Molecular and Cellular Biochemistry

, Volume 110, Issue 1, pp 25–29 | Cite as

Reversible effect of calcium-binding protein regucalcin on the Ca2+-induced inhibition of deoxyuridine 5′-triphosphatase activity in rat liver cytosol

  • Masayoshi Yamaguchi
  • Takashi Sakurai
Article

Abstract

The effect of regucalcin, a calcium-binding protein isolated from rat liver cytosol, on deoxyuridine 5′-triphosphatase (dUTPase) in the cytosol of rat liver was investigated. Addition of Ca2+ up to 5.0 µM to the enzyme reaction mixture caused a significant decrease of dUTPase activity, while Zn2+, Cd2+, Co2+, Al3+, Mn2+ and Ni2+ (10 µM) did not have an appreciable effect. The Ca2+-induced decrease of dUTPase activity was reversed by the presence of regucalcin; the effect was complete at 1.0 µM of the protein. Regucalcin had no effect on the basal activity of the enzyme. Meanwhile, the reversible effect of regucalcin on the Ca2+ (10 µM)-induced decrease of dUTPase activity was not altered by the coexistence of Cd2+ or Zn2+ (10 µM). The present data suggest that liver cytosolic dUTPase is uniquely regulated by Ca2+ of various metals, and that the Ca2+ effect is reversed by regucalcin.

Key words

calcium regucalcin deoxyuridine 5′-triphosphatase rat liver cytosol 

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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Masayoshi Yamaguchi
    • 1
  • Takashi Sakurai
    • 1
  1. 1.Department of Environmental Biochemistry, School of Pharmaceutical SciencesUniversity of ShizuokaShizuoka CityJapan

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