Journal of Bone and Mineral Metabolism

, Volume 10, Issue 1, pp 26–30 | Cite as

Effect of microfibrillar collagen on growth and differentiation of osteoblast-like MC3T3-E1 cells

  • Shigeyuki Tsukagoshi
  • Toshio Matsumoto
  • Shun-ici Harada
  • Etsuro Ogata
Original Articles


Effect of microfibrillar collagen on the proliferation and differentiation of osteoblastic cells was studied using MC3T3-E1 (E1) cells. In order to achieve direct contact of microfibrillar collagen with E1 cells, they were embedded in denatured collagen gel, and DNA content, [3H] thymidine incorporation, alkaline phosphatase activity, and45Ca accumulation were examined after long-term culture. Microfibrillar collagen embedded with E1 cells increased DNA content and stimulated DNA synthesis of E1 cells in a dose-dependent manner. In vitro mineralization induced by E1 cells was also stimulated by microfibrillar collagen in a dose-dependent manner: 1mg/ml of microfibrillar collagen stimulated45Ca accumulation by about 3 fold, and 10 mg/ml by 5 fold. Alkaline phosphatase activity was not affected by the presence of microfibrillar collagen. Because the interaction of specific RGD tripeptide recognition site on collagen fiber with cell surface adhesion receptors is proposed to affect the proliferation and differentiation of various cells, it is suggested that the interaction of osteoblastic cells with collagen fibers plays an important role in the regulation of proliferation and the expression of osteoblastic phenotype in these cells.

Key words

microfibrillar collagen osteoblasts MC3T3-E1 cells cell proliferation mineralization 


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Copyright information

© Japanese Society of Bone Metabolism Research 1992

Authors and Affiliations

  • Shigeyuki Tsukagoshi
    • 1
  • Toshio Matsumoto
    • 1
  • Shun-ici Harada
    • 1
  • Etsuro Ogata
    • 1
  1. 1.Fourth Department of Internal MedicineUniversity of Tokyo School of MedicineTokyoJapan

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