Journal of Bone and Mineral Metabolism

, Volume 8, Issue 1, pp 10–16 | Cite as

Effect of 1α-hydroxyvitamin D3 on bone metabolic disorders in gastrectomized rats

  • Hiroshi Uno
  • Yoshihiro Izawa
  • Kiyoshi Sagara
  • Tamotsu Koyama
  • Tokutaro Makita
  • Chia-Yuan Hsu-Chang
  • Mitsugi Sugiyama
Original Articles

Abstract

The effect of 1α-hydroxyvitamin D3 (1α(OH)D3) on the metabolic bone disorders developed in gastrectomized rats were investigated biochemically and histomorphologically. 1α(OH)D3 was suspended in 0.2 % Triton-X-100 aqueous solution after dissolving in a very small amount of ethanol, was given orally to the rats for 10 weeks. The sham operated animals and the gastrectomy control animals received the vehicle alone. Gastrectomy was followed by the development of the metabolic bone disorders after 10 weeks of observation. This was characterized by reduction in ash content of the femur and histologically by a disappearance of the trabecular bone in tibial metaphysis. Decrease Ca absorption from the intestines was demonstrated by a radiotracer technique. Biochemical studies showed significant decreases in serum 25(OH)D concentration in gastrectomized rats. These findings suggest that gastrectomy partially impairs intestinal absorption of calcium and results in a negative calcium balance, which may contribute to the development of bone metabolic disorders in rats. The administration of 1α(OH)D3 increased dose-dependently serum calcium and Ca absorption from the intestine and prevented the development of bone metabolic disorders histomorphologically.

Key Words

Gastrectomy Bone Metabolic Disorder 1α-hydroxy vitamin D3 

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References

  1. 1.
    Sarasin, C.: Osteomalacie und hypochrome anaemie nach magenresektion. Gastroenterologia 66: 182–197, 1941.Google Scholar
  2. 2.
    Thompson, G.R., Lewis, B. and Booth, C.C.: Vitamin D absorption after partial gastrectomy. Lancet 1: 457–458, 1966.PubMedGoogle Scholar
  3. 3.
    Taylor, C.M., Hughes, S.E. and Desilva, P.: Competitive protein binding assay for 24,25 dihydroxycholecalcififerol. Biochem. Biophys. Res. Commun. 70: 1243–1249, 1976.PubMedGoogle Scholar
  4. 4.
    Eisman, J.A., Hamstra, A.J., Kream, B.E. and Deluca, H.: A sensitive precise and convenient method for determination of 1,25-dihydroxyvitamin D in human plasma. Arch. Biochem. Biophys. 176: 235–243, 1976.CrossRefPubMedGoogle Scholar
  5. 5.
    Miyada, D.S. and Tappel, A.L.: Colorimetric determinarion of hydroxyproline. Anal. Chem. 28: 909–910, 1956.CrossRefGoogle Scholar
  6. 6.
    Comfort, M.W., Wollaeger, E.E. and Taylor, A.B.: Nontropical sprue: observations on absorption and metabolism. Gastroenterology 23: 155–178, 1953.PubMedGoogle Scholar
  7. 7.
    Harvald, B., Krogsgaard, A.R. and Lous, P.: Calcium deficiency following partial gastrectomy. Acta. Med. Scand. 172: 497–503, 1962.PubMedGoogle Scholar
  8. 8.
    Thompson, G.R., Lewis, B. and Booth, C.C.: Absorption of Vitamin D3-3H in control subjects and patients with intestinal malabsorption. Clin. Invest. 45: 94–102, 1966.Google Scholar
  9. 9.
    Imawari, M., Kozawa, K., Akanuma, Y., Koizumi, S., Itakura, H. and Kosaka, K.: Serum 25-hydroxyvitamin D and vitamin D - binding protein levels and mineral metabolism after partial and total gastrectomy. Gastroenterology 79: 255–258, 1980.PubMedGoogle Scholar
  10. 10.
    Hirota, M.: A clinical and histological evaluation of bone disease following total gastrectomy. Jpn. J. Gastroenterol Surg. 15: 1716–1727, 1982.Google Scholar
  11. 11.
    Nilas, L. and Christiansen, C.: Influence of PTH and 1,25(OH)2D on calcium homeostasis and bone mineral content after gastric surgery. Calcif. Tissue Int. 37: 461–466, 1985.PubMedGoogle Scholar
  12. 12.
    Eastwood, J.B. and Wardener, H.E.: Normal plasma 1,25(OH)2D concentrations in nutrional osteomalacia. Lancet 1: 1377–1378, 1979.PubMedGoogle Scholar
  13. 13.
    Deluca, H.E.: The kidney as an endocrine organ for the production of 1,25-dihydroxyvitamin D3, a calcium-mobilizing hormone. N. Engl. J. Med. 289: 359–365, 1973.PubMedGoogle Scholar
  14. 14.
    Norman, A.W. and Wong, R.G.: Biological activity of the vitamin D metabolie 1,25 dihydroxycholecalciferol in chickens and rats. J. Nutr. 102: 1709–1718, 1972.PubMedGoogle Scholar
  15. 15.
    Raisz, L.G., Trummel, C. L., Holick, M. F. and Deluca, H.F.: 1,25-dihydroxycholecalciferol: A potent stimulator of bone resorption in tissue culture. Science 75: 768–769, 1972.Google Scholar

Copyright information

© Japanese Society of Bone Metabolism Research 1990

Authors and Affiliations

  • Hiroshi Uno
    • 1
  • Yoshihiro Izawa
    • 1
  • Kiyoshi Sagara
    • 1
  • Tamotsu Koyama
    • 1
  • Tokutaro Makita
    • 1
  • Chia-Yuan Hsu-Chang
    • 2
  • Mitsugi Sugiyama
    • 2
  1. 1.2ND Bio-medical Research Laboratories, Teijin LimitedHiroshi UNOTokyoJapan
  2. 2.Second Department of SurgeryYokohama City University School of MedicineYokohamaJapan

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