Summary
In vitro study of the uptake of horseradish peroxidase (HRP) by the corneal endothelium was carried out on rabbit corneae which were stored in a preserving medium for various periods. The stored corneae were incubated in HRP solution with or without adenosine triphosphate (ATP). The number of pinocytotic vesicles decreased as the storage time was prolonged. Addition of ATP to the HRP solution led to an increase in the pinocytotic vesicles in all storage periods. The pinocytosis in the 12-day stored corneal endothelium was only observed when the tissue was incubated in an HRP solution containing ATP. From these results, it was concluded that the viability of the stored cornea can be evaluated with uptake of HRP by the endothelial cell, and that the addition of ATP leads to an enhancement of pinocytotic activity in the endothelial cell.
Zusammenfassung
Die Aufnahme der Meerrettich-Peroxidase (MPO) durch das Endothel der Kaninchenhornhaut wurde in vitro untersucht. Die Hornhäute waren in einem Konservierungsmedium verschiedene Zeiten gelagert worden. Diese konservierten Hornhäute wurden in der MPO-Lösung mit oder ohne Adenosintriphosphat (ATP) inkubiert. Die Zahl der Pinocytosevesikel nahm bei Verlängerung der Lagerungszeit ab. Wurde ATP zur MPO-Lösung zugegeben nahm die Zahl der Pinocytosevesikel bei allen Lagerungszeiten zu. Bei Hornhäuten, die 12 Tage konserviert worden waren, konnte eine Pinocytose nur beobachtet werden, wenn das Gewebe in einer ATP-haltigen MPO-Lösung inkubiert worden war. Auf Grund dieser Ergebnisse kann die Lebensfähigkeit des konservierten Hornhautendothels durch die Aufnahme der Meerrettich-Peroxidase beurteilt werden.
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This investigation was supported by a grant of Stiftung Volkswagenwerk (AZ: 111516).
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Yokota, S., Waller, W.K. & Leydhecker, W. Uptake of horseradish peroxidase (HRP) by the endothelium of the short-term stored rabbit cornea. Albrecht von Graefes Arch. Klin. Ophthalmol. 199, 157–166 (1976). https://doi.org/10.1007/BF02385212
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DOI: https://doi.org/10.1007/BF02385212