Plant and Soil

, Volume 105, Issue 2, pp 241–246 | Cite as

Associative effect of Rhizobium and phosphate-solubilizing bacteria on the yield and nutrient uptake of chickpea

  • A. R. Alagawadi
  • A. C. Gaur


Combined inoculation of Rhizobium and ‘Phosphate-solubilizing’Pseudomonas striata orBacillus polymyxa with and without added chemical fertilizer on chickpea yield and nutrient content was studied under greenhouse conditions. While the single inoculation of Rhizobium increased the nodulation and nitrogenase activity, the ‘phosphate-solubilizers’ increased the available phosphorus content of the soil. Combined inoculation of Rhizobium andP. striata orB. polymyxa increased the above parameters and also the dry matter content, the grain yield and nitrogen and phosphorus uptake significantly over the uninoculated control. The inoculation effects were more pronounced in the presence of added fertilizers. The possibilities of saving half the dose of N and replacing superphosphate with rockphosphate and inoculation with ‘phosphate-solubilizers’ are discussed.

Key words

Bacillus polymyxa Cicer arietinum nutrient uptake plant yield Pseudomonas striata Rhizobium 


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  1. Barea, J M, Navarro E and Montoya E 1976 Production of plant growth regulators by rhizosphere phosphate-solubilizing bacteria. J. Appl. Bact. 40, 128–134.Google Scholar
  2. Brown M E 1974 Seed and root bacterization. Annu. Rev. Phytopathol. 12, 181–198.CrossRefGoogle Scholar
  3. Cochran W G and Cox G M 1957 Experimental Designs. Second Edition, John Wiley and Sons Inc. New York.Google Scholar
  4. Dorosinsky L M and Kadyrov A A 1975 Effect of inoculation on nitrogen fixation by chickpea, its crop and content of protein. Mikrobiologiya 44, 1103–1106.Google Scholar
  5. Gaur A C 1972 Role of phosphate-solubilizing microorganisms and organic matter in soil productivity. Symp. Soil Productivity: Nat. Acad. Sci. India, pp 259–268.Google Scholar
  6. Gaur A C 1985 Phosphate-solubilizing microorganisms and their role in plant growth and crop yields. Proc. Soil Biol. Symp., Hissa-r, India, pp 125–138.Google Scholar
  7. Gaur A C, Arora D and Prakash N 1979 Electron microscopy of some rockphosphate dissolving bacteria and fungi. Folio Micriobiol. 24, 314–317.Google Scholar
  8. Gaur A C, Ostwal K P and Mathur R S 1980 Save superphosphate by using phosphate-solubilizing cultures and rock phosphate. Kheti 32, 23–25.Google Scholar
  9. Hernandez L G and Hill G S 1983 Effect of plant population and inoculation on yield and yield components of chickpea (Cicer arietinum). Proc. Agron. Soc. NZ. 13, 75–79.Google Scholar
  10. Jackson M L 1958 Soil Chemical Analysis, Prentice Hall Inc. Englewood Cliffe NJ. pp 151–153 and 331–334.Google Scholar
  11. Kundu B S and Gaur A C 1980 Establishment of nitrogen fixing and phosphate solubilizing bacteria in rhizosphere and their effect on yield and nutrient uptake of wheat crop. Plant and Soil 57, 223–230.CrossRefGoogle Scholar
  12. Lu K C, Gilmour C M, Zagallo A C and Bollen W B 1958 Effect of gibberelic acid on soil microorganisms. Nature 18, 189–190.Google Scholar
  13. Olson S R, Cole C V, Watanabe F S and Dean L A 1954 Estimation of available phosphorus in soil by extraction with sodium bicarbonate. USDA circular No. 939.Google Scholar
  14. Pareek R P and Gaur A C 1973 Release of phosphate from tricalcium and rock phosphate by organic acids. Curr. Sci. 42, 278–279.Google Scholar
  15. Patil R N and Shinde P A 1980 Studies on nodulation pattern in gram (Cicer arietinum L.). J. Maharashtra Agric. Univ. 5, 211–213.Google Scholar
  16. Pikovskaya R I 1948 Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Mikrobiologiya 17, 362–370.Google Scholar
  17. Sattar M A and Gaur A C 1987 Production of auxins and gibberellins by phosphate dissolving microorganisms. Zbl. Mikrobiol. (in press).Google Scholar
  18. Sullia S B 1968 Effect of foliar sprays of hormones on the rhizosphere microflora of leguminous seeds. Plant and Soil 29, 292–298.CrossRefGoogle Scholar
  19. Technicon Monograph I 1971 Technicon Private Limited, Tarrytonis, New York.Google Scholar
  20. Vincent J M 1970 A Manual for the Practical Study of Root Nodule Bacteria. IBP handbook No. 15, Blackwell Scientific Pub. Oxford.Google Scholar

Copyright information

© Martinus Nijhoff Publishers 1988

Authors and Affiliations

  • A. R. Alagawadi
    • 1
  • A. C. Gaur
    • 1
  1. 1.Division of MicrobiologyIndian Agricultural Research InstituteNew DelhiIndia

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