Tropical Animal Health and Production

, Volume 8, Issue 1, pp 97–101 | Cite as

Application of the indirect fluorescent antibody test for diagnosis ofTheileria hirci infection of sheep: Using cell culture schizont antigen

  • N. Hawa
  • B. M. A. Latif
  • F. A. Bakir
Article

Summary

The indirect fluorescent antibody test was applied for detection of circulating antibodies in sheep as a result ofTheileria hirci infections. A schizont antigen was prepared from anin vitro culture suspension of lymphoid cells infected withT. hirci macroschizonts. The peak antibody titre of1/8,192 was reached24 days after the initial antibody rise in the sheep experimentally infected by means ofHyalomma anatolicum anatolicum ticks.

Keywords

Public Health Cell Culture Culture Suspension Antibody Titre Veterinary Medicine 

Résumé

Le test de la recherche des anticorps par immunofluorescence indirecte a été appliqué à la détection des anticorps circulants de mouton des aux infections parTh. hirci. Un antigène a été préparé à partir d'une suspensionin vitro de cellules lymphoïdes infectées par des macroschizontes deTh. hirci. Le taux le plus élevé en anticorps (1/8192) a été atteint 24 jours après la première sortie d'anticorps chez le mouton infecté expérimentalement par des tiques du genreHyalomma anatolicum anatolicum.

Resumen

Se utilizó la prueba indirecta de anticuerpos fluorescentes para la detección de anticuerpos circulantes en ovejas infectadas conTheileria hirci. Se preparó un antígeno de esquizontes del cultivoin vitro de una suspensión de células linfoides infectadas con macroesquizontes deT. hirci. El nivel más alto de anticuerpos 1/8192, se obtuvo 24 días después de la respuesta inmunitaria inicial en la oveja infectada experimentalmente utilizando como vectores garrapatasHyalomma anatolicum anatocilum.

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Copyright information

© E. & S. Livingstone Ltd 1976

Authors and Affiliations

  • N. Hawa
    • 1
  • B. M. A. Latif
    • 2
  • F. A. Bakir
    • 3
  1. 1.Veterinary Laboratories and Research InstituteAbu-GhraibIraq
  2. 2.College of Veterinary MedicineAl-AmeriaIraq
  3. 3.Armour Pharmaceutical Co. Ltd.EastbourneEngland

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