  Google Scholar 

  19. Holman JM Jr, Saba TM (1988) Hepatocyte injury during postoperative sepsis: Activated neutrophils as potential mediators. J Leukocyte Biol 43:193–203

    PubMed  Google Scholar 

  20. Sato T, Tanaka J, Kono Y, Jones RT, Cowley A, Trump BF (1982) Hepatic cellular injury following lethalEscherichia coli bacteremia in rats. Lab Invest 47:304–310

    CAS  PubMed  Google Scholar 

  21. Mukai M, Shinkai K, Tateishi R, Mori Y, Akedo H (1987) Macrophage potentiation of invasive capacity of rat ascites hepatoma cells. Cancer Res 47:2167–2171

    CAS  PubMed  Google Scholar 

  22. Larizza L, Shirrmacher V, Graf L, Pfluger E, Peres-Martinez M, Stohr M (1984) Suggestive evidence that the highly metastatic variant ESb of the T-cell lymphoma Eb is derived from spontaneous fusion with a host macrophage. Int J Cancer 34:699–707

    CAS  PubMed  Google Scholar 

  23. Glaves D (1986) Intravascular death of disseminated cancer cells mediated by superoxide anion. Invas Metast 6:101–111

    CAS  Google Scholar 

  24. Nonka Y, Iwagami H, Kimura T, Fuchimoto S, Orita K (1993) Int J Cancer 54:983–986

    Google Scholar 

  25. McCord JM, English D (1981) Superoxide dismutase: An antiinflamatory drug. In: Holcenberg JS, Roberts J (eds) Enzymes as drugs. Wiley, New York, pp 353–365

    Google Scholar 

  26. Shinkai K, Mukai M, Akedo H (1986) Superoxide radical potentiates invasive capacity of rat ascites hepatoma cells in vitro. Cancer Lett 32:7–13

    Article  CAS  PubMed  Google Scholar 

  27. Southorn PA, Powis G (1988) Free radicals in medicine. I. Chemical nature and biological reactions. Mayo Clin Proc 63:381–389

    CAS  PubMed  Google Scholar 

  28. Braugher JM, Hall ED (1989) Central nervous system trauma and stroke. I. Biochemical considerations for oxygen radical formation and lipid peroxidation. Free Rad Biol Med 6:289–301

    Google Scholar 

  29. Mori A, Edamatsu R, Kohno M, Ohmori S (1989) A new hydroxyl radical scavenger: EPC-K1. Neuroscience 15:371–376

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Surgical Oncology, Research Institute for Radiation Biology and Medicine, Hiroshima University, 1-2-3 Kasumi, Minami-ku, 734, Hiroshima, Japan

    Toshihiro Hirai, Akihiro Yoshimoto, Takashi Iwata, Yoshinori Yamashita, Masaki Kuwahara & Tetsuya Toge

Authors
  1. Toshihiro Hirai
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Akihiro Yoshimoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Takashi Iwata
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yoshinori Yamashita
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Masaki Kuwahara
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Tetsuya Toge
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Hirai, T., Yoshimoto, A., Iwata, T. et al. Enhancing effect of thoraco-laparotomy on liver metastasis and the role played by active oxygens in its mechanism. Surg Today 27, 1040–1045 (1997). https://doi.org/10.1007/BF02385785

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02385785

Key Words

Search

Navigation