The effect of leaf exudates on the spore germination of phylloplane mycoflora of chilli (Capsicum annuum L.) cultivars


Ninetten aminoacids, twelve sugars, eleven organic acids and ten phenols were detected in the leaf exudates of three cultivars of chilli. The number of aminoacids, sugars, organic acids and phenols increased as the plants grew older. More aminoacids and sugars were detected in the exudate from the susceptible cultivar (Malwa). More organic acids and phenols were detected from the resistant cultivar (Simla). The leaf exudate of the resistant cultivar (Simla) inhibited spore germination of the pathogen (Alternaria solani) while that of susceptible (Malwa) stimulated spore germination. The cultivar ‘Patna’ which is moderately resistant, occupied an intermediate position.

Spore germination of the isolated fungi was enhanced in leaf exudate of susceptible cultivar (Malwa), while leaf exudates of the moderately resistant (Patna) and resistant (Simla) inhibited spore germination of the majority of fungi isolated. Most of the antagonistic fungi were not isolated from the susceptible cultivar and the percentage spore germination of these fungi was less in leaf exudate of the susceptible cultivar, while leaf exudates of resistant cultivars enhanced the percentage spore germination of antagonistic fungi,viz Aspergillus flavus, A. fumigatus, A. versicolor, Penicillium citrinum, P. restrictum andTrichoderma viride.

This is a preview of subscription content, access via your institution.


  1. 1

    Afifi A F 1975 Effect of volatile substances from sp. of Labiatae on rhizosphere and phyllosphere fungi ofPhaseolus vulgaris. Phytopathol. Z. 83, 296–305.

    Google Scholar 

  2. 2

    Brillova D 1971 Influence of phenolics substances on the germination of conidia ofCercospora beticola Sacc. Biologia (Bratislava) 26, 713–723.

    Google Scholar 

  3. 3

    Carrasco A, Boudet A M and Marigo G 1978 Enhanced resistance of tomato plants to Fusarium by controlled stimulation of their natural phenolic production. Physiol. Plant Pathol. 12, 225–232.

    CAS  Google Scholar 

  4. 4

    Dix N J 1974 Identification of water soluble fungal inhibitors in the leaves ofAcer plantanoides L. Ann. Bot. 38, 504–514.

    Google Scholar 

  5. 5

    Edreva Aglika 1976 The significance of phenols in plant resistance phenomenon. Gen. Sci. 9, 150–159.

    CAS  Google Scholar 

  6. 6

    Godfrey B E S and Clements D M 1978 Effect of Lilac leaf leachates on the germination ofAlternaria alternata andBotrytis cinerea. Trans. Br. Mycol. Soc. 70, 163–165.

    Google Scholar 

  7. 7

    Hadwiger L A and Hall C V 1961 The relationship of pigmentation and free aminoacids content with resistance toColletotrichum lagenasium in watermelon. Plant Dis. Rep. 45, 373–374.

    CAS  Google Scholar 

  8. 8

    Irvine J A and Dix N J 1978 Inhibitory substances inAcer platanoides leaves: seasonal activity and effects on growth of phylloplane fungi. Trans. Br. Mycol. Soc. 70, 363–371.

    CAS  Google Scholar 

  9. 9

    Kuc J, Henze R E, Ullstrup A J and Quackenbush F W 1956 Chlorogenic acid, Caffeic acid as fungistatic agents produced by potatoes in response to inoculation withHelminthosporium carborum. J. Am. Chem. Soc. 78, 3123–3125.

    CAS  Article  Google Scholar 

  10. 10

    Mace M E and Veech J A 1973 Inhibition ofHelminthosporium turcium spore germination by leaf diffusates from northern leaf blight susceptible or resistant corn. Phytopathology 63, 1393–1394.

    Google Scholar 

  11. 11

    Miller R L 1962 Stimulatory effect of spore germination. Source book of laboratory exercise in plant pathology. Am. Phytopathol. Soc. 50 p.

  12. 12

    Purkayastha R P and Mukhopadhyay R 1974 Factors affecting the colonization of leaves byHelminthosporium oryzae. Trans. Br. Mycol. Soc. 62, 402–406.

    Google Scholar 

  13. 13

    Randernath K 1963 Thin layer chromatography. Academic Press, New York.

    Google Scholar 

  14. 14

    Schneider R W and Sinclair J B 1975 Inhibition of conidial germination and germ tube growth ofCercospora canescens by cowpea leaf diffusates. Phytopathology 65, 63–65.

    Google Scholar 

  15. 15

    Topps J H and Wain R L 1957 Fungistatic properties of leaf exudates. Nature London 179, 652–653.

    CAS  PubMed  Google Scholar 

  16. 16

    Tyagi V K 1980 Ecology of leaf surface micro-organisms in relation to certain fungal disease of vegetables. Ph.D. Thesis, Vikram University, Ujjain, India.

    Google Scholar 

Download references

Author information



Rights and permissions

Reprints and Permissions

About this article

Cite this article

Tyagi, V.K., Chauhan, S.K. The effect of leaf exudates on the spore germination of phylloplane mycoflora of chilli (Capsicum annuum L.) cultivars. Plant Soil 65, 249–256 (1982).

Download citation

Key words

  • Capsicum annuum
  • Chilli
  • Leaf exudates
  • Inhibition
  • Phylloplane
  • Stimulation