, Volume 39, Issue 3–4, pp 351–361 | Cite as

Metarhizium spp. isolates from madagascar: Morphology and effect of high temperature on growth and infectivity to the migratory locust,Locusta migratoria

  • M. Welling
  • G. Nachtigall
  • G. Zimmermann


Five strains ofMetarhizium anisopliae (Metsch.) Sorokin and one strain ofMetarhizium flavoviride Gams & Rozsypal originally isolated in Madagascar were studied. Measurements of conidia and, for the first time, also of blastospores produced in a liquid medium were used for species and variety determination. Blastospores ofM. flavoviride were more homogenous in their size than those ofM. anisopliae. Growth at high temperatures between 25° and 40°C showed that 4 isolates ofM. anisopliae grew at 36°C andM. flavoviride grew at 38°C. Using alternating day/night temperatures (8/16 h) the three strains tested could also tolerate 40°/25°C. In bioassays, fiveMetarhizium spp. isolates were tested against third and fourth instar larvae ofLocusta migratoria (L.) at two alternating day/night temperatures of 30°/25°C and 36°/25°C. In the cooler regime, all strains caused a mortality of 50% within 5.9 to 8.5 days (median lethal time), while in the 36°/25°C treatment only the thermophilicM. flavoviride and oneM. anisopliae strain isolated from a soil sample gave comparable results with median lethal times of 6.8 and 7.3 days, respectively.


Metarhizium anisopliae Metarhizium flavoviride morphology temperature Locusta migratoria Madagascar 


Les auteurs ont étudié cinq souches deMetarhizium anisopliae et une souche deM. flavoviride isolées à Madagascar. Les blastospores de la seconde espèce apparaissent plus homogènes de taille que celles deM. anisopliae. Quatre des 5 souches de cette espèce peuvent croître à 36°C tandis que l'isolat deM. flavoviride se développe à 38°C (températures constantes). Cette souche ainsi que deux souches deM. anisopliae sont capables de croître à des températures de 40°C (pendant 8 h) alternant avec des températures de 25°C (pendant 16 h). Dans les essais d'infection de stades L3/L4 deLocusta migratoria à une température de 30°C (pendant 8 h) alternant avec une température de 25°C (pendant 16 h), les 4 souches deM. anisopliae et la souche deM. flavoviride ont toutes donné une mortalité de 50% en 5,9–8,5 jours. Dans le cas où la température est de 36° au lieu de 30°C (pendant 8 h), seule la souche deM. flavoviride et une souche deM. anisopliae isolée du sol ont donné des mortalités élevées.


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  1. Adamek, L. — 1963. Submerse cultivation of the fungusMetarhizium anisopliae (Metsch.). —Folia Microbiologia (Praha), 10, 255–257.Google Scholar
  2. Bateman, R.P., Carey, M., Moore, D. &Prior, C. — 1993. The enhanced infectivity ofMetarhizium flavoviride in oil formulations to desert locusts at low humidities. —Ann. Appl. Biol., 122, 145–152.Google Scholar
  3. Fargues, J., Maniania, N.K., Delmas, J.C. &Smits, N. — 1992. Influence de la température sur la croissancein vitro d'hyphomycètes entomopathogènes. —Agronomie, 12, 557–564.Google Scholar
  4. Glare, T.R. &Milner, R.J. — 1991. Ecology of entomopathogenic fungi. — Handbook of Applied Mycology, Vol. 2: Humans, Animals, and Insects (D.K. Ajello &K.G. Mukerji, eds.),Marcel Dekker, New York, 547–612.Google Scholar
  5. Jenkins, N.E. & Prior, C. — 1995. Growth and formation of true conidia byMetarhizium flavoviride in a simple liquid medium. —Mycol. Res., 97 (in press).Google Scholar
  6. Johnson, D.L. &Goettel, M.S. — 1993. Reduction of grasshopper populations following field application of the fungusBeauveria bassiana. —Biocontrol Sci. & Technol., 3, 165–175.Google Scholar
  7. Kleespies, R. — 1993. Untersuchungen zur Biologie und Anwendung des entomopathogenen PilzesMetarhizium anisopliae (Metsch.) Sorokin zur biologischen Bekämpfung afrikanischer Wanderheuschrecken. —PhD thesis, TH Darmstadt., 348 pp.Google Scholar
  8. Kleespies, R.G. &Zimmermann, G. — 1992. Production of blastospores by three strains ofMetarhizium anisopliae (Metch.) Sorokin in submerged culture. —Biocontrol Sci. & Technol., 2, 127–135.Google Scholar
  9. Latch, G.C.M. — 1965.Metarhizium anisopliae (Metschnikoff) Sorokin strains in New Zealand and their possible use for controlling pasture-inhabiting insects. —New Zealand J. agric. Res., 8, 384–396.Google Scholar
  10. Müller-Kögler, E. — 1965. Pilzkrankheiten bei Insekten. —P. Parey, Berlin & Hamburg, 444 p.Google Scholar
  11. Prior, C. — 1992. Discovery and characterization of fungal pathogens for locust and grasshopper control. —Biological control of locusts and grasshoppers (Lomer, C.L. &Prior, C. eds.), CAB International, Wallingford, 159–180.Google Scholar
  12. Prior, C. &Greathead, D.J. — 1989. Biological control of locusts: the potential for the exploitation of pathogens. —FAO Plant Protection Bull., 37, 37–48.Google Scholar
  13. Roberts, D. W. & Campbell, A.S. — 1977. Stability of entomopathogenic fungi. —Environmental stability of microbial insecticides (Ignoffo, C. M. & Hostetter, D. L. eds.), Misc. Publ. Entomol. Soc. Am. 10, 19–76.Google Scholar
  14. Scherer, R, Bateman, R.P., Moore, D. & McClatchie, G.V. — 1992. Control of the migratory locustLocusta migratoria capito in Madagascar: the potential for the use of a myco-pesticide. —Brighton Crop Protection Conference — Pests and Diseases, 357–362.Google Scholar
  15. Tulloch, M. — 1976. The genusMetarhizium. —Trans. Br. Mycol. Soc., 66, 407–411.Google Scholar
  16. Zimmermann, G. — 1986. The “Galleria-bait method” for detection of entomopathogenic fungi in soil. —Z. angew. Ent., 102, 213–215.Google Scholar
  17. Zimmermann, G. — 1993. The entomopathogenic fungusMetarhizium anisopliae and its potential as a biocontrol agent. —Pesticide Science, 37, 375–379.Google Scholar
  18. Zimmermann, G., Zelazny, B., Kleespies, R. & Welling, M. — 1994. Biological control of African locusts by entomopathogenic microorganisms. — New trends in locust control (S. Krall & H. Wilps, eds.), Schriftenreihe der GTZ 245, Eschborn, 127–138.Google Scholar

Copyright information

© Lavoisier Abonnements 1994

Authors and Affiliations

  • M. Welling
    • 1
  • G. Nachtigall
    • 1
  • G. Zimmermann
    • 1
  1. 1.Federal Biological Research Centre for Agriculture and ForestryInstitute for Biological ControlDarmstadtGermany

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