Advertisement

Plant and Soil

, Volume 96, Issue 3, pp 347–358 | Cite as

Influence de pH acides sur la viabilité d'isolats deFrankia

  • M. Faure-Raynaud
  • M. A. Bonnefoy-Poirier
  • A. Moiroud
Article

Résumé

Vingt souches deFrankia, isolées à partir de 7 espèces différentes de plantes actinorhiziennes ont été cultivées sur des milieux maintenus à des pH constants de 5; 4,8; 4,6 et 4,2. Chaque souche a été cultivée 2 à 3 fois consécutives à un même pH. Quatorze souches ne montrent aucune croissance à pH 5. Seules 3 souches résistent à un séjour de plusieurs semaines à pH 4,6. Aucune souche ne demeure viable après un séjour dans un milieu à pH 4,2. Les 3 souches acido-résistantes supportent également la présence d'Al+++ libre dans le milieu de culture. Une forte concentration d'H+ dans le milieu stimule beaucoup la sporogénèse.

Inoculée dans un sol acide une souche acido-résistante conserve une chance de survie nettement supérieure à celle d'une souche neutrophile maintenue dans les mêmes conditions.

Mots clés

Alnus Frankia Nodules pH acides Plantes à actinorhizes Viabilité 

Low pH influence onFrankia strains viability

Summary

TwentyFrankia strains isolated from seven actinorhizal species were cultivated on media at various pH 5; 4.8; 4.6 and 4.2. Each strain was cultivated 2 or 3 times on the same pH. Fourteen strains did not grow at pH 4.6. At pH 4.2 all the strains died after few days. The 3 acido-resistant strains grew with free Al+++ in the culture medium.

Inoculated in an acidic soil, the viability of an acido-resistant strain was greater than that of a neutrophilic strain.

Key words

Actinorhizal plant Alnus Frankia Low pH Nodules Viability 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Références

  1. 1.
    Becking J H 1977 Nitrogen fixation in higher plants others than legumes.In Dinitrogen Fixation II. Eds. R W F Hardy and W S Silver, pp. 185–275. Wiley and Sons Inc. Publi. New York.Google Scholar
  2. 2.
    Bermudez de Castro F, Miguel C and Rodrigueż-Barrueco C 1976 A Study of the capacity of soils to induce nodules inAlnus glutinosa (L.) Gaertn andMyrica gale (L.) with spectral reference to the specificity of the endophytes. Ann. Microbiol. Inst. Pasteur 127A, 307–315.Google Scholar
  3. 3.
    Bitton G and Koopman B 1982 Tetrazolium reduction malachite green method for assessing the viability of filamentous bacteria in activated sludge. Appl. Environ. Microbiol. 43, 964–966.PubMedGoogle Scholar
  4. 4.
    Bond G 1976 The results of the IBP survey of root-nodule formation in non-leguminous angiosperms.In Symbiotic Nitrogen Fixation in Plants. Ed. P S Nutman, International Biological Program 7, Cambridge, pp 443–474. Cambridge University Press.Google Scholar
  5. 5.
    Bond G 1983 Taxonomy and distribution of non-legume nitrogen-fixing system.In Biological Nitrogen Fixation in Forest Ecosystems: Foundations and Applications. Eds. J C Gordon and C T Wheeler, pp 55–87.Google Scholar
  6. 6.
    Bonneau M et Souchier B 1979 Pédologie. Constituants et propriétés des sols. Masson Edit., 459 p.Google Scholar
  7. 7.
    Burggraaf A J P and Shipton W A 1982 Estimates ofFrankia growth under various pH and temperature regimes. Plant and Soil 61, 135–147.Google Scholar
  8. 8.
    Crocker L and Major J 1955 Soil development in relation to vegetation and surface age at Glacier Bay, Alaska. J. Ecol. 43, 427–448.Google Scholar
  9. 9.
    Danière C, Capellano A et Moiroud A 1986 Dynamique de l'azote dans un peuplement d'Alnus incana (L.) Moench: exemple de l'aulnaie du col d'Ornon, Isère. Acta Oecologica, 7 (2) 165–175.Google Scholar
  10. 10.
    Dijk C van 1979 Endophyte distribution in the soil.In Symbiotic Nitrogen Fixation in the Management of Temperate Forests. Eds J C Gordon, C T Wheeler and D A Perry, pp 84–94. Forest Research Lab., Oregon State Univ. Corvallis.Google Scholar
  11. 11.
    Dixon R O and Wheeler C T 1983 Biochemical, physiological and environmental aspects of symbiotic nitrogen fixation.In Biological Nitrogen Fixation in Forest Ecosystems: Foundations and Applications. Eds. J C Gordon and C T Wheeler, pp 107–181.Google Scholar
  12. 12.
    Duchaufour P 1970 Précis de Pédologie, Masson Edit. Paris, 475 p.Google Scholar
  13. 13.
    Faure-Raynaud M, Bonnefoy M A, Perradin Y Simonet P and Moiroud A 1984 Protoplast formation fromFrankia strains. Microbios 41, 159–166.Google Scholar
  14. 14.
    Griffiths A P and McCormick L H 1984 Effects of soil acidity on nodulation ofAlnus glutinosa and viability ofFrankia. Plant and Soil 79, 429–434.CrossRefGoogle Scholar
  15. 15.
    Hensley D L and Carpenter P L 1984 Effect of lime additions to acid stripmine spoil on survival, growth and nitrogen fixation (acetylene reduction) of several woody legume and actinomycete-nodulated species. Plant and Soil 79, 353–367.CrossRefGoogle Scholar
  16. 16.
    Houwers A and Akkermans A D L 1981 Influence of inoculation on yield ofAlnus glutinosa in the Netherlands. Plant and Soil 61, 189–202.CrossRefGoogle Scholar
  17. 17.
    Mayz de Manzi J and Cartwright P M 1984 The effects of pH and aluminium toxicity on the growth and symbiotic development of cowpeas (Vigna unguiculata (L.) Walp). Plant and Soil 80, 423–430.Google Scholar
  18. 18.
    Murry M A, Fontaine M S and Torrey J G 1984 Growth kinetics and nitrogenase induction inFrankia sp. HFP ArI3 grown in batch culture. Plant and Soil 78 61–78.CrossRefGoogle Scholar
  19. 19.
    Padan E 1984 Adaptation of bacteria to external pH.In Current Perspective in Microbial Ecology. Eds. M J Klug and C A Reddy. pp 49–55. American Society for Microbiology, Washington DC pp 49–55.Google Scholar
  20. 20.
    Pizelle G 1972 Les Angiospermes non-légumineuses fixatrices symbiotiques d'azote présentes dans la flore francaise. Bull. ENSAIA, Nancy XIV, 177–191.Google Scholar
  21. 21.
    Richard L 1967 Aire et répartition de l'aulne vert. Doc. Carte Veg. Alpes V, 81–113.Google Scholar
  22. 22.
    Silvester W 1977 Dinitrogen fixation by plant associations excluding legumes.In A Treastise on Dinitrogen Fixation. Section IV. Agronomy and Botany. Eds. R W F Hardy and A H Gibson. Wiley J and Sons Inc. New York. pp 141–190.Google Scholar
  23. 23.
    Stewart W D P, Fitzgerald G P and Burris R H 1967In situ studies on N2 fixation using the acetylene reduction technique. Proc. Natl. Acad. Sci. USA 58, 2071–2078.PubMedGoogle Scholar
  24. 24.
    Wheeler C T, McLaughlin M E and Steele P 1981 A comparison of symbiotic nitrogen fixation in Scotland inAlnus glutinosa andAlnus rubra. Plant and Soil 61, 169–188.CrossRefGoogle Scholar
  25. 25.
    Wood M, Cooper J E and Holding A J 1984 Soil acidity factors and nodulation ofTrifolium repens. Plant and Soil 78, 367–379.Google Scholar
  26. 26.
    Wood M, Cooper J E and Holding A J 1984 Aluminium toxicity and nodulation ofTrifolium repens. Plant and Soil 78, 381–391.Google Scholar

Copyright information

© Martinus Nijhoff Publishers 1986

Authors and Affiliations

  • M. Faure-Raynaud
    • 1
  • M. A. Bonnefoy-Poirier
    • 1
  • A. Moiroud
    • 1
  1. 1.Ecologie MicrobienneUniversité Claude Bernard, Lyon I Département de Biologie VégétaleVilleurbanne CedexFrance

Personalised recommendations