Abstract
Hydroxyl radicals were generated radiolytically in N2O-saturated aqueous solutions of the aminoglycoside antibiotic, gentamycin. Using the pulse radiolysis technique, the rate constant of OH radicals with gentamycin determined was 1.2·109 dm3·mol−1·s−1. Upon.OH attack a transient species with an absorption maximum at 270 nm is observed which decays by second-order kinetics within the solute concentration range of 3.2·10−5 to 1·10−3mol·dm−3. Transient species undergoes transformation to a permanent product absorbing between 260 and 340 nm with maximum absorption at 300 nm. Rate constant of the reaction of bimolecular decay of gentamycin radicals, k (Gen.+Gen.) was found to be ≈ 1.4·107 dm3·mol−1·s−1.
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P. I. Punch, D. H. Slatter, N. D. Costa, M. E. Edwards, Australian Vet. J., 62 (1985) 79.
S. Mehta, J. S. Humphrey, D. I. Schenkman, A. V. Seaber, T. P. Vail, J. Orthopaedic Res., 14 (1996) 749.
I. V. Yannas, P. L. Gordon, C. Hunag, F. H. Silver, J. F. Burke (1981) U.S. Patent 4,280,954.
R. B. Phinney, S. D. Schwartz, D. A. Lee, B. J. Mondino, Arch. Ophthalmol., 106 (1988) 1599.
A. Stemberger, K. H. Sorg, R. Ascherl, M. A. Scherer, W. Enhardt, K. Machka, R. Kotitschke, F. Lechner G. Blümel, H. Schlemmer, Polym. Mater. Sci. Eng., 59 (1988) 817.
Y.-Y. Huang, World Congress on Medical Physics and Biomedical Engineering, Nice, France, September 14–19, 1977.
K. Pietrucha, M. Lubis, Radiat. Phys. Chem., 36 (1990) 155.
K. Pietrucha, L. Góra, C. J. Doillon, Radiat. Phys. Chem., 47 (1996) 93.
P. W. Cheng, Anal. Biochem., 167 (1987) 265.
B. E. Rosenkrantz, J. R. Greco, J. G. Hoogerheide, E. M. Oden, Anal. Profiles Drug Subst., 9 (1980) 295.
S. Karolczak, K. Hodyr, R. Lubis, J. Kroh, J. Radioanal. Nucl. Chem., 101 (1986) 177.
Farhataziz, M. J. Rodgers, Radiation Chemistry. Principles and Applications, VCH Publishers, Inc, New York-Weinheim: 1987. p. 340.
G. V. Buxton, C. L. Greenstock, W. P. Helman, A. B. Ross, J. Phys. Chem. Ref. Data, 17 (1988) 513.
E. Janata, R. H. Schuler, J. Phys. Chem., 86 (1982) 2078.
G. O. Phillips, W. Griffiths, J. V. Davies, J. Chem. Soc., (1966) 194.
C. von Sonntag, The Chemical Basis of Radiation Biology, Taylor & Francis, Ltd., London—New York—Philadelphia, 1987, p. 376.
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Pietrucha, K., Góra, L. & Burczak, K. Pulse radiolytic investigation of the reaction of hydroxyl radicals with gentamycin. J Radioanal Nucl Chem 232, 83–86 (1998). https://doi.org/10.1007/BF02383718
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DOI: https://doi.org/10.1007/BF02383718