, Volume 39, Issue 2, pp 159–169 | Cite as

Heterogeneity of twoBeauveria bassiana strains revealed by biochemical tests, protein profiles and bio-assays ofLeptinotarsa decemlineata (Col.: Chrysomelidae) andColeomegilla macultata lengi (Col.: Coccinellidae) larvae

  • S. I. Todorova
  • J. -C. Côté
  • P. Martel
  • D. Coderre


Biochemical profiles on API Rapid CH* strips and protein profiles on polyacrylamide gels in the presence of sodium dodecyl sulfate were used to distinguish two strains of the entomopathogenic fungusBeauveria bassiana (Balsamo) Vuillemin, ARSEF 2991 and ATCC 44860. Next, the toxicity of these two strains was determined at concentrations of 102, 104, 106 and 108 blastospores/ml on larvae of the Colorado potato beetleLeptinotarsa decemlineata Say (Coleoptera: Chrysomelidae) and of its predator, the spotted ladybird beetle,Coleomegilla maculata lengi Timberlake (Coleoptera: Coccinellidae).

Both strains were highly toxic toL. decemlineata larvae. However, the two strains exhibited different levels of toxicity forC. maculata larvae: ARSEF 2991 was toxic, whereas ATCC 44860 caused little coccinellid larval mortality.


Entomopathogenic fungus strain blastospores larval mortality Coleomegilla maculata lengi Leptinotarsa decemlineata 


Les profils biochimiques sur galeries API Rapid CH* et les profils protéiques sur gels de polyacrylamide ont été utilisés pour distinguer deux souches du champignon entomopathogèneBeauveria bassiana (Balsamo) Vuillemin. La toxicité de ces deux souches a été déterminée à des concentrations de 102, 104, 106 et 108 blastospores/ml sur des larves du doryphore,Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae) et de la coccinelle maculéeColeomegilla maculata lengi Timberlake (Coleoptera: Coccinellidae).

Les deux souches deB. bassiana se sont avérées actives à l'égard des larves deL. decemlineata. Toutefois la souche ARSEF 2991 s'est avérée pathogène pour les larves deC. maculata, alors que la souche ATCC 44860 a provoqué une faible mortalité des larves.


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Copyright information

© Lavoisier Abonnements 1994

Authors and Affiliations

  • S. I. Todorova
    • 1
  • J. -C. Côté
    • 2
  • P. Martel
    • 2
  • D. Coderre
    • 1
  1. 1.Département des Sciences BiologiquesUniversité du Québec à MontréalMontréalCanada
  2. 2.Station de RecherchesAgriculture CanadaSt-Jean-sur-RichelieuCanada

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