Plant and Soil

, Volume 62, Issue 2, pp 265–278 | Cite as

Differences in nitrate uptake of species from habitats rich or poor in nitrogen when grown at low nitrate concentrations, using a new growth technique

  • Siebren J. Van De Dijk
Article

Summary

A method is described for culturing plants at extremely low nutrient concentrations. Using a Braun infusion pump, a fixed amount of nitrate or ammonium was supplied continuously to plants growing in a culture vessel at a rate limiting the uptake of the plants. At a very low nitrogen concentration an equilibrium was established where uptake rate of the plants is equal to the rate of supply by the infusion pump. The nitrogen concentrations reached appeared to be in the order of 1 μM.

The method compared the nitrate uptake byHypochaeris radicata L.ssp.radicata, H. radicata ssp.ericetorum Van Soest andUrtica dioica L. and ammonium uptake byH. radicata ssp.radicata andH. radicata ssp.ericetorum. Plants were cultivated in monocultures or in mixed cultures (two species per culture vessel). For the mixed cultures competition for nitrate (or ammonium) between the species was maintained for long periods. The capacities of the uptake systems of two subspecies ofH. radicata from places different in nitrogen supply and pH were adapted equally well to both low nitrate and low ammonium concentrations.

Apparently factors other than nitrogen uptake play a part in the distribution of the subspecies. The capacity of the uptake system ofU. dioica, a nitrophilous species, was lower than that ofH. radicata ssp.radicata, a species from places poorer in nitrogen. This difference is related to the different distribution of the two species in the field.

The present results are compared with those of previous experiments where Km and Vmax were measured and the significance of both parameters is discussed.

Key Words

Competition Ecophysiology Hypochaeris radicata Nitrate Nitrate uptake Urtica dioica 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Chapin, F. S. III 1980 The mineral nutrition of wild plants. Annu. Rev. Ecol Syst.11, 233–260.CrossRefGoogle Scholar
  2. 2.
    Clarkson, D. T. and Hanson, J. B. 1980 The mineral nutrition of higher plants. Annu. Rev. Plant Physiol.31, 239–298.CrossRefGoogle Scholar
  3. 3.
    Clement, C. R., Hopper, M. J. and Jones, L. H. P. 1978 The uptake of nitrate byLolium perenne from flowing nutrient solution. I. Effect of nitrate concentration. J. Exp. Bot.29, 453–464.Google Scholar
  4. 4.
    Freysen, A. H. J. and Otten, H. 1979 The growth and nitrate uptake ofPlantago lanceolata andP. major in experiments of 750 and 25 μM. Verh. K. Ned. Akad. Wet. Afd. Natuurkd. II,73, 364–367.Google Scholar
  5. 5.
    Freysen, A. H. J. and Otten, H. 1981 Nitrate uptake and growth ofPlantago lanceolata L. andPlantago major L. at the continuous and intermittent supply of low concentrations of nitrate.In preparation.Google Scholar
  6. 6.
    Heukels, H. and Van Ooststroom, S. J. 1968 Flora van Nederland. Wolters-Noordhoff B. V. Groningen, 15th. edition.Google Scholar
  7. 7.
    Hoagland, D. R. and Snijder, W. C. 1933 Nutrition of strawberry plant under controlled conditions. Proc. Am. Soc. Hortic. Sci.30, 288–296.Google Scholar
  8. 8.
    Humphries, E. C. 1956 Mineral components and ash analysis.In Modern Methods of Plant Analysis I, pp 481–483. Eds. Peach and Tracey.Google Scholar
  9. 9.
    Ingestad, T. and Lund, A.-B. 1979 Nitrogen stress in birch seedlings. I. Growth technique and growth. Physiol. Plant45, 137–148.Google Scholar
  10. 10.
    Lambers, H., Posthumus F., Stulen, I., Lanting, L., Van de Dijk, S. J. and Hofstra, R. 1981 Energy metabolism ofPlantago lanceolata as dependent on the supply of mineral nutrients. Physiol. Plant.51, 85–92.Google Scholar
  11. 11.
    Lambers, H., Posthumus, F., Stulen, I., Lanting, L., Van de Dijk, S. J. and Hofstra, R. 1981 Energy metabolism ofPlantago major ssp.major as dependent on the supply of mineral nutrients. Physiol. Plant.in press.Google Scholar
  12. 12.
    Lewis, J. C. and Powers, W. L. 1941 Antagonistic action of chlorides on the toxicity of ioides to corn. Plant Physiol.16, 393–398.Google Scholar
  13. 13.
    Stulen, I., Lanting, L., Lambers, H., Posthumus, F., Van de Dijk, S. J. and Hofstra, R. 1981 Nitrogen metabolism ofPlantago lanceolata as dependent on the supply of mineral nutrients. Physiol. Plant.51, 93–98.Google Scholar
  14. 14.
    Stulen, I., Lanting, L., Lambers, H., Posthumus, F., Van de Dijk, S. J. and Hofstra, R. 1981 Nitrogen metabolism ofPlantago major L. ssp.major as dependent on the supply of mineral nutrients. Physiol. Plant.In press.Google Scholar
  15. 15.
    Stulen, I., Lambers, H., Van de Dijk, S. J., Posthumus, F., Lanting, L. and Hofstra, R. 1981 Nitrogen and energy metabolism ofUrtica dioica L. as dependent on the supply of mineral nutrients.In preparation.Google Scholar
  16. 16.
    Van de Dijk, S. J. 1980 Two ecological distinct subspecies ofHypochaeris radicata L. I.Nitrate uptake and nitrate utilization. Plant and Soil56, 465–473.CrossRefGoogle Scholar
  17. 17.
    Van de Dijk, S. J. 1980 Two ecologically distinct subspecies ofHypochaeris radicata L. II. Growth response to nitrate and ammonium growth strategy and formative aspects. Plant and Soil57, 111–122.Google Scholar
  18. 18.
    Van de Dijk, S. J. and Troelstra, S. R. 1980 Heterotrophic nitrification in a heath soil demonstrated by anin situ method. Plant and Soil57, 11–21.Google Scholar
  19. 19.
    Van de Dijk, S. J., Lanting, L., Lambers, H., Posthumus, F., Stulen, I. and Hofstra, R. 1981 Kinetics of nitrate uptake by different species, from nutrient-rich and nutrient-poor habitats as affected by the nutrient supply. Physiol. Plant.Submitted.Google Scholar
  20. 20.
    Van de Dijk, S. J., Stulen, I., Lanting, L., Lambers, H., Posthumus, F. and Hofstra, R. 1981 Nitrogen and energy metabolism of two subspecies ofH. radicata as dependent on the supply of mineral nutrients.In preparation.Google Scholar

Copyright information

© Martinus Nijhoff| Dr. W. Junk Publishers 1981

Authors and Affiliations

  • Siebren J. Van De Dijk
    • 1
  1. 1.Department of Plant PhysiologyBiological CentreA A HarenThe Netherlands

Personalised recommendations