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Interference between two nuclear polyhedrosis viruses of the armyworm,Pseudaletia unipuncta [Lep.: Noctuidae]

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Abstract

The larva ofPseudaletia unipuncta (Haworth) is susceptible to 2 nuclear polyhedrosis viruses (NPV), the typical (TNPV) and the hypertrophy (HNPV) strains. The interference between the 2 viruses was studied primarily in the tracheal and fat tissues. When both viruses were microfed simultaneously, no larva had mixed virus infections and most were infected with TNPV. In order to obtain 50% infection by each virus the inoculum of HNPV had to be increased by 1000 polyhedra/ml at the LD50 value of TNPV. Mixed infection resulted when TNPV was inoculated 1 and especially 2 days after HNPV inoculation. In mixed infection, each virus attacked certain areas of the tracheae instead of random cells. These results indicated an interference or antagonism between the 2 viruses. The possible cause for the interference is discussed.

Résumé

La larve dePseudaletia unipuncta (Haworth) est sensible à 2 virus à polyèdres nucléaires (NPV), la souche typique (TNPV) et la souche causant une hypertrophie (HNPV). L'interférence entre ces 2 virus a été étudiée essentiellement dans les trachées et le corps adipeux. Si les deux virus sont inoculés simultanément par micro ingestion aucune larve ne présente d'infection mixte et la plupart d'entre elles est infectée par le TNPV. Pour obtenir 50% d'infection par chacun des virus, l'inoculum de HNPV doit être accru de 1000 polyèdres par ml par rapport à l'inoculum provoquant la DL 50 du TNPV. Une infection mixte est enregistrée quand le TNPV est inoculé 1 jour et de préférence 2 jours après l'introduction du HNPV. Dans ce cas d'infection mixte chaque virus attaque certaines régions des trachées et non pas des cellules réparties au hasard. Ces résultats prouvent une interférence ou un antagonisme entre les 2 virus, dont la cause est discutée.

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Authors and Affiliations

  1. Division of Entomology and Parasitology, University of California, 94720, Berkeley, CA, USA

    Karla S. Ritter & Y. Tanada

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  1. Karla S. Ritter
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  2. Y. Tanada
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Portion of a dissertation submitted by the senior author for the degree of doctor of philosophy from the University of California, Berkeley. Part of the study was supported by a NSF grant provided to the junior author.

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Ritter, K.S., Tanada, Y. Interference between two nuclear polyhedrosis viruses of the armyworm,Pseudaletia unipuncta [Lep.: Noctuidae] . Entomophaga 23, 349–359 (1978). https://doi.org/10.1007/BF02373052

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