Summary
Five different Brazilian soils were examined for the incidence of Bacillus nitrogen fixers. These counts were set in relation to the total spore count, and the number of facultative anaerobic spores. Twenty-four Bacillus strains were isolated using media lacking a nitrogen source, identified and their acetylene-reducing ability was determined. Eighteen of these strains were identified asB. polymyxa, and the other 6 were related to this species, but were different in some taxonomical tests. Of the 24 isolated strains, only 13 reduced acetylene; similarly, only 3 out of 9B. polymyxa reference strains were able to reduce acetylene.
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References
Abdel Wahab A M 1975 Nitrogen fixation by Bacillus strains isolated from the rhizosphere ofAmmophila arenaria. Plant and Soil 42, 703–708.
Abdel Wahab A M 1980 Characterization of nitrogen-fixing (C2H2-reducing)Bacillus species from Egyptian soils. Z. Allgem. Mikrobiol. 20, 487–494.
Abdel Wahab A M and El-Sharouny H M 1979 Nitrogen-fixingBacillus species from Egyptian soils: acetylene reduction and cultural conditions. Plant and Soil 51, 187–196.
Alexander M 1977 Introduction to Soil Microbiology, 2nd ed., pp. 16–35, John Wiley & Sons, New York.
Beijerinck M W 1901 Ueber oligonitrophile Mikroben. Zbl. Bakteriol. Parasitenkd. Abt. II, 7, 561–564.
Bredemann G 1908 Untersuchungen ueber die Variation und das Stickstoffbindungsvermogen desBacillus asterosporus A. M. ausgefuehrt an 27 Staemmen verschiedener Herkunft. Zbl. Bakteriol. Parasitenkd. Abt. II, 22, 44–89.
Dalton H 1974 Fixation of dinitrogen by free-living microorganisms. CRC Crit. Rev. Microbiol. 3, 183–220.
Elsas J D van, Seldin L and Penido E G C 1978 Ecology and taxonomy of Bacillus in Brazilian soils. An. Microbiol. Rio de Janeiro 23, 59–78.
Gibson T and Gordon R E 1975 The Genus Bacillus.In Bergey's Manual of Determinative Bacteriology (8th ed.), pp 529–550. Eds. R E Buchanan and N E Gibbons. Williams and Wilkins, Baltimore.
Gordon R E, Haynes W C and Pang H-N 1973 The genus Bacillus. Agric. Handbook 427, Agric. Res. Service, US Dept. of Agric., Washington, D.C.
Greenland D J and Kowal J M L 1960 Nutrient content of the moist tropical forests of Ghana. Plant and Soil 12, 154–174.
Hagler A N 1978 Ecologia e taxonomia de leveduras em um estuário poluido e ambientes marinhos do Rio de Janeiro. Ph.D. thesis, Institute of Microbiology, Federal University of Rio de Janeiro.
Hammann R and Ottow J C G 1976 On N2-fixing Clostridia and Bacilli from soils. Zbl. Bakteriol. Hyg., I Abt. Orig. B 161, 527–533.
Hino S and Wilson P W 1958 Nitrogen-fixation by a facultative Bacillus. J. Bacteriol. 75, 405–408.
Jordan D C, McNicol P J and Marshall M R 1978 Biological nitrogen fixation in the terrestrial environment of a high Arctic ecosystem (Truelove Lowland, Devon Island, N.W.T.). Can. J. Microbiol. 24, 643–649.
Jurgensen M F 1973 Relationship between nonsymbiotic nitrogen fixation and soil nutrient status — a review. J. Soil. Sci. 24, 512–522.
Jurgensen M F and Davey C B 1971 Nonsymbiotic nitrogen-fixing micro-organisms in forest and tundra soils. Plant and Soil 34, 341–356.
Larson R I and Neal J L 1978 Selective colonization of the rhizosphere of wheat by nitrogen-fixing bacteria.In Environmental Role of Nitrogen-fixing Blue-green Algae and Asymbiotic Bacteria. Ecol. Bull. (Stockholm) 26, 331–342.
Line M A and Loutit M W 1971 Non-symbiotic nitrogen-fixing organisms from New Zealand tussock-grassland soils. J. Gen. Microbiol. 66, 309–318.
Line M A and Loutit M W 1973 Studies on non-symbiotic nitrogen fixation in New Zealand tussock-grassland soils. N.Z.J. Agric. Res. 16, 87–94.
Marmur J 1961 A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol. 3, 208–218.
Marmur J and Doty P 1962 Determination of base composition of deoxyribonucleic acid from its thermal denaturation temperature. J. Mol. Biol. 5, 109–118.
Mendez W, Cruz Lemos P O, Costa Lemos R, Carvalho L G O and Rosenburg R J 1954 Contribuição ao mapeamento, em séries, dos solos do Município de Itaguaí. Bol. Inst. Ec. Exp. Agric. 12, 5–53.
Mishustin E N and Mirsoeva V A 1968 Sporeforming bacteria in the soils of the USSR.In The Ecology of Soil Bacteria. pp 458–473. Eds. T R G Gray and D Parkinson. Liverpool University Press.
Moore A W 1963 Nitrogen fixation in latosolic soil under grass. Plant and Soil 19, 127–138.
Moore A W and Becking J H 1963 Nitrogen fixation by Bacillus strains isolated from Nigerian soils. Nature London 4883, 915–916.
Pramer D and Schmidt E L 1965 Experimental Soil Microbiology. Burgess Publ. Comp., Minnesota.
Siala A, Hill I L and Gray T R G 1974 Populations of sporeforming bacteria in an acid forest soil with special reference toBacillus subtilis. J. Gen. Microbiol. 81, 183–190.
Winogradsky S 1895 Recherches sur l'assimilation de l'azote libre de l'atmosphère par les microbes. Arch. Sci. Biol. 4, 297–352.
Witz D F, Detroy R W and Wilson P W 1967 Nitrogen fixation by growing cells and cell-free extracts of the Bacillaceae. Arch. Mikrobiol. 55, 369–381.
Wullstein L H, Bruening M L and Bollen W B 1979 Nitrogen fixation associated with sand grain root sheaths (rhizosheaths) of certain xeric grasses. Physiol. Plant. 46, 1–4.
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Seldin, L., Van Elsas, J.D. & Penido, E.G.C. Bacillus nitrogen fixers from Brazilian soils. Plant Soil 70, 243–255 (1983). https://doi.org/10.1007/BF02374784
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DOI: https://doi.org/10.1007/BF02374784