Entomophaga

, Volume 28, Issue 2, pp 179–184 | Cite as

Ovicidal activity ofMetarhizium anisopliae isolate CM-14 on the coffee leaf miner.Perileucoptera coffeella [Lep: Lyonetiidae]

  • Amador Villacorta
Article

Abstract

Bioassays were conducted to determine the susceptibility of eggs ofPerileucoptera coffeella Guerin-Meneville to different spore doses ofMetarhizium anisopliae (Metsch.) isolate CM-14 at 28±1°C and 95±5% R. H. The fungi was highly virulent against eggs. Larval mortality by the fungi was 100% in all cases. The larvae were infested inside the gallery or when they were seeking site for pupation. LD50 of fungi to eggs was determined to be of 4.6 mg/100 ml water.

Keywords

Plant Pathology Mycose Larval Mortality Leaf Miner Ovicidal Activity 

Résumé

Des essais biologiques ont été conduits pour déterminer la sensibilité des œufs dePerileucoptera coffeella Guérin-Meneville à différentes doses deMetarhizium anisopliae (Metsch.) Sorokin souche CM-14, à 28±1°C et 85±5% H.R.

Le champignon s'est montré hautement virulent pour les œufs. On a obtenu une mortalité de 100% des chenilles à toutes les concentrations étudiées. Les chenilles mortes de mycose ont été trouvées à l'intérieur des galeries ou sur les feuilles quand elles étaient à la recherche d'abris pour se nymphoser.

La DL-50 du champignon pour les œufs deP. coffeella est de 4,6 mg/100 ml d'eau.

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Copyright information

© Lavoisier Abonnements 1983

Authors and Affiliations

  • Amador Villacorta
    • 1
  1. 1.Instituto Agronômico do Paraná (IAPAR)LondrinaBrazil

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