, Volume 33, Issue 2, pp 227–238 | Cite as

Protein variation, taxonomy and differentiation in five species of marmosets (genusCallithrixErxleben, 1777)

  • C. M. M. Meireles
  • M. I. C. Sampaio
  • H. Schneider
  • M. P. C. Schneider


The electrophoretic patterns of 15 protein systems codified for 20 genetic loci were investigated using horizontal electrophoresis. A total of 150 blood samples, from five species of the genusCallithrix were analyzed. Polymorphic variation was observed in 10 out 20 loci analyzed. The genotypic distributions are in Hardy-Weinberg equilibrium. The average heterozygosity (H) varied from 1% to 5%, similar to those observed for other Neotropical primates. The genetic distance coefficients revealed a phylogenetic separation of these species into two groups: (1) “argentata” (C. humeralifer andC. emiliae); (2) “jacchus” (C. jacchus, C. penicillata, andC. geoffroyi). This arrangement is according to the taxonomic arrangement proposed byHershkovitz (1977),de Vivo (1988), andMittermeier et al. (1988). The results in each group are compatible with the subspecies values recorded for the Platyrrhini. These values showed that:C. humeralifer andC. emiliae are subspecies ofC. argentata;C. jacchus, C. penicillata, andC. geoffroyi are subspecies ofC. jacchus. These results also suggest thatC. j. geoffroyi is the “jacchus” group taxon, most similar genetically to the “argentata” group.

Key Words

Callithrix Heterozygosity Biochemical markers Taxonomy Polymorphism Evolution 


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  1. Ávila-pires, F. D. de, 1969. Taxonomia e zoogeografia do gêneroCallithrix Erxleben, 1777 (Primates, Callithricidae).Rev. Brasil. Biol., 29: 49–64.Google Scholar
  2. ————, 1986. On the validity and geographical distribution ofCallithrix argentata emiliae Thomas, 1920 (Primates, Callithrichidae). In:A Primatologia no Brasil, Vol. 2,M. T. de Mello (ed.), Sociedade Brasileira de Primatologia, Brasília-DF, pp. 319–322.Google Scholar
  3. Coimbra-Filho, A. F., 1984. Situação atual dos calitriquídeos que ocorrem no Brasil (Callitrichidae, Primates). In:A Primatologia no Brasil, Vol. 1,M. T. de Mello (ed.), Sociedade Brasileira de Primatologia, Brasília-DF, pp. 15–33.Google Scholar
  4. Cruz Lima, E. da, 1944.Mamíferos da Amazônia. Introdução Geral e Primatas. Vol. 1. Contr. Mus. Paraense Emílio Goeldi de História Natural e Etnografia, Belém, Pará.Google Scholar
  5. de Vivo, M., 1985. On some monkeys from Rondônia Brasil (Primates: Callitrichidae, Cebidae).Papéis Avulsos de Zoologia, 36: 103–110.Google Scholar
  6. ————, 1988. Sistemática deCallithrix Erxleben, 1777 (Callitrichidae, Primates). Unpublished Ph.D. thesis, Univ. de São Paulo, São Paulo.Google Scholar
  7. Dietz, E. J., 1983. Permutation tests for association between two distance matrices.Systematic Zoology, 32: 21–26.Google Scholar
  8. Ferrari, S. F. & M. A. Lopes, 1992. A new species of marmoset, genusCallithrix Erxleben, 1777 (Callitrichidae, Primates) from western Brazilian Amazonia,Goeldiana, Zoologia, 7: (in press).Google Scholar
  9. Forman, L., D. G. Kleiman, R. M. Bush, J. M. Dietz, J. D. Ballou, L. G. Phillips, A. F. Coimbra-filho, &S. J. O'brien, 1986. Genetic variation within and among lion tamarins.Amer. J. Phys. Anthropol., 71: 1–11.CrossRefGoogle Scholar
  10. Franco, M. H. L. P. &F. M. Salzano, 1985. A comparative study of albumin variants found in Brazil.Human Hered., 35: 34–38.Google Scholar
  11. ————, ————, &F. A. M. Lima, 1981. Blood groups and serum protein types in two brazilian populations.Rev. Brasil. Genet., 4: 689–704.Google Scholar
  12. Harris, H. &D. A. Hopkinson, 1976.Handbook of Enzyme Electrophoresis in Human Genetics. North-Holland, Amsterdam.Google Scholar
  13. Hershkovitz, P., 1968. Metachromism on the principle of evolutionary changes in mammalian tegumentary colors.Evolution, 22: 556–575.Google Scholar
  14. ————, 1975. Comments on taxonomy of Brazilian marmosets (Callithrix, Callitrichidae).Folia Primatol., 24: 137–172.PubMedGoogle Scholar
  15. ————, 1977.Living New World Monkeys (Platyrrhini) with an Introduction to Primates. Vol. 1. Univ. of Chicago Press, Chicago.Google Scholar
  16. Kinzey, W. G., 1982. Distribution of primates and forest refuges. In:Biological Diversification in the Tropics, G. T. Prance (ed.), Columbia Univ. Press, New York, pp. 455–482.Google Scholar
  17. Lima, M. M. C. de, M. I. C. Sampaio, M. P. C. Schneider, W. Scheffrahn, H. Schneider, &F. M. Salzano, 1990. Chromosome and protein variation in red howler monkeys.Rev. Brasil. Genet., 13: 789–802.Google Scholar
  18. Mantel, N., 1967. The detection of disease clustering and a generalize regression approach.Cancer Res., 27: 209–220.PubMedGoogle Scholar
  19. Melo, A. C. A., 1990. Variabilidade Protéica e Distância Genética em Duas Espécies do GêneroSaguinus (Callitrichidae — Primates — Platyrrhini). Unpublished M.Sc. thesis, Univ. Federal do Pará, Belém.Google Scholar
  20. Mittermeier, R. A., A. B. Rylands, &A. F. Coimbra-filho, 1988. Systematics: species and subspecies — an update. In:Ecology and Behavior of Neotropical Primates, Vol. 2,R. A. Mittermeier,A. B. Rylands,A. F. Coimbra-Filho, &G. A. B. da Fonseca (eds.), World Wildlife Fund., Washington D. C., pp. 13–75.Google Scholar
  21. Nei, M., 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals.Genetics, 89: 583–590.Google Scholar
  22. ————, 1987.Molecular Evolutionary Genetics. Columbia Univ. Press, New York.Google Scholar
  23. Reed, T. E. &W. J. Schull, 1968. A general maximum likelihood estimation program.Amer. J. Human Genet., 20: 579–580.Google Scholar
  24. Rohlf, J. F., 1982.BIOM: a Package of Statistical Programs. State Univ. of New York, New York.Google Scholar
  25. Rosenberger, A. L., 1984. Aspects of the systematics and evolution of the marmosets. In:A Primatologia no Brasil, Vol. 1,M. T. de Mello (ed.), Sociedade Brasileira de Primatologia, Brasília-DF, pp. 159–180.Google Scholar
  26. Schneider, M. P. C., 1988. Variação Protéica em Primatas da Amazônia e seu Significado Evolutivo. Unpublished Ph.D. thesis, Univ. Federal do Rio Grande do Sul, Porto Alegre.Google Scholar
  27. Silva, B. T. F. da, 1990. Variabilidade Protéica e Diferenciação Evolutiva no GêneroSaimiri (Cebidae — Primates). Unpublished M.Sc. thesis, Univ. Federal do Rio Grande do Sul, Porto Alegre.Google Scholar
  28. Sussman, R. W. &W. G. Kinzey, 1984. The ecological role of the Callitrichidae: a review. Amer.J. Phys. Anthropol., 64: 419–449.Google Scholar
  29. Swofford, D. L. & R. B. Selander, 1981.Biosys-1. A Computer Program for Analysis Variation in Genetics. Users Manual, Dept. of Genetics and Development, Univ. Illinois at Urbana-Champaing (unpubl. manual).Google Scholar
  30. Thorpe, J. P., 1982. The molecular clock hypothesis: biochemical evolution, genetic differentiation and systematics.Ann. Rev. Ecol. Syst., 13: 139–168.CrossRefGoogle Scholar

Copyright information

© Japan Monkey Centre 1992

Authors and Affiliations

  • C. M. M. Meireles
    • 1
  • M. I. C. Sampaio
    • 1
  • H. Schneider
    • 1
  • M. P. C. Schneider
    • 1
  1. 1.Departamento de Genética, Centro de Ciências BiológicasCampus Universitário do Guamá, Universidade Federal do Pará, CEP 66.075Belém, ParáBrasil

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