Surgery Today

, Volume 27, Issue 12, pp 1144–1149 | Cite as

Characterization of certain hepatocyte-proliferating and/or protective factors induced by the sensitization of freezing-thawing hepatic tissue

  • Takahito Adachi
  • Makoto Takeuchi
  • Satomi Uno
  • Motohisa Kato
  • Kiichi Miya
  • Shigetoyo Saji
Original Articles


Although tumor cryosurgery would be expected to produce beneficial immunological effects from the enhancement of anti-tumor activity, under certain conditions the tumor may become enlarged and metastases promoted due to increased immunosuppressive activity and a high zone tolerance. In the present study, we examined whether hepatocyteproliferating factors were produced by the inoculation of freezing-thawing hepatic tissue (FTHT). Serum obtained from rats inoculated with FTHT increased DNA synthesis, according to measurement by [3H]thymidine incorporation in primary cultured rat hepatocytes. This increase was dependent on the serum concentration, with serum obtained on day 14 after the inoculation being the most potent for hepatocyte proliferation. The sensitized serum promoted DNA synthesis nearly as much as serum obtained from a 70% hepatectomized rat, but slightly less than 10ng/ml hepatocyte growth factor. The sensitized serum also protected hepatocytes from carbon tetrachloride (CCI4)-induced hepatotoxicity. Optical density measured by the 3-(4,5-dimethyl-thiazole-2-yl)-2,5-diphenyl tetrozolium bromide (MTT) cytotoxicity assay was increased, and the release of aspartate aminotransferase, alanine aminotransferase, and lactate dehydrogenase in medium was decreased by treating hepatocytes damaged by CCI4 with the sensitized serum. These results suggest that certain hepatocyte-proliferating and protective factors are induced in serum by the inoculation of freezing-thawing hepatic tissue, and that the sensitized serum may be useful in the treatment of liver failure.

Key Words

freezing-thawing hepatic tissue hepatocyte proliferating factor hepatocyte protective factor DNA synthesis carbon tetrachloride 


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Copyright information

© Springer-Verlag 1997

Authors and Affiliations

  • Takahito Adachi
    • 1
  • Makoto Takeuchi
    • 1
  • Satomi Uno
    • 1
  • Motohisa Kato
    • 1
  • Kiichi Miya
    • 1
  • Shigetoyo Saji
    • 1
  1. 1.Second Department of SurgeryGifu University School of MedicineGifuJapan

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