Molecular and Cellular Biochemistry

, Volume 110, Issue 1, pp 25–29 | Cite as

Reversible effect of calcium-binding protein regucalcin on the Ca2+-induced inhibition of deoxyuridine 5′-triphosphatase activity in rat liver cytosol

  • Masayoshi Yamaguchi
  • Takashi Sakurai


The effect of regucalcin, a calcium-binding protein isolated from rat liver cytosol, on deoxyuridine 5′-triphosphatase (dUTPase) in the cytosol of rat liver was investigated. Addition of Ca2+ up to 5.0 µM to the enzyme reaction mixture caused a significant decrease of dUTPase activity, while Zn2+, Cd2+, Co2+, Al3+, Mn2+ and Ni2+ (10 µM) did not have an appreciable effect. The Ca2+-induced decrease of dUTPase activity was reversed by the presence of regucalcin; the effect was complete at 1.0 µM of the protein. Regucalcin had no effect on the basal activity of the enzyme. Meanwhile, the reversible effect of regucalcin on the Ca2+ (10 µM)-induced decrease of dUTPase activity was not altered by the coexistence of Cd2+ or Zn2+ (10 µM). The present data suggest that liver cytosolic dUTPase is uniquely regulated by Ca2+ of various metals, and that the Ca2+ effect is reversed by regucalcin.

Key words

calcium regucalcin deoxyuridine 5′-triphosphatase rat liver cytosol 


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  1. 1.
    Reinhart PH, Taylor WM, Bygrave FL: The role of calcium ions in the mechanism of action of α-adrenergic agonists in rat liver. Biochem J 223: 1–13, 1984PubMedGoogle Scholar
  2. 2.
    Williamson JR, Cooper RH, Hoek JB: Role of calcium in the hormonal regulation of liver metabolism. Biochim Biophys Acta 639: 243–295, 1981PubMedGoogle Scholar
  3. 3.
    Yamaguchi M, Yamamoto T: Purification of calcium binding substance from soluble fraction of normal rat liver. Chem Pharm Bull 26: 1915–1918, 1978PubMedGoogle Scholar
  4. 4.
    Yamaguchi M, Sugii K: Properties of calcium-binding protein isolated from the soluble fraction of normal rat liver. Chem Pharm Bull 29: 567–570, 1981PubMedGoogle Scholar
  5. 5.
    Yamaguchi M, Mori S: Inhibitory effect of calcium-binding protein regucalcin on protein kinase C activity in rat liver cytosol. Biochem Med Metab Biol 43: 140–146, 1990PubMedCrossRefGoogle Scholar
  6. 6.
    Mori S, Yamaguchi M: Hepatic calcium-binding protein regucalcin decreases Ca2+/calmodulin-dependent protein kinase activity in rat liver cytosol. Chem Pharm Bull 38: 2216–2218, 1990PubMedGoogle Scholar
  7. 7.
    Yamaguchi M, Yoshida H: Regulatory effect of calcium-binding protein isolated from rat liver cytosol on activation of fructose 1,6-diphosphatase by Ca2+-calmodulin. Chem Pharm Bull 33: 4489–4493, 1985PubMedGoogle Scholar
  8. 8.
    Bessmans MJ, Rehman IR, Adler J, Zimmerman SB, Simms FS, Kornberg A: Enzymatic synthesis of deoxyribonucleic acid. III. The incorporation of pyridine and purine analogues into deoxyribonucleic acid. Proc Natl Acad Sci USA 44: 633–640, 1958Google Scholar
  9. 9.
    Hokari S, Sakagishi Y, Tukada K: Enhanced activity of deoxyuridine 5′-triphosphatase in regenerating rat liver. Biochem Biophys Res Commun 108: 95–101, 1982PubMedCrossRefGoogle Scholar
  10. 10.
    Nakamura M, Mori K: Colorimetric determination of inorganic phosphorus in the presence of glucose-1-phosphate and adenosin triphosphate. Nature 182: 1441–1442, 1958PubMedGoogle Scholar
  11. 11.
    Lowry OH, Rosebrough NJ, Farr AL, Randall FL: Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–273, 1951PubMedGoogle Scholar
  12. 12.
    Boynton AL, Whitefield JF, Kleine LP: Ca2+/phospholipid-dependent protein kinase activity correlates to the ability of transformed liver cells to proliferate in Ca2+-deficient medium. Biochem Biophys Res Commun 115: 383–390, 1983PubMedCrossRefGoogle Scholar
  13. 13.
    Cruise JL, Houck KA, Michalopoulos GK: Induction of DNA synthesis in cultured rat hepatocytes through stimulation of α1 adrenoreceptor by norepinephrine. Science 227: 749–751, 1985PubMedGoogle Scholar
  14. 14.
    Serratosa J, Pujol MJ, Bachs O, Carafoli E: Rearrangement of nuclear calmodulin during proliferative liver cell activation. Biochem Biophys Res Commun 150: 1162–1169, 1988PubMedCrossRefGoogle Scholar
  15. 15.
    Subramanyam C, Honn SC, Reed WC, Reddy GPV: Nuclear localization of 68 kDa calmodulin-binding protein is associated with the onset of DNA replication. J Cell Physiol 144: 423–428, 1990PubMedCrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Masayoshi Yamaguchi
    • 1
  • Takashi Sakurai
    • 1
  1. 1.Department of Environmental Biochemistry, School of Pharmaceutical SciencesUniversity of ShizuokaShizuoka CityJapan

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