Abstract
We examined the effects of triiodothyronine (T3) on45Ca-accumulation into extracellular matrix in osteoblast-like MC3T3-El cells cultured for 7-week period, which was considered to be long enough to develop calcification. T3 increased45Ca-accumulation into sodium dodecyl sulfate-insoluble, EDTA-extractable materials cultures of MC3T3-El cells in a dose-dependent manner in the range between 10 pM and 10 nM. Contrary to the stimulatory effect on45Ca-accumulation, T3 inhibited DNA synthesis in MC3T3-El cells dose dependently between 10 pM and 10 nM. T3 stimulated the secretion of insulin-like growth factor-I (IGF-I) dose dependently between 0.1 nM and 10 nM in MC3T3-El cells cultured for 7 weeks. Antibodies to IGF-I suppressed the45Ca-accumulation induced by T3 almost to the control level. These results strongly suggest that T3 stimulates45Ca-accumulation via synthesis of IGF-I in osteoblast-like cells.
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References
Raisz LG and Kream BE. Hormonal control of skeletal growth. Annu Rev Physiol. 43: 225–238, 1981
Nijweide PJ, Burger EH and Feyen JHM. Cells of bone: proliferation, differentiation and hormonal regulation. Physiol Rev. 66: 855–886, 1986
Rizzoli R, Poser J and Burgi U. Nuclear thyroid hormone receptor in cultured bone cells. Metabolism 35: 71–74, 1986
Kasano K, Sato K, Han DC, Fujii Y, Tsushima T and Shizume K. Stimulation of alkaline phosphatase activity by thyroid hormone in mouse osteoblastlike cells (MC3T3-El); a possible mechanism of hyper-alkaline phosphatasis in hyperthyroidism. Bone Miner. 4: 355–363, 1988
Kodama H, Amagai Y, Sudo H, Kasai S and Yamamoto S. Establishment of a clonal osteogenic cell line from newborn mouse calvaria. Jan J Oral Biol. 23: 899–901, 1981
Sudo H, Kodama H, Amagai Y, Yamamoto S and Kasai S. In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria. J Cell Biol. 96: 191–198, 1983
Mundy GR, Shapiro JL, Bandelin JG, Canalis EM and Raisz LG. Direct stimulation of bone resorption by thyroid hormones. J Clin Invest. 58: 529–534, 1976
Klaushofer K, Hoffman O, Gleispach H, Leis HJ, Czerwenka E, Koller K and Peterlik M. Bone-resorbing activity of thyroid hormones is related to prostaglandin production in cultured neonatal mouse calvaria. J Bone Miner Res. 4: 305–312, 1989
Ernst M and Froesch ER. Triiodothyronine stimulates proliferation of osteoblast-like cells in serumfree culture. FEBS Lett. 220: 163–166, 1987
Sato K, Han DC, Fujii Y, Tsushima T and Shizume K. Thyroid hormone stimulates alkaline phosphatase activity in cultured rat osteoblastic cells (ROS17/2.8) through 3,5,3′-triiodo-L-thyronine nuclear receptors. Endocrinology 120: 1873–1881, 1987
Doty SB and Schofield BH. Enzyme histochemistry of bone and cartilage cells. Prog Histochem Cytochem. 8: 1–14, 1976
Canalis E. Effect of insulin-like growth factor I on DNA and protein synthesis in cultured rat calvaria. J Clin Invest. 66: 709–719, 1980
Schmid C, Steiner T and Froesch ER. Insulin-like growth factors stimulate synthesis of nucleic acids and glycogen in cultured calvaria cells. Calcif Tissue Int. 35: 578–585, 1983
Canalis E, McCarthy T and Centrella M. Isolation and characterization of insulin-like growth factor I (somatomedin-C) from cultures of fetal rat calvariae. Endocrinology 122: 22–27, 1988
Kurose H, Yamaoka K, Okada S, Nakajima S and Seino Y. 1,25-Dihydroxyvitamin D3 [1,25-(OH)2D3] increases insulin-like growth factor I (IGF-I) receptors in clonal osteoblastic cells. study on interaction of IGF-I and 1, 25-(OH)2D3. Endocrinology 126: 2088–2094, 1990
Kozawa O, Takatsuki K, Kotake K, Yoneda M, Oiso Y and Saito H. Possible involvement of protein kinase C in proliferation and differentiation of osteoblast-like cells. FEBS Lett. 243: 183–185, 1989
Kozawa O, Takatsuki K, Tokuda H, Miwa M, Kotake K, Yoneda M, Oiso Y and Saito H. Insulin-like growth factor-I stimulates45Ca-accumulation in cultures of osteoblast-like cells. J Bone Miner Met. 8: 161–164, 1990
Furlanetto RW, Underwood LE, Van Wyk JJ and D'Ercole AJ Estimation of somatomedin-C levels in normals and patients with pituitary disease by radioimmunoassay. J Clin Invest. 60: 648–657, 1977
Warshawsky H and Moor G. A technique for the fixation and decalcification of rat incisors for electron microscopy. J Histochem Cytochem. 15: 542–549, 1967
Klapper DG, Svoboda ME and Van Wyk JJ. Sequence analysis of somatomedin-C: confirmation of identity with insulin-like growth factor I. Endocrinology 112: 2215–2217, 1983
Kotoyori J, Kozawa O, Tokuda H, Miwa M and Oiso Y. Cyclic AMP stimulates45Ca-accumulation via synthesis of insulin-like gronth factor-I in cultures of osteoblast-like cells. J Bone Miner Met. 10: 135–139, 1992
Kusugami K, Youngman KR, West GA and Fiocchi C. Intestinal immune reactivity to interleukin 2 differs among Crohn's disease, ulcerative colitis and controls. Gastroenterology 97: 1–9, 1989
Dicker P and Rozengurt E. Phorbol esters and vasopressin stimulate DNA synthesis by a common mechanism. Nature 287: 607–612, 1980
Daughaday WH, Mariz IK and Blethen SL. Inhibition of access of bound somatomedin to membrane receptor and immunobinding sites: a comparison of radioreceptor and radioimmunoassay of somatomedin in native and acid-ethanol-extracted serum. J Clin Endocrinol Metab. 51: 781–788, 1980
Froesch ER, Schmid CH, Schwander J and Zapf J. Actions of insulin-like growth factors. Ann Rev Physiol. 47: 443–467, 1985
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Kozawa, O., Tokuda, H., Miwa, M. et al. Triiodothyronine stimulates45Ca-accumulation via synthesis of insulin-like growth factor-I in cultures of osteoblast-like cells. J Bone Miner Metab 11, 1–6 (1993). https://doi.org/10.1007/BF02383527
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DOI: https://doi.org/10.1007/BF02383527