Advertisement

Primates

, Volume 27, Issue 2, pp 259–268 | Cite as

Fetal hemoglobin distributions among adult baboon and macaque species

  • Robin Scott
  • Stephen Anyaibe
  • Anthony Yancey
  • Verle Headings
Article

Abstract

Heritable variation in fetal hemoglobin (Hb F) in erythrocytes of the adult human has been shown to occur at more than one genetic locus. Heritable variation has also been reported in adult baboons. Nonhuman primates thus may serve as useful models for understanding how Hb F is regulated in the human. In the study reported here we identified Hb F in hemolysates from 27 of 32 rhesus macaques, from 32 of 32 baboons, and from none of 35 cynomoglus macaques. Hb F as a percentage of total hemoglobin occurred as a normally distributed variable among rhesus macaques but among baboons the distribution was both skewed and kurtotic. Such difference could be either a consequence of nonrandom sampling of the gene pool in one of the species, or a consequence of species evolution. A technique of single cell hemoglobin electrophoresis was applied to erythrocytes from three adult pig-tailed macaques. This demonstrated that erythrocytes which contain Hb F (F-cells) also customarily contain Hb A and that the proportions of these two hemoglobins varies substantially among the F-cells, as we previously noted for human F-cells. We conclude that the macaques could serve as useful models for understanding Hb F regulation in the human.

Key Words

Nonhuman primates Baboons Macaques Fetal hemoglobin F-cells Hemoglobin electrophoresis 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Anyaibe, S. &V. Headings, 1977. Identification of hemoglobins in single erythrocytes by electrophoresis.Amer. J. Hematol., 32: 307–312.Google Scholar
  2. Basch, R. S., 1972. Hemoglobin polymorphism in the rhesus macaque.Nature, 238: 238–240.Google Scholar
  3. Bhattacharya, S. P., S. I. O. Anyaibe, &V. E. Headings, 1976. Biological variations in the heterogeneous distribution of hemoglobin F among erythrocytes.Brit. J. Haematol., 33: 401–408.Google Scholar
  4. Boyer, S. H., T. K. Belding, L. Margolet, &A. N. Noyes, 1975. Fetal hemoglobin restriction to a few erythrocytes (F-cells) in normal human adults.Science, 188: 361–363.PubMedGoogle Scholar
  5. Buettner-Janusch, J. &V. Buettner-Janusch, 1968. Fetal hemoglobin in newborn baboons,Papio cynocephalus. Amer. J. Phys. Anthropol., 28: 101–104.Google Scholar
  6. ———— & ————, 1970. Hemoglobins of baboons,Papio cynocephalus, P. gelada, andP. hamadryas.Amer. J. Phys. Anthropol., 33: 73–82.Google Scholar
  7. DeSimone, J., P. Heller, J. Amsel, &M. Usman, 1980. Magnitude of the fetal hemoglobin response to acute hemolytic anemia in baboons is controlled by genetic factors.J. Clin. Invest., 65: 224–226.PubMedGoogle Scholar
  8. ————, ————,M. Biel, &D. Zwiers, 1981. Genetic relationship between fetal Hb levels in normal and erythropoietically stressed baboons.Brit. J. Haematol., 49: 175–183.Google Scholar
  9. Dover, G. J. &S. H. Boyer, 1980. Quantitation of hemoglobin within individual red cells. Asynchronous biosynthesis of fetal and adult hemoglobin during erythroid maturation in normal subjects.Blood, 56: 1082–1091.PubMedGoogle Scholar
  10. ————, ————, &M. E. Pembrey, 1981. F-cell production in sickle cell anemia: Regulation by genes linked toβ-hemoglobin locus.Science, 211: 1441–1444.PubMedGoogle Scholar
  11. Mahoney, W. C. &P. E. Nute, 1980. Fetal hemoglobin of rhesus monkeyMacaca mulatta: Complete primary structure of γ chain.Biochemistry, 19: 4436–4442.PubMedGoogle Scholar
  12. Nute, P. E. &W. C. Mahoney, 1980. Complete primary structure of theβ chain from the hemoglobin of a baboon,Papio cynocephalus.Hemoglobin, 4: 109–123.PubMedGoogle Scholar
  13. Shepard, M. K., D. J. Weatherall, &C. L. Conley, 1962. Semiquantitative estimation of the distribution of fetal hemoglobin in red cell populations.Bull. Johns Hopkins Hospital, 110: 293–310.Google Scholar
  14. Sokal, R. R. &F. J. Rohlf, 1969.Biometry: The Principles and Practice of Statistics in Biological Research. W. H. Freeman & Co., San Francisco.Google Scholar
  15. Wood, W. G., G. Stamatoyannopoulos, G. Lim, &P. E. Nute, 1975. F-cells in the adult: Normal values and levels in individuals with hereditary and acquired elevations of Hb F.Blood, 46: 671–682.PubMedGoogle Scholar
  16. Wu, N. C., D. A. Sikkema, R. M. Zucker, &N. H. Altman, 1977a. Studies of rhesus monkey (Macaca mulatta) hemoglobin.Lab. Anim. Sci., 27: 65–68.PubMedGoogle Scholar
  17. ————, ————,N. H. Altman, &R. M. Zucker, 1977b. Identification of hemoglobin within individual rhesus monkey (Macaca mulatta) erythrocytes.Lab. Anim. Sci., 27: 986–992.PubMedGoogle Scholar
  18. Zago, M. A., W. G. Wood, J. B. Clegg, D. J. Weatherall, M. O'Sullivan, &H. Gunson, 1979. Genetic control of F-cells in human adults.Blood, 53: 977–986.PubMedGoogle Scholar

Copyright information

© Japan Monkey Centre 1986

Authors and Affiliations

  • Robin Scott
    • 1
  • Stephen Anyaibe
    • 1
  • Anthony Yancey
    • 1
    • 2
  • Verle Headings
    • 1
  1. 1.Department of Genetics, Graduate School of Arts and Sciences, Division of Medical Genetics, Department of Fediatrics and Child Health, College of MedicineHoward UniversityWashington, D.C.U.S.A.
  2. 2.Walter Reed Army Medical CenterWashington, D.C.U.S.A.

Personalised recommendations