Abstract
In vitro rearing of the aphid endoparasitoidLysiphlebus fabarum (Marshall) (Hymenoptera, Braconidae) was attempted. Successful permanent cultures ofAphis fabae Sc. andMyzus persicae Sulz. cells were not obtained. Therefore, parasitoid larvae were reared in 2 unnatural media rone of which included cells ofCeratitis capitata Wied. (Diptera, Trypetidae). A group of larvae was reared in a substrate to which parasitoid teratocytes had been added. SinceLysiphlebus fabarum females did not oviposit into paraffin droplets including the substrates, the larvae were directly transferred from parasitized aphids into the rearing media. Several larvae reached the final instar, but only 2 out of the 48 tested in the 3 substrates became adults. The meaning of teratocytes inin vitro rearing of Aphidiine, Braconids is discussed.
Résumé
Un essai d'élevagevitro de l'endoparasitoïdeLysiphlebus fabarum (Marshall) a été effectué.
La difficulté d'obtenir des cultures cellulaires permanentes d'Aphis fabae. Sc., a orienté les recherches vers l'élevage des larves dans des substrats inhabituels. On a aussi réalisé des essais en utilisant une lignée cellulairein vitro deCeratitis capitata (Wied.).
Un groupe de larves a été élevé dans un milieu auquel avaient été ajoutés les tératocites du parasitoïde.
Les femelles deLysiphlebus fabarum n'ayant pas pondu dans les capsules de paraffine renfermant le milieu biologique, des larves prélevées dans les aphides parasités y ont été directement transférées.
Plusierus larves ont atteint la maturité au cours de mues successives normales, mais seulement deux d'entre elles sur 48 ont donné naissance à des adultes.
La significantion des tératocites parasitaires dans l'élevagein vitro, des hyménoptères Braconides est discuté.
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This work was supported by a grant from the italian Ministry of Education (M.P.I. 40%).
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Rotundo, G., Cavalloro, R. & Tremblay, E. In vitro rearing ofLysiphlebus fabarum [Hym.: Braconidae] . Entomophaga 33, 261–267 (1988). https://doi.org/10.1007/BF02372615
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DOI: https://doi.org/10.1007/BF02372615