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Biological activity of five strains ofMetarhizium anisopliae, upon the coffee berry borerHypothenemus hampei (Col.: Scolytidae)

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Abstract

Five strains of the entomopathogenic fungusMetarhizium anisopliae (Metschnikoff) Sorokin were bioassayed against the coffee berry borerHypothenemus hampei (Ferrari) (Coleoptera: Scolytidae) under laboratory conditions (T=27±2°C; R.H.=90±5% and 12∶12 L∶D light cycle). The LC50 values for the three most virulent strains, Ma4, Ma5 and Ma3, were 4.2, 5.9 and 6.7×106 conidia/ml of suspension respectively and median lethal times (LT50) observed were between 9.7 and 13.8 days. The maximum percentage sporulation was obtained with strains Ma3 and Ma4 both with 90% sporulation and Ma10 with 60.7%. These results suggest that the fungusM. anisopliae could be incorporated into an integrated control programme for the biological control ofH. hampei.

Résumé

Cinq souches du champignon entomopathogèneMetarhizium anisopliae (Metschnikoff) Sorokin, ont été évaluées dans les conditions du laboratoire, pour mesurer leur activité vis-à-vis du scolyte des fruits du caféierHypothenemus hampei (Ferrari) (Coleoptera: Scolytidae). Les résultats montrent que les souches Ma4, Ma5 et Ma3 sont les plus virulentes contreH. hampei, avec des valeurs de concentration létale moyenne (CL50, valeurs minimales et maximales, ± limite de confiance à 95%) de 0,0226 (0,0195–0,00260), 0,0328 (0,0267–0,0390) et 0,0354 (0,0297–0,0417) équivalentes à 4,2, 5,9 et 6,7×106 conidies/ml de suspension et un temps létal moyen (TL50) entre 9,7 et 13,8 jours. Les pourcentages maximaux de sporulation ont été obtenus avec les souches Ma3 et Ma4 avec 90%, la souche Ma10 présentant le pourcentage le plus bas de sporulation (67%). Ces résultats démontrent queM. anisopliae peut être introduit dans un programme de lutte intégrée contreH. hampei.

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Authors and Affiliations

  1. El Colegio de la Frontera Sur (ECOSUR), Carretera Antiguo Areopuerto Km. 2.5., Apdo Postal 36, 30700, Tapachula, Chiapas, México

    W. De La Rosa-Reyes & J. L. Godinez-Aguilar

  2. Instituto de Fitosanidad, Colegio de Postgraduados, 56230, Montecillo, Edo. de México, México

    R. Alatorre-Rosas

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  1. W. De La Rosa-Reyes
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  2. J. L. Godinez-Aguilar
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  3. R. Alatorre-Rosas
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De La Rosa-Reyes, W., Godinez-Aguilar, J.L. & Alatorre-Rosas, R. Biological activity of five strains ofMetarhizium anisopliae, upon the coffee berry borerHypothenemus hampei (Col.: Scolytidae) . Entomophaga 40, 403–412 (1995). https://doi.org/10.1007/BF02373728

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