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Primates

, Volume 29, Issue 1, pp 107–116 | Cite as

Ovum recovery and in vitro fertilization in crab-eating monkeys (Macaca fascicularis)

  • Kenji Ida
  • Yoichi Noda
  • Juri Yano
  • Aisaku Fukuda
  • Hisashi Matsumoto
  • Takahide Mori
Article

Abstract

A total of 301 oocytes were recovered from crab-eating monkeys and subjected to insemination in vitro resulting in two fertilized ova. Sixteen monkeys in 24 cycles received 37.5 IU of hMG daily from the second day of the menstrual cycle for 7 to 10 days. Oocytes were recovered under laparotomy at 20 to 49 hr after administration of 1,000–1,500 IU of hCG. The maturation rate of the recovered oocytes was 24.2% as judged from morphological criteria under the light microscope. With additional maturation culture, the rate increased to 36.2%. The matured oocytes were inseminated at 3 to 4 hr after aspiration using homologous spermatozoa which had been capacitated in vitro. Two oocytes were judged as being fertilized based on the presence of 3 and 5 pronuclei, respectively, when examined 12 hr after the insemination. This is the first report of in vitro fertilized ova in nonhuman primates in Japan.

Key Words

In vitro fertilization (IVF) Crab-eating monkey (cynomolgus monkeyMacaca fascicularisElectro-ejaculation of ovulation Polyspermy 

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Copyright information

© Japan Monkey Centre 1988

Authors and Affiliations

  • Kenji Ida
    • 1
    • 2
  • Yoichi Noda
    • 1
  • Juri Yano
    • 1
  • Aisaku Fukuda
    • 1
  • Hisashi Matsumoto
    • 1
  • Takahide Mori
    • 1
  1. 1.Department of Gynecology and Obstetrics, Faculty of MedicineKyoto UniversityKyotoJapan
  2. 2.Kyoto Senbai HospitalKyotoJapan

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