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Établissement d'une nouvelle lignée cellulaire (IAFEs-1) a partir d'ovarioles d'Euxoa scandens [Lep.: Noctuidae]

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Résumé

Une nouvelle lignée cellulaire stable (IAFEs-1) des ovarioles de chrysalides du lepidoptèreEuxoa scandens Riley a été obtenue. La forme prédominante des cellules est sphérique et le doublement de la population cellulaire s'effectue en 18 et 24 h en utilisant respectivement 20 et 10% de sérum de veau foetal. Cette lignée est sensible à divers virus des polyédroses nucléaires et cytoplasmiques.

Summary

A new continuous cell line designated IAFEs-1 was obtained from ovaries ofEuxoa scandens Riley in order to study the replication and the productionin vitro of viruses infecting this cutworm. Predominant cell morphology is spherical and cell population doubling time is 18 and 24 h using respectively 20 and 10% fetal calf serum. Susceptiblity of the cell line to several nuclear and cytoplasmic polyhedrosis viruses is demonstrated.

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References

  • Arella, M. — 1983. Replication en culture cellulaire du virus de la polyédrose cytoplasmique d'Euxoa scandens, Riley [Noctuidae: Agrotinae]. —Thèse Ph.D., Institut Armand-Frappier, Université du Québec, Laval-des-Rapides, Canada, 227 p.

    Google Scholar 

  • Bellemare, N. &Belloncik, S. — 1981. Etudes au laboratoire des effets d'une polyédrose cytoplasmique sur le ver-gris blancEuxoa scandens [Lepidoptère: Noctuidae, Agrotinae]. —Ann. Soc. Entomol. Québec., 26, 28–40.

    Google Scholar 

  • Belloncik, S. &Bellemare, N. — 1980. Polyèdes du CPV d'Euxoa scandens [Lepidoptera: Noctuidae] produitsin vivo et sur cellules cultivéesin vitro. Etudes comparatives —Entomophaga, 25, 199–207.

    Google Scholar 

  • Brown, S.E. &Knudson, D.L. — 1980. Characterization of invertebrate cell lines. III Isozyme analyses employing cellulose-acetate electrophoresis. —In vitro, 16, 829–832.

    CAS  Google Scholar 

  • Chen, T.R. — 1977.In situ detection of mycoplasma contamination in cell cultures by fluorescent Hoechst 33258 strain. —Exp. Cell. Res., 104, 255–262.

    Article  CAS  PubMed  Google Scholar 

  • Erno, H. &Stipkovits, L. — 1973. Bovine mycoplasma: cultural and biochemical studies. — I.Acta Vet. Scand., 14, 436–449.

    CAS  Google Scholar 

  • Grace, T.D.C. — 1962. Establishment of a mosquito line (Aedes aegypti L.) cells grownin vitro. —Nature, 211, 366–367.

    Google Scholar 

  • Grancher-Barray, S., Boisvert, J. &Belloncik, S. — 1981. Electrophoretic characterization of proteins and RNA of a cytoplasmic polyhedrosis virus (CPV) fromEuxoa scandens. —Arch. Virol., 70, 55–61.

    Article  CAS  PubMed  Google Scholar 

  • Quiot, J.M. — 1976. Etablissement d'une lignée cellulaire (SCLd135) à partir du lépidoptèreLymantria dispar L. —CR Acad. Sci., Paris, 282, 465–468.

    Google Scholar 

  • Quiot, J.M. &Belloncik, S. — 1977. Caractérisation d'une polyédrose cytoplasmique chez le lepidoptèreEuxoa Scandens Riley [Noctuidae: Agrotinae] Etudesin vivo etin vitro. —Arch. Virol., 55, 145–153.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Centre de recherche en virologie, Institut Armand-Frappier, Université du Québec, 531, Boulevard des Prairies, H7N 4Z3, Laval-des-Rapides, Québec, Canada

    S. Belloncik

  2. Station de recherches de pathologie comparée, St Christol Les Alès, France

    J. M. Quiot

Authors
  1. S. Belloncik
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  2. J. M. Quiot
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  3. M. Arella
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  4. J. Lecomte
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Belloncik, S., Quiot, J.M., Arella, M. et al. Établissement d'une nouvelle lignée cellulaire (IAFEs-1) a partir d'ovarioles d'Euxoa scandens [Lep.: Noctuidae] . Entomophaga 30, 51–54 (1985). https://doi.org/10.1007/BF02372282

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  • DOI: https://doi.org/10.1007/BF02372282

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