Advertisement

Entomophaga

, Volume 15, Issue 2, pp 209–222 | Cite as

Effets physiologiques de la toxine thermostable deBacillus thuringiensisBerliner sur les larves et les adultes deDrosophila melanogasterMeigen

  • Jeannine van Herrewege
Mémoires Originaux

Résumé

Les effets de la toxine thermostable deB. thuringiensis, administrée par voic orale, ont été étudiés sur les larves et les adultes deDrosophila melanogaster. Tous les caractères sont sensibles à la toxine et montrent une réponse proportionnelle à sa concentration. Les résultats obtenus permettent de discuter le mode d'action de la toxine et de définir quelques méthodes de dosage biologique.

Summary

Incorporated to an axenic rearing medium, a heat-stable formulation fromBacillus thuringiensis causes numerous physiological modifications inDrosophila.

Larval growth is slowed down and an increase of the whole duration of development is observed. Developmental mortality is high and the LC 50 is about 1 ppm. Female larvae appear more sensitive and the proportion of males in cultures increases with the toxin concentration. Adults, issued from intoxicated larvae, are a little smaller but do not show any teratological abnormalities.

Given to adults, the toxin reduces longevity both in males and females. Fecundity is reduced and fertility decreases after two weeks.

The observed effects are related to the toxin concentration and also to the duration of its action. In many cases an approximate linear relationschip is observed as a function of the logarithm of the concentration. The most useful characters for measuring the biological activity of a toxic preparation are: duration of development, larval and pupal mortality and longevity.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Bibliographie

  1. Barjac, H. de &Dedonder, R. — 1965. Isolement d'un nucléotide identifiable à la toxine thermostable deBacillus thuringiensis variétéBerliner. —C. R. Acad. Sci. Paris,260, 7050–7052.Google Scholar
  2. — — 1968. Purification de la toxine thermostable deB. thuringiensis var.thuringiensis et analyses complémentaires. —Bull. Soc. Chimie biol.,4, 941.Google Scholar
  3. Benz, G. &Perron, J. M. — 1967. The toxic action ofBacillus thuringiensis “exotoxin” onDrosophila reared in yeast containing and yeast free media. —Experientia,23, 871–887.CrossRefPubMedGoogle Scholar
  4. Bliss, C. I. — 1935a. The calculation of the dosage mortality curve. —Ann. applied Biol.,22, 134–167.Google Scholar
  5. Burgerjon, A. &Barjac, H. de — 1960a. Nouvelles données sur le rôle de la toxine soluble thermostable produite parBacillus thuringiensis Berliner. —C. R. Acad. Sci. Paris.,251, 911–912.Google Scholar
  6. Burgerjon, A. & Barjac H. de — 1960 b. Essais préliminaires sur le rôle insecticide de la toxine thermostable produite parBacillus thuringiensis BerlinerCongr. intern. entomol. Vienne, 835–839.Google Scholar
  7. Burgerjon, A. &Biache, G. — 1964. The activity of the heat stable toxin ofBacillus thuringiensis Berliner used in nature against the larvae ofDiprion pini L. —J. Insect Path.,6, 538–541.Google Scholar
  8. — — 1967. Divers effets spéciaux et symptômes tératologiques de la toxine thermostable deBacillus thuringiensis en fonction de l'âge physiologique des insectes. —Ann. Soc. entomol. France,3, 929–952.Google Scholar
  9. Burgerjon, A., Biache, G. &Cals, Ph. — 1969. Teratology of the Colorado potato beetleLeptinotarsa decemlineata, as provoked by larval administration of the thermostable toxin ofBacillus thuringiensis. —J. Invertebrate Path.,14, 274–278.Google Scholar
  10. Burgerjon, A. &Galichet, P. F. — 1965. The effectiveness of the heat stable toxine ofBacillus thuringiensis Berliner on larvae ofMusca domestica Linnaeus. —J. Invertebrate Path.,7, 263–264.Google Scholar
  11. Burgerjon, A., Grison, P. &Kachkouli, A. — 1964 Activity of the heat stable toxin ofBacillus thuringiensis Berliner inLocusta migratoria (Linnaeus) [Locustidae, Orthoptera]. —J. Insect Path.,6, 381–383.Google Scholar
  12. Cantwell, G. E., Knox, D. A. &Michael, A. S. — 1964. Mortality of honey bees,Apis mellifera Linnaeus fed of exotoxin, ofBacillus thuringiensis var.Thuringiensis Berliner. —J. Insect Path.,6, 532–536.Google Scholar
  13. Colloque de pathologie et de lutte microbiologique — 1967. Wageningen (5–10 sept. 1966). —Entomophaga,12, 91–95.Google Scholar
  14. David, J. — 1959. Étude quantitative du développement de la Drosophile élevée en milieu axénique. —Bull. biol.,93, 472–505.Google Scholar
  15. David, J. &Vago, C. — 1966. Influence des toxines de,Bacillus thuringiensis sur divers caractères physiologiques de Drosophiles adultes. —Entomophaga,12, 153–159.Google Scholar
  16. Krieg, A. — 1965b. Über den Biotest vonBacillus thuringiensis Exotoxin mitDrosophila melanogaster. —Ent. Exp. et Appl.,9, 185–190.Google Scholar
  17. Perron, J. M. &Benz, G. — 1968. Effects of yeast fractions on the action of the socalled heat stable exotoxin ofBacillus thuringiensis inDrosophila melanogaster. —J. Invertebrate Path.,10, 379–386.Google Scholar
  18. Smirnoff, W. A. &Berlinguet, L. — 1966. A substance in some commercial preparations ofBacillus thuringiensis var.thuringiensis toxic to sawfly larvae. —J. Invertebrate Path.,8, 376–381.Google Scholar
  19. Van Herrewege, J. — 1969. La toxine deBacillus thuringiensis var.thuringiensis Berliner (sérotype 1): effets surDrosophila melanogaster Meigen. —Thèse 3 e cycle Lyon, 90 pp.Google Scholar
  20. Van Herrewege, J. & David, J. — 1970. Réduction de la longévité de la Drosophile par l'action d'une toxine bactérienne. —Exp. Geront. (sous presse).Google Scholar

Copyright information

© Le François 1970

Authors and Affiliations

  • Jeannine van Herrewege
    • 1
  1. 1.Laboratoire d'Entomologie expérimentale et de GénétiqueEquipe de recherche associée au C.N.R.S.Lyon 7France

Personalised recommendations