The blood vitamin analyses of the common marmoset (Callithrix jacchus) were determined to provide baseline reference values for the normal animal. Ascorbic acid, riboflavin (erythrocyte glutathione reductase) [ECR], measurement, thiamin (erythrocyte transketolase) measurement and vitamin A (retinol) were determined for Texas A&M colony-born animals and those obtained from the wild. The analyses were completed on the animals, three times each, for a total of 93 analyses, which included 51 colony-born and 60 wild-born marmosets. A mean value of 0.98 mg/dl for ascorbic acid was found for the colony with a range from 0.06 to 4.1 mg/dl. The normal range for the marmosets appeared to be 0.5 to 1.5 mg/dl. The mean activity coefficient (AC) for the marmosets was 1.0 indicating that the animals had adequate riboflavin in the diet. The mean transketolase activities were (ribose remaining −30.1 IU/L) and (sedoheptulose appearance −7.9 IU/L). The mean and range for serum vitamin A (retinol) were 20.4 mg/dl and 6.96–57.44 mg/dl, respectively. None of the animals (colony-born or wild-born) exhibited any clinical signs of vitamin deficiencies as a result of being maintained in an indoor-outdoor environment over a three-year period.
KeywordsAscorbic Acid Thiamin Glutathione Reductase Riboflavin Activity Coefficient
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- Anderson, E. T., J. P. Lewis, M. Passovoy &F. E. Trobaugh, Jr., 1967. Marmosets as laboratory animals. II. The hematology of laboratory kept marmosets.Lab. Anim. Care, 17: 30–40.Google Scholar
- Benjamin, M. M., 1961.Outline of Veterinary Clinical Pathology. The Iowa State Univ. Press, Ames, Iowa.Google Scholar
- Brin, M., 1964. Erythrocyte as a biopsy tissue for functional evaluation of thiamine adequacy.J. Amer. Med. Assoc., 187: 762–766.Google Scholar
- Dreyfus, P., 1962. Clinical application of blood transketolase determinations.New Engl. J. Med., 267: 596.Google Scholar
- DuPlessis, J. P., 1967. An evaluation of biochemical criteria for use in nutrition status surveys (National Nutrition Research Institute Bull. No. 11). In:Council for Scientific and Industrial Report No. 261, Pretoria, S. Africa, p. 126.Google Scholar
- Lehner, N. D., B. C. Bullock &T. B. Clarkson, 1968. Ascorbic acid deficiency in the squirrel monkey.Proc. Soc. Exp. Biol. Med., 128: 1442–1458.Google Scholar
- Levy, B. M., F. J. Stein, R. F. Sis &R. W. Lewis, 1978. Observations on a colony of cotton eared marmosetsCallithrix jacchus with some plans for future expansion.Prim. Med., 10: 63–70.Google Scholar
- McNees, D. W., 1981. Baseline blood chemistry determinations of wild versus colony born common marmosets (Callithrix jacchus) maintained in an indoor-outdoor environment. Ph.D. Thesis, Texas A&M Univ., College Station, Texas.Google Scholar
- ————,B. J. Caronia, R. W. Lewis &F. J. Stein, 1979. Effect of anticoagulant concentration in common marmoset (Callithrix jacchus) blood.Lab. Prim. Newsl., 18: 1–4.Google Scholar
- ———— &C. A. Kuether, 1943. The determination of ascorbic acid in whole blood and urine through the 2, 4, dinitrophenylhydrazine derivative of ascorbic acid.J. Biol. Chem., 147: 399–402.Google Scholar
- Suvarnaklich, K., G. V. Mann &F. J. Store, 1952. Riboflavin in human serum.J. Nutr., 47: 105.Google Scholar
- Center for Disease Control, 1972.Ten-state Nutrition Survey Reports. I–IV. Atlanta, Georgia.Google Scholar
- Warnock, L. G., 1957. A new approach to erythrocyte transketolase measurement.J. Nutr., 100: 1057–1062.Google Scholar