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Targeted retroviral gene transfer into the rat biliary tract

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Somatic Cell and Molecular Genetics

Abstract

The ability to induce proliferation by temporary duct ligation suggested an hypothesis that retrovirus-mediated gene transfer into cells of the biliary tract could be accomplished. The time course of histologic changes, incorporation of3H-thymidine and immunofluorescent staining with a monoclonal antibody to cytokeratin-19 (a marker for differentiated bile ducts) was studied in male Fischer F344 rats. A recombinant Gibbon ape leukemia virus (GALV), containing a gene encodingEscherichia coli β-galactosidase was next introduced into 24 hr obstructed bile ducts. Gene transfer was maximal when virus was exposed to the obstructed duct for 12 hr (∼0.1%). The majority of X-gal positive cells were in cytokeratin-19 negative peribiliary tissues, which had the appearance of newly forming bile ducts. The data suggest that cells targeted by retroviral infection of the obstructed rat bile duct may be a precursor of mature, fully differentiated biliary epithelium.

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Authors and Affiliations

  1. University of Michigan Medical School, Room 206, Wistar Institute, 36th and Spruce Streets, 19104-4268, Philadelphia, Pennsylvania

    Jesus A. Cabrera

  2. Institute for Human Gene Therapy, Room 206, Wistar Institute, 36th and Spruce Streets, 19104-4268, Philadelphia, Pennsylvania

    Steven E. Raper

  3. Department of Molecular and Cellular Engineering, Department of Surgery, University of Pennsylvania School of Medicine, Pennsylvania, USA

    James M. Wilson

Authors
  1. Jesus A. Cabrera
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  2. James M. Wilson
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  3. Steven E. Raper
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Cabrera, J.A., Wilson, J.M. & Raper, S.E. Targeted retroviral gene transfer into the rat biliary tract. Somat Cell Mol Genet 22, 21–29 (1996). https://doi.org/10.1007/BF02374373

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  • DOI: https://doi.org/10.1007/BF02374373

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