Summary
Rabbit corneae were stored at a temperature of +4°C in the following media: 1. 5% Dextran T40, 2. McCoy's Medium 5a (modified), 3. Dextran T40+Mc Coy's 5a Medium + gentamicin sulphate (250 μg/ml). The endothelia of the corneae were examined by light and electron microscopy after preservation for a varied number of days. The structure of the endothelia, stored for 1, 6, 12, 18 days, was compared to fresh corneal endothelial cells. The results indicate that corneae preserved in Dextran 5% T 40 + McCoy's 5a Medium + gentamicin sulphate might be equivalent to fresh corneal tissue in keratoplasties.
Zusammenfassung
Kaninchenhornhäute wurden mit einem Sklerarand von Bulbus abpräpariert und in verschiedenen Medien bei +4°C konserviert: in 5% Dextran T 40, in McCoy's 5a Medium (modified), in 5% Dextran T 40+ McCoy's 5a Medium, in Dextran 5% T 40+ McCoy's 5a Medium + Gentamycinsulfat (250 μg/ml). Das Hornhautendothel wurde nach 1, 6, 12 und 18 Tagen licht- und elektronenmikroskopisch untersucht und verglichen mit dem Endothel frischer Kaninchenhornhäute und mit dem Hornhautendothel von Bulbi, die in feuchter Kammer bei +4°C gelagert waren.
Die Untersuchungen zeigten, daß noch eine 6-tägige Lagerung der Hornhäute in 5% Dextran T 40+ McCoy's 5a Medium mit Refobacin® gute Voraussetzungen für eine erfolgreiche Keratoplastik bietet.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literatur
Aquavella, J. V., Van Horn, D. L., Haggerty, C. J.: Corneal preservation using the M-K-Medium. Amer. J. Ophthal.80, 791 (1975)
Bigar, F., McCarey, B. E., Kaufman, H. E.: Improved corneal storage: penetrating keratoplasties in rabbits. Exp. Eve Res.20, 219 (1975)
Bito, L. Z., Salvador, E. V.: Intraocular fluid dynamics. II. Postmortem changes in solute concentrations. Exp. Eye Res.10, 273 (1970)
Capella, J. A., Kaufmann, H. E., Robbins, J. E.: Preservation of viable corneal tissue. Cryobiology2, 116 (1965)
Caulfield, J. B.: Effects of varying the vehicle for OsO4 in tissue fixation. J. biophys. biochem. Cytol.3, 827 (1957)
Dikstein, S., Maurice, D. M.: The metabolic basis to the fluid pump in the cornea. J. Physiol. (Lond.)221, 29 (1972)
Fischbarg, J.: Potential difference and fluid transport across rabbit corneal endothelium. Biochim. biophys. Acta (Amst.)228, 362 (1972)
Hodson, S.: Evidence for a bicarbonate-dependent sodium pump in corneal endothelium. Exp. Eye Res.11, 20 (1971)
Horn, D. L. Van, Schultz, R. O., DeBruin, J.: Endothelial survival in corneal tissue stored in M-K Medium. Amer. J. Ophthal.80, 642 (1975)
Imaizumi, K.: On the preservation of the cornea for penetrating keratoplasty, especially the preservation in serum. Folia ophthal. jap.19 1328 (1968)
Kuwahara, Y., Sakanoue, M., Hoyashi, M., Akija, S., Komoto, M., Kumanomido, A. Obazawa, H., Nakano, T., Hara T., Komukai, M., Saga U., Tamura, H., Ishihara, K., Shimizu, K., Takeda, K., Ooshima, T., Ozawa, H.: Studies on the long-term preservation of the cornea for penetrating keratoplasty. Acta Soc. ophthal. jap.69, 1751 (1965)
Luft, J. H.: Improvements in epoxy resin embedding methods. J. biophys. biochem. Cytol.9, 409 (1961)
McCarey, B. E., Kaufman, H. E.: Improved corneal storage. Invest. Ophthal.13, 165 (1974)
McCarey, B. E., Sakimoto, T., Bigar, F.: Ultrastructure of M-K and refrigerated moist chamber stored corneas. Invest. Ophthal.13, 859 (1974)
Mercer, E. H., Birbeck, M. S. C.: Electron Microscopy. A Handbook for Biologists. Oxford: Blackwell Scientific Publications 1966
Mishima, S., Kudo, T.: In vitro incubation of rabbit cornea. Invest. Ophthal.6, 329 (1967)
Mizukawa, T.: Recent advances in keratoplasty with special reference to the advantage of liquid preservation. Folia ophthal. jap.19, 1310 (1968)
Reynolds, E. S.: The use of lead citrate at high pH as an electronopaque stain in electron microscopy. J. Cell Biol.17, 208 (1963)
Sakimoto, T., Valent, J., Itoi, M., Kaufman, H. E.: Intermediate-term corneal storage. Invest. Ophthal.13, 219 (1974)
Stark, W. J., Maumenee, A. E., Kenyon, K. R.: Intermediate-term corneal storage for penetrating keratoplasty. Amer. J. Ophthal.79, 795 (1975)
Stocker, F. W., Levenson, D., Georgiade, N.: Medium-term preservation of corneal tissue for grafting. Arch. Ophthal.70, 554 (1963)
Trenberth, S. M., Mishima, S.: The effect of ouabain on the rabbit corneal endothelium. Invest. Ophthal.7, 44 (1968)
Waller, W. K.: Gefrierkonservierung der Hornhaut. Klin. Mbl. Augenheilk.163, 739 (1973)
Watson, M. L.: Staining of tissue sections for electron microscopy with heavy metals. J. biophys. biochem. Cytol.4, 475 (1958)
Yokota, S., Waller, W. K.: Electron microscopic localization of carbonic anhydrase (CA) activity in rabbit cornea. Albrecht v. Graefes Arch. klin. exp. Ophthal.197, 145 (1975)
Author information
Authors and Affiliations
Additional information
Mit Unterstützung der Stiftung Volkswagenwerk (AZ:111516).
Rights and permissions
About this article
Cite this article
Waller, W.K., Yokota, S. & Leydhecker, W. Licht- und elektronenmikroskopische Untersuchungen an kurzzeitkonservierten Hornhäuten. Albrecht von Graefes Arch. Klin. Ophthalmol. 199, 121–131 (1976). https://doi.org/10.1007/BF02385208
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF02385208