Abstract
A total of 301 oocytes were recovered from crab-eating monkeys and subjected to insemination in vitro resulting in two fertilized ova. Sixteen monkeys in 24 cycles received 37.5 IU of hMG daily from the second day of the menstrual cycle for 7 to 10 days. Oocytes were recovered under laparotomy at 20 to 49 hr after administration of 1,000–1,500 IU of hCG. The maturation rate of the recovered oocytes was 24.2% as judged from morphological criteria under the light microscope. With additional maturation culture, the rate increased to 36.2%. The matured oocytes were inseminated at 3 to 4 hr after aspiration using homologous spermatozoa which had been capacitated in vitro. Two oocytes were judged as being fertilized based on the presence of 3 and 5 pronuclei, respectively, when examined 12 hr after the insemination. This is the first report of in vitro fertilized ova in nonhuman primates in Japan.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abramczuk, J., R. A. Stark, M. Konvinski, D. Solter, L. Mastroianni, &H. Koprowski, 1977. Parthenogenetic activation of rhesus monkey follicular oocytes in vitro.J. Embryol. Exp. Morph., 42: 115–126.
Balmaceda, J. P., T. B. Pool, J. B. Arana, T. S. Heitman, &R. H. Ash, 1984. Successful in vitro fertilization and embryo transfer in cynomolgus monkeys.Fertil. Steril., 42: 791–795.
Bavister, B. D., D. E. Boatman, K. Collins, D. J. Dierschke, &S. G. Eisele, 1984. Birth of rhesus monkey infant after in vitro fertilization and nonsurgical embryo transfer.Proc. Natl. Acad. Sci., 81: 2218–2222.
,L. Leibfried, M. W. Vernon, 1983. Fertilization and cleavage of rhesus monkey oocytes in vitro.Biol. Reprod., 28: 983–999.
Boatman, D. E. &B. D. Bavister, 1984. Stimulation of rhesus monkey sperm capacitation by cyclic nucleotide mediators.J. Reprod. Fertil., 71: 357–366.
Cho, F., 1981. Breeding performance of the cynomolgus monkey at Tsukuba Primate Center for Medical Science.Jap. J. Med. Sci. Biol., 34: 252–255.
Clayton, O. &T. J. Kuehl, 1984. The first in vitro fertilization and embryo transfer in a nonhuman primate.Theriogenology, 21: 228–232.
Czaja, J. A., J. A. Robinson, S. G. Eisele, G. Scheffler, &R. W. Goy, 1977. Relationship between sexual skin colour of female rhesus monkeys and midcycle plasma levels of oestradiol and progesterone.J. Reprod. Fertil., 49: 147–150.
Dierschke, D. J. &J. R. Clark, 1976. Laparoscopy inMacaca mulatta: Specialized equipment employed and initial observations.J. Med. Primatol., 5: 100–110.
Dukelow, W. R., 1970. Induction and timing of single and multiple ovulations in the squirrel monkey (Saimiri sciureus).J. Reprod. Fertil., 22: 303–309.
Gould, K. G., 1979. Fertilization in vitro in nonhuman primate ova: Present status rationale for the further development of the technique. In:Report to the Ethics Advisory Board, Appendix: HEW Support of Research Involving Human in Vitro Fertilization and Embryo Transfer, U.S. Gov. Printing Off., No. 14, pp. 1–25.
, 1983. Ovum recovery and in vitro fertilization in the chimpanzee.Fertil. Steril., 40: 378–383.
,E. M. Cline, &E. M. Williams, 1973 Observations on the induction of ovulation and fertilization in vitro in the squirrel monkey (Saimiri sciureus).Fertil. Steril., 24: 260–268.
Honjo, S., F. Cho, &K. Terao, 1984. Establishing the cynomolgus monkey as a laboratory animal. In:Research on Non-human Primates. Advance in Veterinary Science and Comparative Medicine, 28,A. G. Hendrickx (ed.), Academic Press, New York, pp. 51–80.
Jainudfen, M. R. &E. S. E. Hafez, 1973. Gonadotropin-induced multiple ovulation in the crabeating monkey,Macaca fascicularis.J. Reprod. Fertil., 33: 151–154.
Jewett, D. A. &W. R. Dukelow, 1973. Follicular observation and laparoscopic aspiration techniques inMacaca fascicularis.J. Med. Primatol., 2: 108–115.
Kreitmann, O., A. Lynch, W. E. Nixon, &G. D. Hogen, 1982. Ovum collection, induced luteal dysfunction, in vitro fertilization, embryo development and low tubal ovum transfer in primates. In:In Vitro Fertilization and Embryo Transfer,E. S. E. Hafez &K. Semm (eds.), MTP, Lancaster, England, pp. 303–324.
Steptoe, P. C. &R. G. Edwards, 1978. Birth after reimplantation of human embryo.Lancet, 2: 366.
van Wagenen, G. W. &M. E. Simpson, 1973.Postnatal Development of the Ovary in Homo sapiensand Macaca mulattaand Induction of Ovulation in the Macaque. Yale Univ. Press, New Haven, Connecticut.
Wan, L. &H. Balin, 1969. Induction of ovulation in rhesus monkeys: A comparative study.Fertil. Steril., 20: 111–126.
Yoshida, T., M. Nakama, A. Hiyaoka, M. Suzuki, F. Cho, &S. Honjo, 1982. Menstrual cycle lengths and the estimated time of ovulation in the cynomolgus monkey (Macaca fascicularis).Exp. Anim., 31: 165–174.
,K. Yokota, F. Cho, &S. Honjo, 1984. Circulating levels of gonadotropins and ovarian luteinizing hormone-receptor in female cynomolgus monkeys. In:Gunma Symposia on Endocrinology, 21, Center for Academic Publications, Japan, Tokyo, pp. 107–125.
Author information
Authors and Affiliations
About this article
Cite this article
Ida, K., Noda, Y., Yano, J. et al. Ovum recovery and in vitro fertilization in crab-eating monkeys (Macaca fascicularis). Primates 29, 107–116 (1988). https://doi.org/10.1007/BF02380853
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02380853