Abstract
The virulence of the DAT F-001 isolate ofMetarhizium anisopliae forAdoryphorus couloni was tested by exposing final instar larvae (L3) to concentrations of 101 to 107 spores/g in a sand-peat mix for up to 112 days at 20°±2°C. All concentrations were pathogenic to L3 larvae and survival was dependent on concentration and correlated with exposure time. The time to kill 50% of the treated larvae (LT50) ranged from 18.9 days (107 spores/g) to 82.7 days (101 spores/g). The effect of DAT F-001 on feeding by L3 larvae was determined by comparing the production and weight of frass pellets/larva/day of untreated control larvae with larvae rolled in sporulating cultures ofM. anisopliae DAT F-001 and DAT F-054 (low virulence). Exposure to, and infection byM. anisopliae DAT F-001 had minimal impact on larval feeding. L3 larvae continued to feed at the same rate as both untreated and DAT F-054 treated larvae virtually until they died.
Résumé
La virulence deM. anisopliae DAT F-001 pourA. couloni a été analysée en exposant des larves L3 à des concentrations de 101 à 107 spores/g dans un mélange de sable et de tourbe pendant un maximum de 112 jours à une température de 20°±2°C. Toutes les concentrations sont pathogènes pour les larves L3; la survie dépendait de la concentration et est en corrélation avec le temps d'exposition. Les LT50 sont compris entre 18,9 jours (107 spores/g) et 82,7 jours (101 spores/g). L'effet de DAT F-001 sur l'alimentation des larves L3 a été évalué en comparant la production et le poids des fèces/larve/jour des larves non-traitées avec des larves roulées dans des cultures sporulées deM. anisopliae DAT F-001 et DAT F-054 (faible virulence). L'exposition àM. anisopliae DAT F-001 suivie de l'infection a peu d'effet sur l'alimentation des larves. Les larves L3 continuent à s'alimenter au même rythme que les larves non-traitées de DAT F-054, quasiment jusqu'à leur mort.
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Rath, A.C., Worledge, D. The effect ofMetarhizium anisopliae (DAT F-001) concentration and exposure time on the survival of the subterranean pasture pest,Adoryphorus couloni (Col.: Scarabaeidae) . Entomophaga 40, 35–45 (1995). https://doi.org/10.1007/BF02372679
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DOI: https://doi.org/10.1007/BF02372679