Plant and Soil

, Volume 63, Issue 2, pp 299–302 | Cite as

‘Chillum’ jar, a better technique for screening of rhizobia under summer conditions

  • J. S. Dahiya
  • A. L. Khurana
Short Communications


In the present investigations ‘Chillum’ jar assembly was found to provide more favourable environmental conditions for rhizobia to nodulate leguminous plants particularly under summer conditions than the usual Leonard jar assembly. When thirty pigeon pea rhizobia isolates were tested for their nodulation efficiency in both Leonard jars as well as ‘Chillum’ jars, it was noticed that there was no nodulation in any of the isolates under Leonard jars whereas all isolates were nodulating well under ‘Chillum’ jars conditions. This was probably due to lowering of temperature in ‘Chillum’ jar caused by rapid evaporation from the outer surface of ‘Chillum’ jar assembly. The maximum temperature recorded in ‘Chillum’ jar was 34°C whereas in Leonard jars it was 46.5°C.

Key Words

‘Chillum jar’ Pigeon pea Rhizobia 


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Literature cited

  1. 1.
    Dart, P. al. 1975 First International Workshop on Grain Legumes, held at ICRISAT, Hyderabad, India, Jan. 13–16, pp 63–85.Google Scholar
  2. 2.
    Jensen, H. L. 1942 Proc. Linn. Soc. N.S.W.66, 98–108.Google Scholar
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    Leonard, L. T. 1943 J. Bacteriol.45, 523–525.PubMedGoogle Scholar
  4. 4.
    Vincent, J. M. 1971In A Manual for Practical Studies of the Root Nodule Bacteria. p. 77 Blackwell Scientific Publication, Oxford and Edinburgh.Google Scholar

Copyright information

© Martinus Nijhoff/Dr W. Junk Publishers 1981

Authors and Affiliations

  • J. S. Dahiya
    • 1
  • A. L. Khurana
    • 1
  1. 1.Department of MicrobiologyHaryana Agricultural UniversityHissarIndia

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