Primates

, Volume 27, Issue 4, pp 493–506 | Cite as

Concentrations of steroids in the utero-ovarian vein blood, serially collected from the two sides of individual baboons, during the follicullar phase

  • A. A. Shaikh
  • E. E. Gbur
  • S. A. Shaikh
Article
  • 18 Downloads

Abstract

The purpose of this study was to determine and compare the follicular phase steroid hormone secretion into the utero-ovarian vein by the ovary with a dominant follicle and the contralateral ovary in the same baboon. Serial utero-ovarian vein blood from both sides was collected in 25 baboons by the use of a laparoscope on alternate days, starting on day 1 or 3 of the cycle and continuing through 2 to 3 days post-ovulation. Approximately 3–4 days before the day of expected ovulation, samples were collected at 8-hr intervals. Steroids estradiol (E2) and progesterone (P) were measured in all utero-ovarian vein plasma by radioimmunoassay. In the peripheral plasma, E2, P, LH, and FSH measurements were carried out. Concentrations of steroids were significantly higher on the side of the ovulating ovary by day 5 before ovulation. Individual plots however, indicated that some baboons may establish the dominant side as early as day 11 before ovulation. The preovulatory gonadotropins had a differential effect on the two ovaries. For example, E2 values on the ovulatory side ovary declined after increases in LH/FSH, whereas on the contralateral side these values had increased. Both sides showed increases in the level of P with the increases in LH. The mean interval from E2 peak to LH peak was 24 hrs and LH peak to ovulation was 24 hrs.

Key Words

Baboons Ovary Steroids Gonadotropins Follicular phase 

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References

  1. Armstrong, D. T., T. J. Weiss, G. Selstam, &R. F. Seamark, 1981. Follicular steroid biosynthesis: hormonal and cellular interactions.J. Reprod. Fertil. Suppl., 30: 143–154.PubMedGoogle Scholar
  2. Baird, D. T., 1978. Pulsatile secretion of LH and ovarian estradiol during the follicular phase of the sheep estrous cycle.Biol. Reprod., 18: 359–364.CrossRefPubMedGoogle Scholar
  3. ———— &L. S. Fraser, 1974. Blood production and ovarian secretion rates of estradiol-17β and estrone in women throughout the menstrual cycle.J. Clin. Endocrinol. Metab., 38: 1009–1017.PubMedGoogle Scholar
  4. Butcher, R. L., W. E. Collins, &N. W. Fugo, 1974. Plasma concentration of LH, FSH, prolactin, progesterone, and estradiol-17β throughout the 4-day estrous cycle of the rat.Endocrinology, 94: 1704–1708.PubMedGoogle Scholar
  5. Castracane, V. D., G. T. Moore, &A. A. Shaikh, 1979. Ovarian function in hysterectomizedMacaca fascicularis.Biol. Reprod., 20: 462–472.CrossRefPubMedGoogle Scholar
  6. Channing, C. P., 1969. Steroidogenesis and morphology of human ovarian cell types in tissue culture.J. Clin. Endocrinol. Metab., 45: 297.Google Scholar
  7. ———— &S. P. Coudert, 1976. Contribution of granulose cells and follicular fluid to ovarian estrogen secretion in the rhesus monkey in vivo.Endocrinology, 98: 590–593.PubMedGoogle Scholar
  8. Daniel, W. W., 1978. Making inferences about a location parameter. In:Applied Nonparametric Statistics,W. W. Danile (ed.), Houghton Mifflin Co., Boston, pp. 26–31.Google Scholar
  9. Dizerega, G. S., E. L. Marut, C. K. Turner, &G. D. Hodgen, 1980. Asymmetrical ovarian function during recruitment and selection of the dominant follicle in the menstrual cycle of the rhesus monkey.J. Clin. Endocrinol. Metab., 51: 698–701.PubMedGoogle Scholar
  10. Feder, H. H., K. Brown-Grant, &C. S. Corker, 1971. Preovulatory progesterone, the adrenal cortex and the critical period for luteinizing hormone release in rats.J. Endocrinol., 50: 29–39.PubMedGoogle Scholar
  11. Gemzel, C. A., 1962. Induction of ovulation with human pituitary gonadotrophins.Fertil. Steril., 13: 153.Google Scholar
  12. Goncharov, N., T. Aso, Z. Cekan, N. Pachalia, &E. Diczfalusy, 1976. Hormonal changes during the menstrual cycle of the baboon (Papio hamadryas).Acta Endocrinol., 82: 396–411.PubMedGoogle Scholar
  13. Goodman, A. L., C. D. Descalzi, D. K. Johnson, &G. D. Hodgen, 1977. Composite pattern of circulating LH, FSH, estradiol and progesterone during the menstrual cycle in cynomolgus monkeys.Proc. Soc. Exp. Biol. Med., 155: 479–481.PubMedGoogle Scholar
  14. Greenwood, F. S., W. M. Hunter, &J. S. Glover, 1963. The preparation of131I-labelled human growth hormone of high specific radio-activity.Biochem. J., 89: 114–123.PubMedGoogle Scholar
  15. Hamberger, L., T. Hillensio, &J. Ahren, 1978. Steroidogenesis in isolated cells of preovulatory rat follicles.Endocrinology, 103: 771–777.PubMedGoogle Scholar
  16. Hodgen, G. D., J. W. Wilks, J. L. Vaitukaitis, H. C. Chen, H. Papkoff, &G. T. Ross, 1976. A new radio-immunoassay for follicle stimulating hormone in macaques: Ovulatory menstrual cycles.Endocrinology, 99: 137–145.PubMedGoogle Scholar
  17. Hotchkiss, J., L. E. Atkinson, &E. Knobil, 1971. Time course of serum estrogen and luteinizing hormone (LH) concentrations during the menstrual cycle of the rhesus monkeys.Endocrinology, 89: 171–183.Google Scholar
  18. Jha, P., A. K. Jain, S. H. Rehman, T. G. Dugwekar, &K. R. Laumas, 1978. Serum LH, FSH, progesterone and estradiol-17β levels of normally menstruating Indian women.Ind. J. Med. Res., 67: 57–65.Google Scholar
  19. Katz, V. &D. T. Armstrong, 1976. Inhibition of ovarian estradiol-17β secretion by luteinizing hormone in prepubertal, pregnant mare serum treated rats.Endocrinology, 99: 1442–1447.PubMedGoogle Scholar
  20. Kletzky, O. A., R. M. Nakamura, I. H. Thorneycroft, &D. R. Mishell, Jr., 1975. Log normal distribution of gonadotropins and ovarian steroid values in the normal menstrual cycle.Amer. J. Obstet. Gynecol., 121: 688–694.Google Scholar
  21. Knobil, E., 1974. On the control of gonadotropin secretion in the rhesus monkey.Recent Prog. Horm. Res., 30: 1–46.PubMedGoogle Scholar
  22. Koyama, T., A. de la Pena, &N. Hagino, 1977. Plasma estrogen, progestin, and luteinizing hormone during the normal menstrual cycle in the baboon: Role of luteinizing hormone.Amer. J. Obstet. Gynecol., 127: 67–71.Google Scholar
  23. Lawton, I. E., 1972. Facilitatory feedback effects of adrenal and ovarian hormones on LH secretion.Endocrinology, 90: 575–579.PubMedGoogle Scholar
  24. Lloyd, C. W., J. Lobotsky, D. Baird, J. McCracken, J. Weisz, M. Pupkin, J. Zanartu, &J. Puga, 1971. Concentrations of unconjugated estrogens, androgens, and gestagens in ovarian and peripheral venous plasma of women: the normal cycle.J. Clin. Endocrinol. Metab., 32: 155–166.PubMedGoogle Scholar
  25. MacKinnon, W. J., 1964. Table for both the sign test and distribution free confidence intervals of the median for sample sizes to 1000.J. Amer. Stat. Assoc., 59: 935–956.Google Scholar
  26. Maples, W. R., 1968. Classification of the Kenya baboon. Ph.D. Dissertation, Univ. of Texas, Austin, Texas.Google Scholar
  27. McNatty, K. P., A. Markis, C. Degrazia, R. Osathanondh, &K. J. Ryan, 1979. The production of progesterone, androgens and estrogens by granulosa cells, thecal tissue and stromal tissue from human ovaries in vitro.J. Clin. Endocrinol. Metab., 49: 687–699.PubMedGoogle Scholar
  28. Mikhail, G., 1970. Hormone secretion by the human ovaries.Gynecol. Invest., 1: 5–20.PubMedGoogle Scholar
  29. Moor, R. M., 1974. The ovarian follicle of the sheep: inhibition of estrogen secretion by luteinizing hormone.J. Endocrinol., 61: 455–463.PubMedGoogle Scholar
  30. Nequin, L. G. &N. B. Schwartz, 1971. Adrenal participation in the timing of mating and LH release in the cyclic rat.Endocrinology, 88: 325–331.PubMedGoogle Scholar
  31. Pauerstein, C. J., C. A. Eddy, H. D. Croxatto, R. Hess, T. M. Siler-Khoder, &H. B. Croxatto, 1978. Temporal relationships of estrogen, progesterone, and luteinizing hormone levels to ovulation in women and infrahuman primates.Amer. J. Obstet. Gynecol., 130: 876–886.Google Scholar
  32. Resko, J. A., M. J. Koering, R. W. Goy, &C. H. Phoenix, 1975. Preovulatory progestins: Observations on their source in the rhesus monkey.J. Clin. Endocrinol. Metab., 41: 120–125.PubMedGoogle Scholar
  33. Ross, G. T., C. M. Cargille, M. B. Lipsett, P. L. Rayford, J. R. Marshall, C. A. Strott, &D. Rodbard, 1970. Pituitary and gonadal hormones in women during spontaneous and induced ovulatory cycles.Recent Prog. Horm. Res., 26: 1–48.PubMedGoogle Scholar
  34. Saidapur, S. K. &G. S. Greenwald, 1978. Peripheral blood and ovarian levels of sex steroids in the cyclic hamster.Biol. Reprod., 18: 401–408.CrossRefPubMedGoogle Scholar
  35. Shaikh, A. A., 1972. Estrone, estradiol, progesterone and 17-α-hydroxyprogesterone in the ovarian venous plasma during the estrous cycle of the hamster.Endocrinology, 91: 1136–1140.PubMedGoogle Scholar
  36. ————,C. L. Celaya, I. Gomez &S. A. Shaikh, 1982. Temporal relationship of hormonal peaks to ovulation and sex skin deturgescence in the baboon.Primates, 23: 444–452.Google Scholar
  37. ————,M. J. K. Harper, 1972. Ovarian steroid secretion in estrous, mated, and HCG-treated rabbits determined by concurrent cannulation of both ovarian veins.Biol. Reprod., 7: 387–397.PubMedGoogle Scholar
  38. ————,S. A. Shaikh, 1975. Adrenal and ovarian steroid secretion in the rat estrous cycle temporally related to gonadotropins and steroid levels found in peripheral plasma.Endocrinology, 96: 37–44.PubMedGoogle Scholar
  39. ————, ————, &C. L. Celaya, 1981. Utero-ovarian vein blood collection by the use of a laparoscope in the baboon.Biol. Reprod., 24: 455–459.CrossRefPubMedGoogle Scholar
  40. ————, ————, ————, &J. W. Goldzieher, 1982. Ovulatory patterns in successive cycles and menstrual cycle characteristics in the baboon.Primates, 23: 592–595.Google Scholar
  41. Steel, R. G. D. &J. H. Torrie, 1980.Principles and Procedures of Statistics: A Biometric Approach (2nd ed.). McGraw-Hill Book Co., New York, pp. 137–194.Google Scholar
  42. Stevens, V. C., S. J. Sparks, &J. E. Powell, 1970. Level of estrogens, progesterones and luteinizing hormone during the menstrual cycle of the baboon.Endocrinology, 87: 658–666.PubMedGoogle Scholar
  43. Terranova, P. F., N. C. Martin, &S. Chien, 1982. Theca is the source of progesterone in experimentally induced atretic follicles of the hamster.Biol. Reprod., 26: 721.PubMedGoogle Scholar
  44. Tukey, J. W., 1977.Exploratory Data Analysis. Addison-Wesley Publ. Co., Reading, MA.Google Scholar
  45. Veldhuis, H. D., P. H. Klase, &J. M. Hammond, 1981. Direct actions of 17-β estradiol on progesterone production by highly differential porcine granulosa cells in vitro. II. Regulatory interaction of estradiol with luteinizing hormone and cycle nucleotides.Endocrinology, 109: 433–442.PubMedGoogle Scholar

Copyright information

© Japan Monkey Centre 1986

Authors and Affiliations

  • A. A. Shaikh
    • 1
  • E. E. Gbur
    • 2
  • S. A. Shaikh
    • 2
  1. 1.Department of Veterinary PhysiologyTexas A&M UniversityCollege StationUSA
  2. 2.The Institute of StatisticsTexas A&M UniversityCollege StationUSA

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