Journal of Bone and Mineral Metabolism

, Volume 10, Issue 1, pp 26–30 | Cite as

Effect of microfibrillar collagen on growth and differentiation of osteoblast-like MC3T3-E1 cells

  • Shigeyuki Tsukagoshi
  • Toshio Matsumoto
  • Shun-ici Harada
  • Etsuro Ogata
Original Articles

Abstract

Effect of microfibrillar collagen on the proliferation and differentiation of osteoblastic cells was studied using MC3T3-E1 (E1) cells. In order to achieve direct contact of microfibrillar collagen with E1 cells, they were embedded in denatured collagen gel, and DNA content, [3H] thymidine incorporation, alkaline phosphatase activity, and45Ca accumulation were examined after long-term culture. Microfibrillar collagen embedded with E1 cells increased DNA content and stimulated DNA synthesis of E1 cells in a dose-dependent manner. In vitro mineralization induced by E1 cells was also stimulated by microfibrillar collagen in a dose-dependent manner: 1mg/ml of microfibrillar collagen stimulated45Ca accumulation by about 3 fold, and 10 mg/ml by 5 fold. Alkaline phosphatase activity was not affected by the presence of microfibrillar collagen. Because the interaction of specific RGD tripeptide recognition site on collagen fiber with cell surface adhesion receptors is proposed to affect the proliferation and differentiation of various cells, it is suggested that the interaction of osteoblastic cells with collagen fibers plays an important role in the regulation of proliferation and the expression of osteoblastic phenotype in these cells.

Key words

microfibrillar collagen osteoblasts MC3T3-E1 cells cell proliferation mineralization 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Vlodavsky, I., Lui, G.M. and Gospodarowicz, D.: Morphological appearance, growth behavior and migratory activity of human tumor cells maintained on extracellular matrix versus plastic. Cell 19: 607–616, 1980.CrossRefPubMedGoogle Scholar
  2. 2.
    Gospodarowicz, D., Delgado, D. and Vlodavsky, I.: Permissive effect of the extracellular matrix on cell prolilferation in vitro. Proc Natl Acad Sci USA 77: 4094–4098, 1980.PubMedGoogle Scholar
  3. 3.
    Buck, C.A. and Horowitz, A.F.: Cell surface receptors for extracellular matrix molecules. Ann Rev Cell Biol 3: 179–205, 1987.PubMedGoogle Scholar
  4. 4.
    Ruoslahti, E. and Pierschbacher, M.D.: New perspectives in cell adhesion: RGD and integrins. Science 238: 491–497, 1987.PubMedGoogle Scholar
  5. 5.
    Hynes, R.O.: Integrins: A family of cell surface receptors. Cell 48: 549–554, 1987.PubMedGoogle Scholar
  6. 6.
    Horton, M.A. and Davies, J.: Adhesion receptors in bone. J Bone Mineral Res 4: 803–808, 1989.Google Scholar
  7. 7.
    Harada, S., Matsumoto, T. and Ogata, E.: Role of ascorbic acid in the regulation of proliferation in osteoblast-like MC3T3-E1 cells. J Bone Mineral Res 6: 903–908, 1991.Google Scholar
  8. 8.
    Tajima, S. and Nagai, Y.: Isolation and partial characterization of collagen fibrils, fibers and fiber-bundles from insoluble calf dermis. Connect Tissue Res 7: 157–163, 1980.PubMedGoogle Scholar
  9. 9.
    Matsumoto, T., Igarashi, C., Takeuchi, Y., Harada, S., Kikuchi, T., Yamato, H. and Ogata, E.: Stimulation by 1,25-dihydroxyvitamin D3 of in vitro mineralization induced by ostcoblast-like MC3T3-E1 cells. Bone 12: 27–32, 1991.CrossRefPubMedGoogle Scholar
  10. 10.
    Labarca, C. and Paigen, K.A.: A simple, rapid and sensitive DNA assay procedure. Anal Biochem 102: 344–352, 1980.CrossRefPubMedGoogle Scholar
  11. 11.
    Ikeda, K., Matsumoto, T., Morita, K., Kurokawa, K. and Ogata, E.: Inhibition of in vitro mineralization by aluminum in a clonal osteoblast-like cell line, MC3T3-E1. Calcif Tissue Int 39: 319–323, 1986.PubMedGoogle Scholar
  12. 12.
    Kodama, H., Amagai, Y., Sudo, H., Kasai, S. and Yamamoto, S.: Establishment of a clonal osteogenic cell line from newborn mouse calvaria. Jpn J Oral Biol 23: 899–901, 1981.Google Scholar
  13. 13.
    Sudo, H., Kodama, H., Amagai, Y., Yamamoto, S. and Kasai, S.: In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria. J Cell Biol 96: 191–198, 1983.CrossRefPubMedGoogle Scholar

Copyright information

© Japanese Society of Bone Metabolism Research 1992

Authors and Affiliations

  • Shigeyuki Tsukagoshi
    • 1
  • Toshio Matsumoto
    • 1
  • Shun-ici Harada
    • 1
  • Etsuro Ogata
    • 1
  1. 1.Fourth Department of Internal MedicineUniversity of Tokyo School of MedicineTokyoJapan

Personalised recommendations