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Soil temperature effects on competitiveness and growth ofRhizobium japonicum and on Rhizobium-induced chlorosis of soybeans

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Summary

The effects of temperature on growth in broth and soil and on competition for nodule formation betweenRhizobium japonicum serotypes USDA 76 and 94 compared to 6 and 110 were studied. Increasing root temperatures of Lee soybean from 20 to 35°C increased the competitiveness of 76 and 94 relative to 6 and 110 for all inoculum ratios such that at 30 and 35°C symptoms ofRhizobium-induced chlorosis appeared. Tolerance to elevated temperatures was exhibited by 76 and 110, but not 94 and 6 in broth and soil which suggested that increased competitiveness of 76 and 94 at high soil temperatures was not dependent upon growth at elevated temperatures. Nodulation and vegetative growth of Lee soybeans were at a minimum at 20°C and optimum at 30°C. Differences in competitiveness of 6 to previous studies indicated the need to standardize temperatures of assays. Differences in growth responses of 76 and 94 to temperature from a previous study suggested a confounding effect on different carbon sources in growth media.

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Authors and Affiliations

  1. Department of Agronomy and Soils, University of Puerto Rico, Mayaquez, Puerto Rico

    R. A. Kluson

  2. Department of Agronomy, University of Maryland, 20742, College Park, MD, USA

    W. J. Kenworthy

  3. Nitrogen Fixation and Soybean Genetics Laboratory, USDA, 20705, Beltsville, MD, USA

    D. F. Weber

Authors
  1. R. A. Kluson
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  2. W. J. Kenworthy
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  3. D. F. Weber
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Additional information

Scientific Article No. A-3721 Contribution No. 6697 of the Maryland Agric Exp Sta, Dept of Agronomy, College Park, MD 20742 and the USDA, ARS, Beltsville, MD 20705. Part of a thesis submitted by the senior author in partial fulfillment of the requirements for the M.S. Degree.

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Kluson, R.A., Kenworthy, W.J. & Weber, D.F. Soil temperature effects on competitiveness and growth ofRhizobium japonicum and on Rhizobium-induced chlorosis of soybeans. Plant Soil 95, 201–207 (1986). https://doi.org/10.1007/BF02375072

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  • DOI: https://doi.org/10.1007/BF02375072

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