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Production ofbeauveria bassiana [Deuteromycotina. Hyphomycetes] sympoduloconidia in submerged culture

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Abstract

Submerged conidiation of the entomogenous HyphomyceteBeauveria bassiana is reported.

Conidiogenous cells produce sympoduloconidia on conidiogenous cells in liquid shaker culture; hyphal bodies and mycelium fragments are also produced. The morphology of these fungal structures is discussed and illustrated.

Several simple liquid media are tested for the production of conidia and hyphal bodies.

Maximum yields of conidia (170×106 conidia/ml) are produced in a medium consisting of sucrose (2%) — yeast extract (0.5%) and basal salts, and maximum yields of hyphal bodies (740×106 hyphal bodies/ml) in a sucrose (2.5%) — yeast extract (2.5%) medium.

Résumé

La production de conidies de l'hyphomycète entomopathogèneBeauveria bassiana en culture liquide est exposée.

Les cellules conidiogènes produisent des sympoduloconidies sur cellules conidiogènes en fermentation liquide; des corps hyphaux et des fragments de mycelium sont produits également. La morphologie de ces structures fongiques est discutée et illustrée.

Plusieurs milieux liquides simples sont essayés pour produire des conidies et des corps hyphaux.

Les productions maximales de conidies (170×106 conidies/ml) sont obtenues dans un milieu composé de 2% de sucrose et de 0,5% d'extrait de levure et de sels et celles de corps hyphaux (740×106 corps hyphaux/ml) dans un milieu composé de 2,5% de sucrose et de 2,5% d'extrait de levure.

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  1. M. C. Rombach

    Present address: Seconded to Entomology Department, International Rice Research Institute, P.O. Box 933, Manila, the Philippines

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  1. Insect Pathology Resource Center, Boyce Thompson Institute for Plant Research at Cornell, Tower Road, 14853, Ithaca, New York, U.S.A.

    M. C. Rombach

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Rombach, M.C. Production ofbeauveria bassiana [Deuteromycotina. Hyphomycetes] sympoduloconidia in submerged culture. Entomophaga 34, 45–52 (1989). https://doi.org/10.1007/BF02372586

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  • DOI: https://doi.org/10.1007/BF02372586

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