Ovum recovery and in vitro fertilization in crab-eating monkeys (Macaca fascicularis)
- 27 Downloads
A total of 301 oocytes were recovered from crab-eating monkeys and subjected to insemination in vitro resulting in two fertilized ova. Sixteen monkeys in 24 cycles received 37.5 IU of hMG daily from the second day of the menstrual cycle for 7 to 10 days. Oocytes were recovered under laparotomy at 20 to 49 hr after administration of 1,000–1,500 IU of hCG. The maturation rate of the recovered oocytes was 24.2% as judged from morphological criteria under the light microscope. With additional maturation culture, the rate increased to 36.2%. The matured oocytes were inseminated at 3 to 4 hr after aspiration using homologous spermatozoa which had been capacitated in vitro. Two oocytes were judged as being fertilized based on the presence of 3 and 5 pronuclei, respectively, when examined 12 hr after the insemination. This is the first report of in vitro fertilized ova in nonhuman primates in Japan.
Key WordsIn vitro fertilization (IVF) Crab-eating monkey (cynomolgus monkeyMacaca fascicularis) Electro-ejaculation of ovulation Polyspermy
Unable to display preview. Download preview PDF.
- Abramczuk, J., R. A. Stark, M. Konvinski, D. Solter, L. Mastroianni, &H. Koprowski, 1977. Parthenogenetic activation of rhesus monkey follicular oocytes in vitro.J. Embryol. Exp. Morph., 42: 115–126.Google Scholar
- Gould, K. G., 1979. Fertilization in vitro in nonhuman primate ova: Present status rationale for the further development of the technique. In:Report to the Ethics Advisory Board, Appendix: HEW Support of Research Involving Human in Vitro Fertilization and Embryo Transfer, U.S. Gov. Printing Off., No. 14, pp. 1–25.Google Scholar
- Honjo, S., F. Cho, &K. Terao, 1984. Establishing the cynomolgus monkey as a laboratory animal. In:Research on Non-human Primates. Advance in Veterinary Science and Comparative Medicine, 28,A. G. Hendrickx (ed.), Academic Press, New York, pp. 51–80.Google Scholar
- Jainudfen, M. R. &E. S. E. Hafez, 1973. Gonadotropin-induced multiple ovulation in the crabeating monkey,Macaca fascicularis.J. Reprod. Fertil., 33: 151–154.Google Scholar
- Kreitmann, O., A. Lynch, W. E. Nixon, &G. D. Hogen, 1982. Ovum collection, induced luteal dysfunction, in vitro fertilization, embryo development and low tubal ovum transfer in primates. In:In Vitro Fertilization and Embryo Transfer,E. S. E. Hafez &K. Semm (eds.), MTP, Lancaster, England, pp. 303–324.Google Scholar
- van Wagenen, G. W. &M. E. Simpson, 1973.Postnatal Development of the Ovary in Homo sapiensand Macaca mulattaand Induction of Ovulation in the Macaque. Yale Univ. Press, New Haven, Connecticut.Google Scholar
- Yoshida, T., M. Nakama, A. Hiyaoka, M. Suzuki, F. Cho, &S. Honjo, 1982. Menstrual cycle lengths and the estimated time of ovulation in the cynomolgus monkey (Macaca fascicularis).Exp. Anim., 31: 165–174.Google Scholar
- ,K. Yokota, F. Cho, &S. Honjo, 1984. Circulating levels of gonadotropins and ovarian luteinizing hormone-receptor in female cynomolgus monkeys. In:Gunma Symposia on Endocrinology, 21, Center for Academic Publications, Japan, Tokyo, pp. 107–125.Google Scholar