Skip to main content

Advertisement

SpringerLink
Log in

Vitamin D and calcium metabolism in hypertension and vascular smooth muscle cell function

  • Original Article
  • Published:
Journal of Bone and Mineral Metabolism Aims and scope Submit manuscript

Abstract

Disturbances in extracellular (systemic) and intracellular calcium metabolism have been demonstrated. Intracellular free calcium in vascular smooth muscle cells is elevated, which is believed to be the final determinant of vascular tone, thus blood pressure. This hypothesis is supported by the fact that calcium channel blocker reduces blood pressure in hypertension by blocking both receptor-operated and potential-operated calcium channel. On the other hand, calcium balance appears to be negative in hypertension and dietary supplement of calcium has been demonstrated to be effective in reduction of blood pressure in both animals and humans with high blood pressure. The latter observation seems to be opposite to the effect of calcium channel blocker, since restored calcium balance may increase intracellular free calcium concentration by supplying higher amount of extracellular (plasma) calcium to the cell membmane, which is leaky in hypertension. The effect of dietary calcium supplement may be explained by changes in calcium regulating hormones but not calciumper se. Recent data on calcium metabolism in the spontaneously hypertensive rats and effect of vitamin D in vascular smooth muncle cells will be discussed in relation to the regulation of vascular tone and blood pressure.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Partridge, N.C., Alcorn, D., Michelangeli, V.P., Ryan, G. and Martin, T.J.: Morphological and biochemical characterization of four clonal osteogenic sarcoma cell lines of rat origin. Cancer Res. 43:4308–4314,1983.

    CAS  PubMed  Google Scholar 

  2. Aubin, J.E., Heerche, J.N.M., Merrilees, M.J. and Sodek, J.: Isolation of bone cell clones with differences in growth, hormone responses, and extracellular matrix production. J.Cell Biol. 92:452–461,1982.

    Article  CAS  PubMed  Google Scholar 

  3. Fraser, J.D., Otawara, Y. and price, P.A.: 1,25-dihydroxyvitamin D3 stimulates the synthesis of matrix γ-carboxyglutamic acid protein by osteosarcoma cells. J.Biol.Chem. 263:911–916,1988.

    CAS  PubMed  Google Scholar 

  4. Guenther, H.L., Hofstetter, W., Stutzer, A., Mühlbauer, R. and Fleish, H.: Evidence for heterogeneity of the osteoblastic phenotype determined with clonalrat bone cells established from transforming growth factor-β induced cell colonies grown anchorage independently in semisolid medium. Endocrinology 125:2092–2102,1989.

    CAS  PubMed  Google Scholar 

  5. Muto, T.: Experimental production of osteosarcomas by using32P and 4NQO in inbred F 344 rats and their transplantability. Jpn. J. Oral Maxillofac. Surg. 34:2119–2138,1988

    Google Scholar 

  6. Takahashi, K., Akiyama, Y., Kanazawa, H., Sato, K.: Establishment and biological studies of rat osteosarcoma cell line (MSK). J.Jpn.Stomatol.Soc. 38:870–888,1989.

    Google Scholar 

  7. Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J.: Protein measurement with the folin phenol reagent. J. Biol.Chem. 193:265–275,1951.

    CAS  PubMed  Google Scholar 

  8. Inayama, S., Shibata, T., Ohtsuki, J. and Saito, S.: A new microanalytical method for determination of hydroxyproline in connective tissues. Keio J. Med. 27:43–46,1978.

    CAS  PubMed  Google Scholar 

  9. Woessner, J.F. Jr.: The determination of hydroxyproline in tissue and protein samples containing small proportions of this imino acid. Arch. Biochem. Biophys. 93:440–447,1961.

    CAS  PubMed  Google Scholar 

  10. Narukawa,Y., Sato,K., Kato,N., Moriyama,Y. and Fujimura,S.: Collagen synthesis, collagenase activity and adhesiveness with the liver slices in rat osteosarcoma MSK cells. Jpn.J.Cancer Assoc. 48th annual meeting (abstract): 256,1990.

  11. Hosono, T., Takahashi, K., Sato, K. and Fujimura, S.: Bone resorption inducing activity from the conditioned medium of the rat osteosarcoma MSK cells considered as an osteoblastic phenotype. J.Jpn.Stomatol.Soc. 40:398–405,1991.

    Google Scholar 

  12. Hosono,T., Takahashi,K., Sato,K., Moriyama,Y. and Fujimura,S.: An assay method for bone resorption activity using bone chips of45Ca labeled newborn mouse calvaria. Anal.Biochem. (In press),1991.

  13. Majeska, R.J., Rodan, S.B. and Rodan, G.A.: Parathyroid hormone responsive clonal cell lines from rat osteosarcoma. Endocrinology 107:1494–1503,1980.

    CAS  PubMed  Google Scholar 

  14. Hamada, H., Amitani, K., Ono, K., Sakamoto, Y., Kishimoto, T. and Nakata, T.: Osseous alkaline phosphatase from osteosarcoma in mouse and in culture. Cellular & Molecular Biol. 25:77–84,1979.

    CAS  Google Scholar 

  15. Levine, A.M. and Rosenberg, S.A.: Alkaline phosphatase levels in osteosarcoma tissue are related to prognosis. Cancer 44:2291–2293,1979.

    CAS  PubMed  Google Scholar 

  16. Kodama, H., Amagai, Y., Sudo, H, Kasai, S. and Yamamoto, S.: Establishment of a clonal osteogenic cell line from newborn mouse calvaria. Jpn.J.Oral Biol. 23:899–901,1981.

    Google Scholar 

  17. Grigoriadis, A.E., Petkovich, P.M., Ber, R., Aubin, J.E. and Heersche, J.N.M.: (1985) Subclone heterogeneity in a clonally-derived osteoblast like cell line. Bone 6:249–256,1985.

    Article  CAS  PubMed  Google Scholar 

  18. Rodan, S.B., Imai, Y., Thiede, M.A., Wesolowski, G., Thompson, D., Bar Shavit, Z., Shull, S., Mann, K. and Rodan, G.A.: Characterization of a human osteosarcoma cell line (Saos-2) with osteoblastic properties. Cancer Res. 47:4961–4966,1987.

    CAS  PubMed  Google Scholar 

  19. Williams, D.C., Border, G.B., Toomey, R.E., Paul, D.C., Hillman, C.C. Jr., King, K.L., Van Frank, R.M. and Johnston, C.C. Jr.: Mineralization and metabolic response in serially passaged adult rat bone cells. Calcif.Tissue Int. 30:233–246,1980.

    CAS  PubMed  Google Scholar 

  20. Sudo, H., Kodama, H., Amagai, Y., Yamamoto, S. and Kasai, S. In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria. J. Cell Biol. 96:191–198,1983.

    Article  CAS  PubMed  Google Scholar 

  21. Nefussi, J.R., Boy Lefevre, M.L., Boulekbache, H. and Forest, N.: Mineralization in vitro of matrix formed by osteoblasts isolated by collagenase digestion. Differentiation 29:160–168,1985.

    CAS  PubMed  Google Scholar 

  22. Grigoriadis, A.E., Heersche, J.N.M. and Aubin, J.E.: Differentiation of muscle, fat, cartilage, and bone from progenitor cells present in a bone-derived clonal cell population: Effect of dexametnasone. J. Cell Biol. 106:2139–2151,1988.

    Article  CAS  PubMed  Google Scholar 

  23. Rouleau, M.F., Mitchell, J. and Goltzman, D.: In vivo distribution of parathyroid hormone receptors in bone: evidence that a predominant osseous target cell is not the mature osteoblast. Endocrinology 123:187–191,1988.

    CAS  PubMed  Google Scholar 

  24. Takigawa, M., Ishida, H., Takano, T. and Suzuki, F.: Polyamine and differentiation: induction of ornithine decarboxylase by parathyroid hormone is a good marker of differentiated chondrocytes. Proc.Natl.Acad.Sci.USA 77:1481–1485,1980.

    CAS  PubMed  Google Scholar 

  25. Lewinson, D. and Silbermann, M.: Parathyroid hormone stimulates proliferation of chondroprogenitor cells in vitro. Calcif.Tissue Int. 38:155–162,1986.

    CAS  PubMed  Google Scholar 

  26. Grigoriadis, A.E., Aubin, J.E. and Heersche, J.N.M.: Effects of dexamethasone and vitamin D3 on cartilage differentiation in a clonal chondrogenic cell population. Endocrinology 125:2103–2110,1989.

    CAS  PubMed  Google Scholar 

  27. Peck, W.A., Carpenter, J., Messinger, K. and Debra, D.: Cyclic 3′5′ adenosine monophosphate in isolated bone cells: response to low concentration of parathyroid hormone. Endocrinology 92:692–697,1973.

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Central Research Laboratories, Yamanouchi, Pharmaceutical Co., Ltd., 1-8 Azusawa 1-chome, Itabashi-ku, 174, Tokyo, Japan

    Hiroyuki Kawashima

Authors
  1. Hiroyuki Kawashima
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Kawashima, H. Vitamin D and calcium metabolism in hypertension and vascular smooth muscle cell function. J Bone Miner Metab 9, 3–9 (1991). https://doi.org/10.1007/BF02374899

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02374899

Search

Navigation