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Antagonistic effects of soil bacteria onFusarium oxysporum Schlecht f. sp.dianthi (Prill and Del.) Snyd. and Hans

III. Relation between protection against fusarium wilt in carnation and bacterial antagonists colonization on roots

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Summary

Fusarium oxysporum f. sp.dianthi, pathogenic on carnation plants is very sensitive toBacillus subtilis M51 inhibition.Fusarium oxysporum disease (fusariosis) is prevented for a period of two months after treatment of plants withBacillus subtilis M51. The persistence ofB. subtilis M51, marked for selenomycin resistance (MZ51) and inoculated on the roots of carnation cuttings was studied. Soil used was two types: naturally infested withFusarium oxysporum and free from this pathogen. Bacterial cells presence on the roots was detected by direct plating and the presence of the pathogen in the roots was investigated by histological assays. Evidence gathered by these procedures suggest that plant protection is dependent on the physical presence ofB. subtilis M51 cells on the roots.

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References

  1. Aldrich J and Baker R 1970 Biological control ofFusarium roseum f. sp.dianthi byBacillus subtilis. Plant Dis. Rep. 54, 446–448.

    Google Scholar 

  2. Bagnoli G, Filippi C and Picci G 1985 Applicazioni e limiti della batterizzazione di materiale di propagazione vegetale nell'ambito della lotta biologica. XXI Congr. Naz. Microbiol. Roma.

  3. Baker K F and Cook R J 1982 Biological control of plant pathogens Ed Garret S D Amer. Phytopath. Soc., St Paul, Minnesota.

    Google Scholar 

  4. Bowen G D and Rovira A D 1966 Microbial factor in short term phosphate uptake studies with plants. Nature 211, 665–6.

    CAS  Google Scholar 

  5. Broadbent P, Baker F and Waterworth J 1971 Bacterial and actinomycetes antagonistic to fungal root pathogens in Australian soils. Aust. J. Biol. Sci. 24, 925–944.

    CAS  PubMed  Google Scholar 

  6. Broadbent P, Baker K F and Johnston N 1974 Effect ofBacillus spp. on growth of seedlings in steamed soil. Aust. J. Agric. Res.

  7. Brown M E, Burlingham S K and Jackoson R M 1962 Studies onAzotobacter species in soil. II. Population of Azotobacter in the rhizosphere and effects of artificial inoculation plant soil. Plant and Soil 17, 320–332.

    Google Scholar 

  8. Chang J P and Kommedahl T 1968 Biological control of seedling blight of corn by coating kernels with antagonistic microorganisms. Phytopathol. 58, 1395–1401.

    Google Scholar 

  9. Dimock A W 1948 Suggestion for the control of carnation diseases. Carnation craft I, 4, 5–6.

    Google Scholar 

  10. Elad Y, Chet I and Henis Y 1982 Degradation of plant fungi byTrichoderma harzianum. Can. J. Microbiol. 28, 719–725.

    CAS  Google Scholar 

  11. Filippi C, Bagnoli G and Picci G 1983 Effetto antimicotico ad ampio spettro di un batterio del terreno nei riguardi di specie fitopatogene, opportunistiche e saprofite. XX Congr. Naz. Microbiol. Brescia, Gardone Riviera.

  12. Filippi C, Bagnoli G, Treggi G and Picci G 1984 Antagonistic effect of soil bacteria onFusarium oxysporum f. sp.dianthi (Prill and Dell.) Snyd. and Hans. IIn vitro experimental and preliminary assays on carnation (Dianthus caryophyllus L.). Plant and Soil 80, 119–125.

    Article  Google Scholar 

  13. Filippi C, Bagnoli G and Picci G 1985 Effetto antagonista di batteri terricoli suFusarium oxysporum Schlecht f. sp.dianthi (Prill and Del.) Snyd. and Hans. II Effetto antimicotico ad ampio spettro di un batterio del terreno nei riguardi di specie fitopatogene, opportunistiche e saprofite. L'Agr. It. 1/2. 27–40.

    Google Scholar 

  14. Jenny H and Grossembacher K 1963 Root-soil boundary zones as seen in the electron microscope. Proc. Soil Sci. Soc. Am. 27, 273–277.

    Google Scholar 

  15. Kirby W M N, Sherris J C and Tuzch M 1966 Antibiotic susceptibility testing by a standardized single-disc method. Am. J. Clin. Pathol. 45, 439–496.

    Google Scholar 

  16. Lepidi A A, Filippi C, Casella S and Nuti M P 1980 Controllo biologico diFusarium oxysporum Linkin vitro ed in campo su pomodoro, melone e cipolla.

  17. Mankau R 1980 Biological control of nematode pests by natural enemies. Annu. Rev. Phytopathol. 18, 415–440.

    Article  Google Scholar 

  18. Merriman P R, Price R D, Piggot T and Ridge E H 1974 Effect of seed inoculation withBacillus subtilis andStreptomyces griseus on the growth of cereals and carrots. Aust. J. Agric. 25, 219–226.

    Google Scholar 

  19. Mishutin E N and Naumova A N 1962 Bacterial fertilizers, their effectiveness and mode of action. Mikrobiologiya 31, 543–555.

    Google Scholar 

  20. Nair N G and Fahy P C 1972 Bacterial antagonistic toPseudomonas tolaasii and their control of brown blotch of the cultivated mushroomAgaricus bisporus. J. Appl. Bacteriol. 35, 439–442.

    CAS  PubMed  Google Scholar 

  21. Patel J J and Brown M E 1969 Interaction of Azotobacter with rhizosphere root-surface microflora. Plant and Soil 31, 273–281.

    Google Scholar 

  22. Phillips J M and Hayman D S 1970 Improved procedures for clearing roots and staining parasitic and vesicular-arbuscolar mycorrhizal fungi for rapid assessment of infection. Transaction British Mycol. Soc. 55, 158–160.

    Google Scholar 

  23. Ridye E H and Rovira A D 1968 Microbial inoculation of wheat. Trans. Int. Congr. Soil Sci. qth, III, 473–481.

    Google Scholar 

  24. Rovira A D 1965 The effect ofAzotobacter, Bacillus andClostridium on the growth of wheat.In Plant Microbe Relations. Eds. J. Macura and V. Vancura pp 193–200, Czech. Acad. Sci., Prague.

    Google Scholar 

  25. Rovira A D and Bowen G D 1969 Phosphate incorporation by sterile and non-sterile roots. Aust. J. Biol. Sci. 19, 1167–1169.

    Google Scholar 

  26. Tweit M and Wood R K S 1955 The control of Fusarium blight in oat seedlings with antagonistic species of Chaetomium. Ann Appl. Biol., 43, 538–552.

    Google Scholar 

  27. Vrany J, Vancura V and Stanek M 1981 Control of microoganisms in the rhizosphere of wheat by inoculation of seed withPseudomonas putida and by foliar application of urea. Folia Microbiol. 26, 45–51.

    CAS  Google Scholar 

  28. Wood R K S and Tweit M 1955 Control of plant disease by use of antagonistic organisms. Bot. Rev. 21, 441–492.

    Google Scholar 

  29. Wright J M 1956 Biological control of soil-borne Pythium infection by seed inoculation. Plant and Soil 8, 132–140.

    Article  Google Scholar 

  30. Yeh C C and Sinclair J B 1980 Effect ofChaetomium cupreum on seed germination and anagonism to other seedborne fungi of soybean. Plant Disease 64, 468–470.

    Google Scholar 

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Filippi, C., Bagnoli, G., Volterrani, M. et al. Antagonistic effects of soil bacteria onFusarium oxysporum Schlecht f. sp.dianthi (Prill and Del.) Snyd. and Hans. Plant Soil 98, 161–167 (1987). https://doi.org/10.1007/BF02374820

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  • DOI: https://doi.org/10.1007/BF02374820

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