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Cyclic AMP stimulates45Ca-accumulation via synthesis of insulin-like growth factor-I in cultures of osteoblast-like cells

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Summary

The effects of cAMP on insulin-like growth factor-I (IGF-I) secretion and45Ca-accumulation into extracellular matrix were investigated in cloned osteoblast-like MC3T3-E1 cells cultured for 7 weeks, which was considered to be long enough to develop calcification. Dibutyryl cAMP stimulated IGF-I secretion dose dependently in the range between 0.1 mM and 3 mM. Dibutyryl cAMP also stimulated accumulation of45Ca into sodium dodecyl sulfate-insoluble, EDTA-extractable materials in cultures of these cells dose dependently between 0.1 mM and 3 mM. The patterns of both stimulations were similar. The dibutyryl cAMP-stimulated IGF-I secretion preceded the45Ca-accumulation. Antibodies to IGF-I suppressed the dibutyryl cAMP-stimulated45Ca-accumulation almost to the control level. Taking account of our previous report that exogenous IGF-I stimulates45Ca-accumulation in cultures of these cells, the present results suggest that cAMP stimulates45Ca-accumulation via synthesis of IGF-I in osteoblast-like cells.

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Authors and Affiliations

  1. First Department of Internal Medicine, Nagoya University School of Medicine, 466, Nagoya, Japan

    Jun Kotoyori, Osamu Kozawa, Haruhiko Tokuda, Masaichi Miwa & Yutaka Oiso

  2. Department of Biochemistry, Institute for Developmental Research, Aichi Prefectural Colony, 480-03, Aichi, Japan

    Osamu Kozawa

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  1. Jun Kotoyori
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  2. Osamu Kozawa
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  3. Haruhiko Tokuda
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  4. Masaichi Miwa
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  5. Yutaka Oiso
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Kotoyori, J., Kozawa, O., Tokuda, H. et al. Cyclic AMP stimulates45Ca-accumulation via synthesis of insulin-like growth factor-I in cultures of osteoblast-like cells. J Bone Miner Metab 10, 1–5 (1992). https://doi.org/10.1007/BF02378976

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  • DOI: https://doi.org/10.1007/BF02378976

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