Entomophaga

, Volume 25, Issue 3, pp 301–311 | Cite as

Zur aufbewahrung entomogener pilzkulturen

  • E. Müller-Kögler
  • G. Zimmermann
Article

Zusammenfassung

Die Lebensdauer verschiedener insektenpathogener Entomophthoraceen undFungi imperfecti, des imperfekten Stadiums vonCordyceps militaris(Fr.) Link und von 2 Trichomyceten wurde mit folgenden einfachen Methoden getestet: In steriler Erde bei 4°C, in steriler 0,675% iger NaCl-Lösung bei 4°C, als Kulturen bei 4°C und −18°C sowie unter Paraffinöl bei 4°C. Die folgenden Methoden waren für die untersuchten Arten am günstigsten:
  1. 1.

    Die empfindlichenEntomophthora-Arten,E. muscae(Cohn) Fres,E. aulicae(Reich.) Sorok,E. exitialisHall & Dunn undE. obscuraHall & Dunn, liess en sich mit verschiedenen Methoden nur für einige Wochen oder Monate am Leben halten. Die letzten 3 Arten überlebten 6 Monate, und zwar auf koaguliertem Eidotter bei 4°C.

     
  2. 2.

    Conidiobolus (Entomophthora) coronatus(Cost.) Tyrrell & MacLeod war in steriler Erde bei 4°C noch nach 4 Jahren lebensfähig.

     
  3. 3.

    VonE. virulentaHall & Dunn liess sich der Stamm Nr. 1 mit verschiedenen Methoden 4–5 Jahre lang aufbewahren. Der Stamm Nr. 2 zeigte schlechtere Lagerungseigenschaften, war aber in steriler Erde bei 4°C nach 8 Jahren noch am Leben.

     
  4. 4.

    Smittium inopinatum(Manier, Rioux & Whisler) Manier überlebte in steriler 0,675% iger NaCl-Lösung 2 Jahre.

     
  5. 5.

    Verglichen mit den Entomophthoraceen und Trichomyceten ist die Lagerung der insektenpathogenenFungi imperfecti (Beauveria bassiana(Bals.) Vuill.,B. tenella(Delacr.) Siem.,Hirsutella sp.,Metarhizium anisopliae(Metsch.) Sorok.,Nomuraea rileyi(Farlow) Samson,Paecilomyces farinosus (Dicks. exFr.)Brown & Smith,Sorosporella uvella(Krass.) Giard) und des imperfekten Stadiums vonCordyceps militaris problemloser. Am geeignetsten erwies sich das Einfrieren ihrer Kulturen bei −18°C. Sie überlebten hier 5 Jahre, die vonB. bassiana, Hirsutella sp. undP. farinosus sogar 9 Jahre.Hirsutella sp. liess sich auch in 0,675% iger NaCl-Lösung 9 Jahre lebend aufbewahren.

     

Verglichen mit den Ausgangskulturen waren Wachstum und Sporulation fast aller Testabimpfungen unverändert. Auf Grund dieser Ergebnisse halten wir die meisten insektenpathogenenFungi imperfecti bei −18°C und zweijährigen Abimpfungen. Nebenher läuft eine Serie bei 4°C im Kühlschrank und jährlicher Abimpfung. Die Entomophthoraceen undSmittium spp. werden dagegen alle 4 Wochen abgeimpft und bei +18°C aufbewahrt.

Summary

The longevity of several entomopathogenicEntomophthoraceae andFungi imperfecti as well as two species of Trichomycetes and the imperfect state ofCordyceps militaris(Fr.) Link, was tested by the following simple preservation methods: storage in sterile soil at 4°C, in sterile aqueous solution of 0,675% NaCl at 4°C, as cultures in a refrigerator at 4°C with and without a layer of mineral oil and at −18°C. The most suitable techniques for preservation of the tested species were as follows:
  1. 1.

    The sensitiveEntomophthora species,E. muscae(Cohn) Frfs.,E. aulicae(Reich.) Sorok,E. exitialisHall & Dunn andE. obscuraHall & Dunn, could only be stored for some weeks or months with the various methods tested. The latter 3 species survived 6 months on coagulated egg yolk at 4°C.

     
  2. 2.

    Conidiobolus (Entomophthora) coronatus(Cost.) Tyrrell & MacLeod survived 4 years in sterile soil at 4°C.

     
  3. 3.

    Strain no. 1 ofE. virulentaHall & Dunn could be preserved 4–5 years with different methods. In general, strain no. 2 was more sensitive but was still alive in sterile soil at 4°C after 8 years.

     
  4. 4.

    Smittium inopinatum(Manier, Rioux & Whisler) Manier survived 2 years in a sterile solution of 0,675% NaCl.

     
  5. 5.

    The preservation of the entomopathogenicFungi imperfecti (Beauveria bassiana(Bals.) Vuill.,B. tenella(Delacr.) Siem.,Hirsutella sp.,Metarhizium anisopliae(Metsch.) Sorok.,Nomuraea rileyi(Farlow) Samson,Paecilomyces farinosus (Dicks exFr.)Brown & Smith,Sorosporella uvella)(Krass.) Giard, and of the conidial state ofCordyceps militaris is quite easier than that ofEntomophthoraceae and Trichomycetes. Storage at −18°C proved to be the best method. All strains survived 5 years, cultures ofB. bassiana, Hirsutella sp. andP. farinosus were still alive after 9 years. — Storage ofHirsutella sp. was also possible in sterile solution of 0,675% NaCl even for 9 years.

     

After the preservation periods sporulation and growth of the subcultures were comparable to that of the original cultures. Most strains of entomopathogenicFungi imperfecti are stored, therefore, in our laboratory at −18°C, subculturing every 2 years. Furthermore, one series is kept in a refrigerator at 4°C, subculturing once a year. For convenience theEntomophthoraceae andSmittium spp., however, are stored at +18°C with transfers to a new substrate at 4 weeks intervals.

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Copyright information

© Le François 1980

Authors and Affiliations

  • E. Müller-Kögler
    • 1
  • G. Zimmermann
    • 1
  1. 1.Institut für biologische SchädlingsbekämpfungBiologische Bundesanstalt für Land- und ForstwirtschaftDarmstadtBundesrepublik Deutschland

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