Summary
A glycan was obtained from the periplasmic space ofRhizobium trifolii cells by saccharose-osmotic shock method. This glycan was composed of only β-1,2-linked glucose, having no end groups (reducing and non-reducing) and branching points. It was concluded that this glucan has a structure of unbranched cyclic form.
When the cyclic β-1,2-glucan was added to the seedlings of white clover as a host plant withR. trifolii, it promoted not only the infection thread formation but also the nodule formation. In the culture medium containing the cyclic β-1,2-glucan, cells were found agglutinated during the middle logarithmic phase of growth, and their morphological changes were recognized as cell elongation or swelling in the middle region of a cell when observed in scanning electron microscope (SEM).
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Abe, M., Amemura, A. & Higashi, S. Studies on cyclic β-1,2-glucan obtained from periplasmic space ofRhizobium trifolii cells. Plant Soil 64, 315–324 (1982). https://doi.org/10.1007/BF02372514
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DOI: https://doi.org/10.1007/BF02372514