Plant and Soil

, Volume 96, Issue 1, pp 69–76 | Cite as

Interactions of exposure time and temperature on thermostability and protein content of excisedIllicium parviflorum roots

  • Dewayne L. Ingram
  • Patricia G. Webb
  • R. Hilton Biggs
Article

Summary

A model was developed to describe interactive effects of exposure time and treatment on thermostability of excisedIllicium parviflorum Michx. root cell membranes using electrolyte leakage (Lc) procedures. Roots were moved from 25°C to treatment temperatures between 35°C and 60°C for 30 to 300 min. A sigmoidal response described Lc increases with increasing temperature at selected time exposures and the lethal exposure time decreased exponentially as temperature increased. The lethal temperature (52.0±1.1°C) for a 15 min exposure using this technique was comparable to the critical temperature (52.2±1.2°C) when roots were exposed to gradually increasing temperatures (4°C per h). Total protein content of roots began to decrease as temperatures increased from 35 to 40°C and the temperature corresponding to 50% reduction in total proteins was 49.1±2.2°C.

Key words

Electrolyte leakage Heat stress Membrane thermostability Protein content 

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Literature cited

  1. 1.
    Alexandrov V Y and Lomagin A G 1970 The responsive increase in thermostability of plant cells. Protoplasma 69, 417–458.CrossRefGoogle Scholar
  2. 2.
    Altshuler M and Mascarenhas J P 1982 Heat shock proteins and effects of heat shock in plants. Plant Mol. Biol. 1, 103–115.Google Scholar
  3. 3.
    Becwar, M R, Wallner S J and Butler J D 1983 Effect of water stress onin vitro heat tolerance of turfgrass leaves. HortScience 18, 93–95.Google Scholar
  4. 4.
    Chen H H, Shen Z Y and Li P H 1982 Adaptability of crop plants to high temperature stress. Crop Sci. 22, 719–725.Google Scholar
  5. 5.
    Cooper P and Ho T H D 1983 Heat shock proteins in maize. Plant Physiol. 71, 215–222.Google Scholar
  6. 6.
    Fretz T A 1971 Influence of physical conditions on summer temperatures in nursery containers. HortScience 6, 400–401.Google Scholar
  7. 7.
    Furmanski R J and Buesher R W 1979 Influence of chilling on electrolyte leakage and internal conductivity of peach fruits. HortScience 14, 167–168.Google Scholar
  8. 8.
    Gur A, Mizirahi Y and Samish R M 1976 The influence of root temperature on apple trees. II. Clonal differences in susceptibility to damage caused by supraoptimal root temperature. J. Hort. Sci. 51, 195–202.Google Scholar
  9. 9.
    Ingram D L 1981 Characterization of temperature fluctuations and woody plant growth in white poly bags and conventional black containers. HortScience 16, 762–763.Google Scholar
  10. 10.
    Ingram D L 1985 Modeling high temperature and exposure time interactions onPittosporum tobira Thunb. root cell membrane thermostability. J. Am. Soc. Hort. Sci. 110, 470–473.Google Scholar
  11. 11.
    Ingram D L 1985 Heat tolerance of root cell membranes of two holly species. J. Am. Soc. Hort. Sci. 111 (2), (in press).Google Scholar
  12. 12.
    Ingram D L and Buchanan D 1981 Measurement of direct heat injury of roots of three woody plants. HortScience 16, 769–771.Google Scholar
  13. 13.
    Ingram D L and Buchanan D W 1984 Lethal high temperatures for roots of three citrus rootstocks. J. Am. Soc. Hort. Sci. 109, 189–193.Google Scholar
  14. 14.
    Ingram K T, Herzog D C, Boote K J, Jones J W and Barfield C S 1981 Effects of defoliating pests on soybean canopy CO2 exchange and reproductive growth. Crop Sci. 21, 961–968.Google Scholar
  15. 15.
    Key J L, Lin C Y and Chen Y M 1981 Heat shock proteins of higher plants. Proc. Natl. Acad. Sci. USA 78, 3526–3531.Google Scholar
  16. 16.
    Levitt J 1980 Response of plants to environmental stresses. Vol. I. Chilling, freezing and high temperature stresses. Academic press, New York.Google Scholar
  17. 17.
    McAlister L and Finkelstein D B 1980 Heat shock proteins and thermal resistance in yeast. Biochem. Biophys. Res. Commun. 93, 819–824.CrossRefPubMedGoogle Scholar
  18. 18.
    Minton K W, Karwin G M and Minton A P 1982 Nonspecific stabilization of stress-susceptible proteins by stress-resistant proteins: A model for biological role of heat shock proteins. Proc. Natl. Acad. Sci. USA 79, 7107–7111.PubMedGoogle Scholar
  19. 19.
    Sullivan C Y 1972 Mechanisms of heat and drought resistance in grain sorghum and methods of measurement. pp. 247–264.In Sorghum in the Seventies. Eds. N G P Rao and L R House. Oxford and I.B.H. Publishing Co. New Delhi, India.Google Scholar
  20. 20.
    Wu M T and Wallner S J 1984 Heat stress responses in cultured plant cells. Plant Physiol. 75, 778–780.Google Scholar
  21. 21.
    Young K and Hammett D R Q 1980 Temperature patterns in exposed black polyethylene plant containers. Agr. Mererol. 21, 165–172.Google Scholar

Copyright information

© Martinus Nijhoff Publishers 1986

Authors and Affiliations

  • Dewayne L. Ingram
    • 1
  • Patricia G. Webb
    • 1
  • R. Hilton Biggs
    • 1
  1. 1.Departments of Ornamental Horticulture and Fruit Crops, IFASUniversity of FloridaGainesvilleUSA

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