, Volume 34, Issue 1, pp 61–69 | Cite as

Highly repeated DNA analysis and systematics of the Lemuridae, a family of Malagasy prosimians

  • S. Crovella
  • D. Montagnon
  • Y. Rumpler


Hybridization of highly repeated DNA sequences ofEulemur fulvus mayottensis, Lemur catta, andVarecia has been performed on blots of different species of Lemuridae (L. catta, Hapalemur griseus, Varecia variegata variegata, V. v. rubra, E. macaco macaco, E. coronatus, E. mongoz, andE. rubriventer). The probe ofE. fulvus only hybridized with the differentEulemur species, whereas that ofVarecia hybridized with the two subspecies ofVarecia and that ofL. catta with bothL. catta andHapalemur. These results were used to confirm the classification ofVarecia in a separate genus and to review the separation of theL. catta/Hapalemur group from the other species ofEulemur. Comparison of the migration patterns from DNA fragments of these different species has been used to propose a cladogram of the differentEulemur species.

Key Words

Primates Lemuridae Varecia Lemur Hapalemur Eulemur Highly repeated DNA Taxonomy 


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  1. Affolter, M., 1938. Les organes cutanés d'Hapalemur griseus.Bull. Acad. Malgache, 8(20): 77–100.Google Scholar
  2. Andrew, R. J., 1964. The displays of the primates. In:Evolutionary and Genetic Biology of Primates, Vol. 2.J. Buettner-Janusch (ed.), Academic Press, New York, pp. 227–309.Google Scholar
  3. Andriamiandra, A., 1971. L'appareil génital femelle duLemur catta Linnaeus 1758: Histologie et histophysiologie.C. R. Ass. Anat., 151: 163–181.Google Scholar
  4. Dunaif, A. &I. Tattersall, 1991. Lemur species show differences in immunological and biological activity of luteinizing hormone.Folia Primatol., 57: 173–176.PubMedGoogle Scholar
  5. Felsenstein, J., 1987. PHYLIP (Phylogeny Inference Package), Ver. 3.0, Univ. of Washington.Google Scholar
  6. Gray, J. E., 1863. cited byHill, W. C. O., 1953.Primates: Comparative Anatomy and Taxonomy. III–IV: Lemuroidea. Edinburgh Univ. Press, Edinburgh.Google Scholar
  7. Groves, C. P., 1989.A Theory of Human and Primate Evolution. Clarendon Press, Oxford.Google Scholar
  8. ———— &R. H. Eaglen, 1988. Systematics of the Lemuridae (Primate, Strepsirhini).J. Human Evol., 17: 513–538.Google Scholar
  9. Hill, W. C. O., 1953.Primates: Comparative Anatomy and Taxonomy. I: Strepsirhini. Edinburgh Univ. Press, Edinburgh.Google Scholar
  10. Jones, K. W., 1978. Speculations on the functions of satellite DNA in evolution.Z. Morphol. Anthropol., 69: 113–171.Google Scholar
  11. Jung, K. Y., S. Crovella, &Y. Rumpler, 1992. Phylogenetic relationships among Lemuriform species determined from restriction genomic DNA banding patterns.Folia Primatol., 58: 224–229.PubMedGoogle Scholar
  12. Maniatis, T., E. F. Fritsch, &J. Sambrook, 1982.Molecular cloning. A laboratory manual (2nd edition)C. Nolan (ed.), Cold Spring Harbor Laboratory Press, New York.Google Scholar
  13. Montagna, W., 1962. The skin of lemurs.Ann. N. Y. Acad. Sc., 102: 190–209.Google Scholar
  14. Petter, J. J., 1962. Recherches sur l'écologie et l'éthologie des lémuriens malgaches.Mém. Mus. Natl Hist. Nat. Paris, 27: 1–146.Google Scholar
  15. ————,R. Albignac, &Y. Rumpler, 1977. Mammifères lémuriens (Primates, Prosimiens). In:Faune de Madagascar, n° 44. O.R.S.T.O.M.—C.N.R.S., Paris.Google Scholar
  16. Pocock, R. I., 1918. On the external characters of the lemurs and ofTarsius.Proc. Zool. Soc., 55: 19–53.Google Scholar
  17. Rumpler, Y., 1975. The significance of chromosomal studies in the systematics of the Malagasy lemurs. In:Lemur Biology,I. Tattersall &R. W. Sussmann (eds.), Plenum Press, New York, pp. 25–40.Google Scholar
  18. ————, &A. Andriaminadra, 1968. Sur l'étude des glandes brachiales et antébrachiales duLemur catta.C. R. Soc. Biol., 162: 1430–1433.Google Scholar
  19. ———— &B. Dutrillaux, 1978. Chromosomal evolution in Malagasy lemurs. III. Chromosome banding studies in the genusHapalemur and the speciesLemur catta.Cytogenet. Cell Genet., 21: 201–211.PubMedGoogle Scholar
  20. Schwartz, J. H. &I. Tattersall, 1985. Anthropology.Papers Amer. Mus. Nat. Hist. New York, 60(1): 1–100.Google Scholar
  21. Shedd, D. H. &J. M. Macedonia, 1991. Metachromism and its phylogenetic implications for the genusEulemur.Folia Primatol., 57: 221–231.Google Scholar
  22. Simons, E. &Y. Rumpler, 1988.Eulemur: new generic name for species ofLemur other thanLemur catta.C. R. Acad. Sci. Paris, 307 (Série III): 547–551.Google Scholar
  23. Southern, E. M., 1975. Detection of specific sequences among DNA fragments separated by gel exlectrophoresis.J. Mol. Biol., 38: 503–517.Google Scholar
  24. Tattersall, I., 1982.The primates of Madagascar. Columbia Univ. Press, New York.Google Scholar
  25. Tautz, D. &M. Renz, 1983. An optimized freeze-squeeze method for the recovery of DNA fragments from agarose gels.Analyt. Biochem., 132: 14–19.PubMedGoogle Scholar
  26. Templeton, A. R., 1983. Phylogenetic inference from restriction endonuclease cleavage site maps with particular reference to the humans and apes.Evolution, 37: 221–244.Google Scholar

Copyright information

© Japan Monkey Centre 1993

Authors and Affiliations

  • S. Crovella
    • 1
  • D. Montagnon
    • 1
  • Y. Rumpler
    • 1
  1. 1.Faculté de MedecineInstitut d'EmbryologieStrasbourg CedéxFrance

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