, Volume 37, Issue 3, pp 347–351 | Cite as

Inapparent infection ofDiatraea grandiosella byHeliothis virescens cytoplasmic polyhedrosis virus

  • P. P. Sikorowski
  • R. G. Luttrell
  • A. M. Lawrence


The terminal stage of infection with cytoplasmic polyhedrosis viruses (CPVs) is formation of crystal-like inclusion bodies (polyhedra) in host insects. The degree of susceptibility of larvae to CPV, based on light microscopy and presence of polyhedra, varies with the host species.Heliothis virescens (F.) andSpodoptera exigua (Hübner) are highly susceptible to CPV. In CPV treatedDiatraea grandiosella (Dyar), polyhedra were absent in all 400 + insects examined with light and electron microscopy. However,H. virescens larvae became infected when fed haemolymph ofD. grandiosella larvae or pupae (36±10 days post treatment) developed from CPV-treated larvae. No difference in pathology was observed betweenH. virescens larvae infected with CPV polyhedra and haemolymph fromD. grandiosella.

This study provides evidence thatD. grandiosella can serve as a symptomless (no occlusion bodies) carrier of a CPV which is fully expressed inH. virescens species. The observation is interesting because it reveals a potentially important aspect of the epizootiology of this insect virus.


Heliothis virescens cytoplasmic polyhedrosis virus inapparent infection Diatreae grandiosella 


Cette étude montre la présence de virions de la polyédrose cytoplasmique (CPV) d'Heliothis virescens dans l'hémolymphe dediatraea grandiosella qui est de ce fait considéré comme un porteur sain du virus. Chez des hôtes considérés comme normaux, tels queH. virescens etSpodoptera exigua, la virose à CPV d'Heliothis virescens se traduit par la présence de nombreux polyèdres intestinaux, tandis que chezD. grandiosella ces polyèdres sont absents des larves virosées. L'hémolymphe des larves deD. grandiosella contaminées est infectieurx par voie orale pour les chenilles d'H. virescens, et deS. exigua chez lesquelles elle induit une virose classique.


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Copyright information

© Lavoisier Abonnements 1992

Authors and Affiliations

  • P. P. Sikorowski
    • 1
  • R. G. Luttrell
    • 1
  • A. M. Lawrence
    • 1
  1. 1.Department of EntomologyMississippi State UniversityMississippi StateUSA

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